Antioxidant activity of devil’s claw cell biomass and its active constituents

Anti-inflammatory activity of Devil’s claw in vitro systems and their active constituents

“…Prior to the experiments reported here the callus culture was supported on Linsmayer and Skoog medium (LS; Linsmayer & Skoog, 1965) with 0.2 mg/l 2,4-dichlorophenoxyacetic acid, while the hairy root culture was supported on Murashige and Skoog medium (MS; Murashige & Skoog, 1962), in both cases supplemented with 30 g/l sucrose and solidified by plant agar (5.5 g/l, Duchefa, The Netherlands). The maintenance conditions for these cultures, and the preparation and inoculation of the cell suspension and hairy root cultures are described elsewhere (Georgiev, Heinrich, Kerns, Pavlov, & Bley, 2006;Georgiev et al, 2010). In the experiments the cell suspension culture and hairy roots were cultured submerged on a shaker (110 rpm), at 26°C, in the dark.…”

“…To evaluate the anti-inflammatory effects we applied a scheme for isolating the bioactive metabolites, involving low pressure chromatographic separation, as described by Georgiev et al (2010). Portions (50 g) of the freeze-dried suspension cell biomass and hairy roots were individually homogenised with sea sand using a mortar and pestle, then extracted by incubation in pure MeOH for 48 h. The resulting extracts from the cell biomass and hairy roots, designated fractions CME-CB and CME-HR, respectively, were filtered and the solvent and volatile components were evaporated in vacuo, leaving dry residues of 23.4 and 20.13 g, respectively.…”

“…For instance, verbascoside (also known as acteoside; 2) is a phenylethanoid glycoside that has been shown to have strong anti-leukaemic and cytotoxic activity against a murine cell line and anti-inflammatory activity (Diaz et al, 2004;Pettit et al, 1990). Other phenylethanoid glycosides include b-OH-verbascoside (1) and leucosceptoside A (3), which show strong antioxidant activities in several in vitro assays and martynoside (4), which has antimicrobial activity (Dembitsky, 2005;Georgiev et al, 2010).…”

Phytochemical and flow cytometric analyses of Devil’s claw cell cultures

“…1;Georgiev et al 2010;Gyurkovska et al 2010) and hairy root cultures Homova et al 2010). The present study has been undertaken to evaluate the biosynthetic potential of H. procumbens cell suspensions, batchcultivated in shake-flasks, and to compare them as potential sources for commercial production of bioactive secondary metabolites to hairy root cultures of the same species.…”

Antioxidants from Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong

“…Previous chemical and pharmacological studies have preliminarily confirmed that phenylpropanoid glycosides, iridoid glycosides and flavonoids are the main chemical constituents and active substances of this plant [4,5]. These kinds of compounds play an important role in human health owing to their antioxidant activities [6][7][8]. Therefore, the development of convenient and efficient methods to screen and separate antioxidant active substances with higher activity and safety from this plant is highly desirable.…”