Antioxidant Activity of Entrapped alfa-tocopherol and Lutein in PLGA Nanoparticles in Wistar Rats

Miricescu, Daniela; Totan, Alexandra; Stanescu, Iulia Ioana; Didilescu, Andreea Cristiana; Sfeatcu, Ruxandra; Stănescu, Ana Maria Alexandra; Ştefani, Constantin; Greabu, Maria

doi:10.37358/mp.19.3.5221

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…Nps of (PLGA) containing lutein (in the following called Lut Nps) were prepared by the emulsion-solvent evaporation method previously described by Miricescu et al [ 29 , 30 ]. A stock solution 5.5 mg/mL of Lutein (Acros Organics 456160050, Thermo Fisher Scientific, Waltham, MA, USA) (LUT) was prepared in ethanol (96% p.a., CHEMICAL, 603-002-00-5) and sterilized by filtration (0.22 μm Millipore, GSWP04700).…”

Section: Methodsmentioning

confidence: 99%

Lutein Treatment Effects on the Redox Status and Metalloproteinase-9 (MMP-9) in Oral Cancer Squamous Cells—Are There Therapeutical Hopes?

et al. 2021

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Carotenoids loaded in nanoparticles should be regarded as a promising way to increase the availability in healthy cells and to induce apoptosis in cancer. Lutein is a carotenoid that, in contrast to beta-carotene, has no known toxicities. Oral cancer represents one of the most frequent types of cancer world-wide with an incidence rate of about 9% of all types of cancer. Almost 95% of all oral cancers are represented by squamous cell carcinomas (OSCC). The aim of this study was to review and analyse the effects of lutein and Poly(d,l-lactide-co-glycolide) (PLGA) Nps containing lutein (Lut Nps) on oxidative stress biomarkers (OXSR-1, FOXO-3, TAC) and collagen degradation biomarker–MMP-9, in human cells BICR10 of buccal mucosa squamous carcinoma. Lut Nps were prepared by the emulsion-solvent evaporation method. MMP, OXSR-1, TAC, FOXO-3 and MMP-9 were measured in tumour cell lysates by the ELISA technique. Our results have shown that in Lut 100 cells and Lut Nps the OXSR1 (p < 0.001, p < 0.001) and TAC (p < 0.001, p < 0.001) values were significantly higher than in control cells. The Lut 100 and Lut Nps FOXO-3 levels revealed no significant differences versus the control. MMP-9 levels were significantly reduced (p < 0.001) in the Lut Nps cells versus control cells. In our study conditions, lutein and lutein Nps did not trigger an oxidative stress by ROS induction. However, lutein Nps treatment seemed to have a positive effect, by downregulating the MMP-9 levels. Loaded in Nps, lutein could be regarded as a protective factor against local invasiveness, in whose molecular landscape MMPs, and especially MMP-9 are the main actors.

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Section: Methodsmentioning

confidence: 99%

Lutein Treatment Effects on the Redox Status and Metalloproteinase-9 (MMP-9) in Oral Cancer Squamous Cells—Are There Therapeutical Hopes?

et al. 2021

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“…NAFLD molecular pathogenesis is not completely elucidated yet. Obesity should be considered an important risk factor for NAFLD [16][17][18][19]. The liver, the headquarter of important biochemical processes (gluconeogenesis, ureogenesis, oxidative phosphorylation, glycolysis, lipogenesis) is also an important player in the detoxification processes.…”

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confidence: 99%

Hypercaloric Diet and Vitamin E Intake

Radulescu¹,

Miricescu²,

Totan³

et al. 2019

Mat.Plast.

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PLGA (poly-lactic-co-glycolic acid) nanoparticles represent an important synthetic biocomponent that has the potential to be a promising carrier of drugs, proteins, nucleic acids, due to its biodegradability and minimal side effects. The aim of our study was to observe the antioxidant effect of vitamin E loaded in PLGA nanoparticles administered over a period of 3 weeks in Wistar rats treated with a hypercaloric diet. Glutathione (GSH) and malondiadehyde (MDA) biomarkers determined from liver lysate were analyzed to evaluate the oxidative stress (OS) induced by the hypercaloric diet. The results of our study revealed a statistically significant increase for GSH and vitamin E in group 2 of Wistar rats receiving hypercaloric diet and a daily dose of vitamin E versus group 1 (p[0.005). The antioxidant effect of vitamin E was also observed by the statistically significant decrease of MDA in group 2 of Wistar rats compared with group 1. The daily dose of vitamin E has improved the liver redox status of group 2 Wistar rats.

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Glycosylated zein as a novel nanodelivery vehicle for lutein

Chang

Jiao

et al. 2022

Food Chemistry

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Antioxidant Activity of Entrapped alfa-tocopherol and Lutein in PLGA Nanoparticles in Wistar Rats

Cited by 6 publications

References 30 publications

Lutein Treatment Effects on the Redox Status and Metalloproteinase-9 (MMP-9) in Oral Cancer Squamous Cells—Are There Therapeutical Hopes?

Lutein Treatment Effects on the Redox Status and Metalloproteinase-9 (MMP-9) in Oral Cancer Squamous Cells—Are There Therapeutical Hopes?

Hypercaloric Diet and Vitamin E Intake

Glycosylated zein as a novel nanodelivery vehicle for lutein

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