Antioxidant Activity of 96% Ethanol Extract of Pepaya Jepang Leaves (Cnidoscolus aconitifolius (Mill.) I. M. Johnst) Using DPPH Method (1,1-diphenyl-2-picrylhydrazyl)

Abstract

Free radicals in the human body can be helped neutralized by natural antioxidants derived from plants. Pepaya Jepang (Cnidoscolus aconitifolius (Mill.) I.M.Johnst) contains antioxidant compounds such as flavonoids and polyphenols. This study examined the antioxidant activity of 96% ethanol extract of C. aconitifolius leaves. Maceration of C. aconitifolius leaves using 96% ethanol solvent, phytochemical screening, and antioxidant activity assay was carried out with 1,1-Diphenyl-2-Picryl Hydrazil (DPPH) on 96% ethanol extract of C. aconitifolius. Extraction obtained a yield of 16.1834%, and phytochemical screening on the 96% ethanol extract of C. aconitifolius contained flavonoids, tannins, saponins, phenols, alkaloids, and steroids. The antioxidant activity assay on 96% ethanol extract of C. aconitifolius had an IC50 value of 145.3855 ppm and an IC50 value of vitamin C of 7.0806 ppm. The Antioxidant Activity Index (AAI) of 96% ethanol extract of C. aconitifolius was 0.4127, and the AAI of vitamin C was 8.4739. Based on the results obtained, although in a weak classification, a 96% ethanol extract of C. aconitifolius leaves has antioxidant activity.

Identification of Bioactive Compounds and Antibacterial Activity of Limeberry (Triphasia trifolia) Fruit Ethanol Extract against Staphylococcus aureus

“…A previous study reported that 96% ethanol was used to extract various bioactive compounds, including alkaloids and polyphenol compounds, such as flavonoids and tannin. 22 A previous study also reported that different ethanol concentrations have been widely used in bioactive compound extraction. 23 Ethanol, or mixture of ethanol and water, is widely used as a solvent due to its safety and it is also categorized as a food-grade solvent.…”

“…21 Additionally, a 96% ethanol solvent was also used to extract bioactive compounds from Cnidoscolus aconitifolius leaf. 22 According to the qualitative phytochemical analysis, this extract contains alkaloids, flavonoids, and tannins (Table 1). A previous study reported that 96% ethanol was used to extract various bioactive compounds, including alkaloids and polyphenol compounds, such as flavonoids and tannin.…”

Antioxidant, Total Phenolic, and Total Flavonoid of 70% Ethanol Extract of Avocado Seeds (Persea americana Mill.)

“…Antioxidant activity determined by DPPH assay of P. americana seeds maceration and reflux method refers to the procedure 23 with some modifications. 24 Preparation of a standard solution by weighing 5 mg of vitamin C dissolved in 50 mL of 96% ethanol to obtain a concentration of 100 ppm. Formulation of standard vitamin C curves with series concentrations of 2, 4, 6, 8, 10 µg/mL.…”