Antioxidant Activities of Bingöl Royal Jelly on SH-SY5Y Cells

Abstract

Royal Jelly is a bee product with high protein content is a unique nutrient for the queen honeybee. It leads to a substantial elongation of the lifetime of the queen in comparison to the worker honeybees via anti-inflammatory, anti-oxidant, anti-cancer and anti-microbial properties. Flavonoids naturally have reported to have anticancer activities thanks to their potent antioxidant activity. The antioxidant activity of RJ is attributed to its rich flavonoid content however anti-oxidant activities of the Bingöl RJ has yet to be explored in detail. The goal of the current study was to investigate the antioxidant activities of Bingöl RJ on SH-SY5Y neuroblastoma cells. The results of our study revealed that Bingöl RJ at a concentration of 0.3 mg/ml significantly augmented ROS level in SH-SY5Y cells while 0.5 mg/ml of RJ had almost no effect on ROS levels. The levels of malondialdehyde in SH-SY5Y cells considerably increased in the presence of 0.3 mg/ml RJ while 0.5 mg/ml RJ had no significant impact on MDA levels in SH-SY5Y cells. The results showed that RJ treatment 0.3 mg/ml) significantly lowered the activities of SOD and CAT activity while 0.5 mg/ml of RJ had negligible effect indicating that RJ could protect the cell membranes from radical mediated cell injuries.

Exploring the in vitro potential of royal jelly against glioblastoma and neuroblastoma: impact on cell proliferation, apoptosis, cell cycle, and the biomolecular content

“…In particular, we observed an elevated ROS level in the high dose RJ treated cells, which confirms these findings. Furthermore, elevated ROS and LPO levels were demonstrated in PC3 and MCF-7 cells induced by RJ 49 confirming ROS-induced macromolecular changes stimulated by RJ application.…”

“…Borawska and his colleagues reported that the PBS extract of RJ (ERJ) has a cytotoxic effect on the U87 mg cell line at a dose of 30 μg ml −1 after 72 h. 22 Moreover, 1250 μg ml −1 of the lipophilic extract of RJ significantly decreased the cellular viability of SH-SY5Y cells for 48 h. 23 0.3 mg mL −1 dose of Bingöl RJ has also demonstrated the antioxidant effect on the same cell line via an increased malondialdehyde level and decreased SOD and CAT activity. 49 There is no information about the effect of RJ treatment on the cellular macromolecules in terms of lipid, protein and nucleic acid concentrations, lipid and protein structural changes, protein carbonylation and phosphorylation levels, etc . Hence, the current study was conducted to elucidate that RJ triggered these molecular changes.…”

Evaluation of the Biological Activities of Royal Jelly on Prostate and Breast Cancer Cells

“…While 0.5 mg/ml RJ had no discernible effect on MDA levels in SH-SY5Y cells, the addition of 0.3 mg/ml RJ significantly enhanced the levels of malondialdehyde in those cells. The findings demonstrated that RJ treatment at 0.3 mg/ml dramatically decreased SOD and CAT activity while having little to no effect at 0.5 mg/ml, suggesting that RJ could protect cell membranes from radical-mediated cell damage [34,35].…”