Antioxidant Activity of Goat Milk Yogurt Casein on Dioxin Intoxicated Rat Liver Function

Abstract

Dioxin is toxic contaminant which persistent in the environment. This compound bio-accumulate through food chain and generate adverse effect in human health. Oxidative stress due to dioxin exposure can interfere metabolism process and leads to the development of hepatotoxicity.The aim of this study was to asses the role of casein derived from goat milk yogurt in dioxin-intoxicated rat based on liver function parameters. Substances used were: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) diluted in corn oil and casein from goat milk yogurt. The study included 24 Wistar rats divided into 6 groups: (1) control group, (2) placebo group which given casein 600 mg/kg BW, (3) TCDD group given with TCDD 100 ng/kg BW, (4) group P1 given casein 300 mg/kg BW and TCDD, (5) group P2 given casein 600 mg/kg BW and TCDD, (6) group P3 given casein 900 mg/kg BW and TCDD. The respective dose were given orally for 21 days. Blood serum was collected for transaminases (ALT, AST) and γ-glutamyl transferase (GGT) analysis. Based on serum transaminases activity, there were no significant change on AST and ALT level. Casein yogurt supplementation has significant effect on serum GGT level (p<0,05) and optimum dose was 600 mg/kg BW/day

Effect of hepatogomax on serum alkaline phosphatase, bilirubin, gamma-glutamyl transferase levels in Sprague Dawley rats cirrhosis

“…High GGT levels are caused by inflammation and oxidative stress (Setianingrum & Padaga, 2019). High bilirubin, due to disruption of portal blood flow, leads to inhibition of bilirubin and portosystemic shunting excretion, thus increasing hemolysis (Yusra et al, 2020).…”

“…High GGT levels are associated with inflammation and oxidative stress. Serum GGT catalyzes the first step in the degradation of the extracellular antioxidant glutathione, allowing the precursor amino acids to be reused for intracellular synthesis (Setianingrum & Padaga, 2019). Liver function biomarkers such as ALP, bilirubin, and GGT are used to diagnose liver dysfunction.…”