Antioxidant Activities of Cedrela sinensis Hydrolysates Prepared Using Various Enzymes

오민희,; 장혜림,; 임예진,; 윤경영,

doi:10.9721/kjfst.2015.47.4.413

Cited by 5 publications

(2 citation statements)

References 22 publications

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“…The signifi cantly higher yield of P-EAS than that of P-EAV (P<0.05) suggested that Shearzyme degraded the cell wall of C. sinensis more effi ciently than Viscozyme. Shearzyme, from Aspergillus oryzae, mainly shows endo-1,4--β-xylanase activity and may also have cellulase, xylanase, and β-glucanase activities, while Viscozyme is a multifunctional enzyme that contains hemicellulase, β-gluconase, arabinase, and xylanase [Oh et al, 2015].…”

Section: Yield Of Crude Polysaccharide Fractions and Their Monosacchamentioning

confidence: 99%

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Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods

Oh¹,

Yoon²

2018

Pol. J. Food Nutr. Sci.

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in the prevention of oxidative damage in human cells [Wang et al., 2012a]. In general, polysaccharides are bound to other components like protein, lipids, lignin, and some inorganic minerals in cells. Thus, polysaccharide extraction and isolation from various sources is the most important task in the investigation and application of bioactive polysaccharides. The extraction technique used may signifi cantly infl uence the yield, physical characteristics, and biological activities of the polysaccharides. Hot-water extraction (HWE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction, enzyme-assisted extraction (EAE), maceration, and heat refl ux with solvents are the main methods for polysaccharide extraction from natural resources [Fan et al., 2015]. HWE is traditionally used because it is a convenient, fast, environment-friendly, and economical technology [Liu et al., 2016]. UAE has been widely used to extract bioactive components from various plant materials based on mass transfer intensifi cation, cell collapse, increased penetration, and capillary effects [Yin et al., 2016]. EAE can be used to release natural functional compounds from plant materials, owing to cell wall degradation that facilitates polysaccharide dissolution into the solvent [Pan et al., 2015]. In this study, crude polysaccharides were extracted from C. sinensis by various extraction methods and their health-related properties were measured in order to improve their availability to functional food materials. The crude polysaccharide fractions from C. sinensis were extracted using hot water, ultrasonic-assisted, and enzyme-assisted methods. Biological activities such as antioxidant activity, α-amylase in

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Section: Yield Of Crude Polysaccharide Fractions and Their Monosacchamentioning

confidence: 99%

“…Cedrela sinensis, which belongs to the Meliaceae family, is a tall tree that grows in Korea and China and has been used to treat enteritis, dysentery, and itch in oriental medicine [Oh et al, 2015]. Young C. sinensis leaves and sprouts have also been used as functional foods in Korean cuisine for decades.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods

Oh¹,

Yoon²

2018

Pol. J. Food Nutr. Sci.

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Optimization of enzyme treatment conditions for extraction of dietary fiber from Opuntia humifusa stem

Jang¹,

Kim²,

Yoon³

et al. 2020

Korean J. Food Preserv.

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This study aimed to optimize the hydrolysis conditions required for extraction of dietary fiber from Opuntia humifusa stem using Viscozyme ® (MilliporeSigma, St. Louis, MO, USA) with response surface methodology (RSM). A central composite design was applied to examine the effects of the independent variables, enzyme concentration (0.5-2.5%, X 1) and reaction time (1-5h, X 2). The hydrolysis conditions were optimized using total yield, total dietary fiber (TDF), water-soluble dietary fiber (SDF), and water-insoluble dietary fiber (IDF) contents of hydrolysates as dependent variables. The total yield was 39.9-53.2% and the coefficient (R 2) of the derived equation from the response surface regression for total yield was 0.9073. The TDF and SDF contents in all hydrolysates were 29.02-43.82% and 27.25-40.68%, respectively, which were within the 1% level. The IDF contents in all hydrolysates were 0.98-3.80% (R 2 0.8886) and with in 5% significance. The optimal hydrolysis conditions comprised 1.0-1.8% Viscozyme ® and an incubation period of 1.0-1.8 h. Our results suggested that O. humifusa could be a source that might extend the application of dietary fiber in functional food industries.

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Antioxidant activity of water-soluble polysaccharide extracts produced from perilla seed cake by enzymatic hydrolysis

Hwang¹,

Kim²,

Yoon³

2021

Korean J. Food Preserv.

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This study was conducted for the production of water-soluble polysaccharide (WSP) extracts from perilla seed cake (PSC), a byproduct of food processing, by using commercial enzymes. PSC was divided into cellulose fraction (CF) and hemicellulose fraction (HF), and the enzymatic hydrolysis conditions of each fraction were determined for the production of WSP extracts. Afterwards, the antioxidant activity of these WSP extracts was measured. The hydrolysis conditions of the CF for producing WSP extracts were pH 5.0, 50℃, and 30 units of Celluclast while those of HF were pH 4.0, 50℃, and 30 units of Viscozyme. The yield of WSP extracts increased from 176 g/kg at 0 h of hydrolysis to 233.1 g/kg after 72 h of hydrolysis. Futhermore, the polyphenol content of WSP extracts from CF and HF were significantly higher after enzymatic hydrolysis for 24 h, but decreased after 48 h of hydrolysis. In addtion, WSP extracts obtained after 24 h of hydolysis showed the lowest IC 50 values for hydroxy radical scavenging activity, reducing power activity, and superoxide dismutase-like activity. From the result, WSP extracts with antioxidant activity could be effectively extracted by enzymatic hydrolysis, and the extracts are considered to be used as a functional substance in the food industry. Key words：perilla seed cake, agricultural by-product, water-soluble polysaccharides, antioxidant activity, enzymatic hydrolysis

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Antioxidant Activities of Cedrela sinensis Hydrolysates Prepared Using Various Enzymes

Cited by 5 publications

References 22 publications

Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods

Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods

Optimization of enzyme treatment conditions for extraction of dietary fiber from Opuntia humifusa stem

Antioxidant activity of water-soluble polysaccharide extracts produced from perilla seed cake by enzymatic hydrolysis

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