Antioxidant Activity of Seminal Plasma in Fertile and Infertile Men

Koca, Yüksel; Ozdal, Ozdem Levent; Çelik, Muhammet Cihat; ÜNAL>, Şenay; Balaban, Neriman

doi:10.1080/713828214

Cited by 26 publications

(30 citation statements)

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“…diff erent population, manual or automated version of TAS Randox ® kit, seminal plasma viscosity. In spite of diff erences in reference values, lower seminal plasma antioxidant capacity was found in human semen with oligoasthenospermia 16 or with astheno-and asthenoteratospermia 18 . Also our study showed signifi cantly lower (p < 0.02) TAS levels in seminal plasma in the study group of men from infertile couples with a prevalence of pathological spermiogram.…”

Section: Discussionmentioning

confidence: 94%

“…One Italian group 16,17 using TAS Randox ® reported in 25 controls mean seminal plasma TAS levels of 1.21 ± 0.16 mmol/l and in another 18 control subjects median TAS 1.19 with a range from 1.0 to 1.4 mmol/l, resp. Higher mean TAS level of 2.02 ± 0.16 mmol/l has been described in 20 fertile Turkish men 18 . Neither of the above papers specifi ed details of their procedure.…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant capacity of seminal plasma measured by TAS Randox®

Fingerová¹,

Novotný²,

Barborik³

et al. 2007

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background: An imbalance between reactive oxygen species (ROS) generation in sperm and antioxidant capacity of seminal plasma has been linked to male infertility. The antioxidant power of biological fl uids can be evaluated either by measurement of individual antioxidants or total antioxidant capacity (TAC). The aim of this study was to assess whether TAS Randox ® can also be used for seminal plasma antioxidant capacity estimation.. Material and Methods:Freshly thawed aliquots of seminal plasma and blood serum of 38 males from infertile couples and 24 healthy normospermic controls were simultaneously assayed using TAS Randox ® reagents on the Hitachi Modular P800 Analyzer. Semen analysis was performed according to WHO guidelines. ROS in fresh sperm suspension in phosphate buff ered saline was measured by chemiluminescence immediately after separation of seminal plasma.Results: Semen analysis showed that in our study group only 14 males were normospermic and 24 males had mostly combined pathologies. The medians for ROS production were similar in both the study and the control groups (4850 and 5450 RLU/min, resp). Seminal plasma TAS levels were signifi cantly lower (p < 0.02) in the study group while blood serum TAS levels were similar in both groups. A signifi cant positive correlation (p < 0.05) between TAS in seminal plasma and serum was found, seminal plasma levels being on average 1.4 times higher.Conclusions: The TAS Randox ® kit may be used for clinical studies intended to identify decreased antioxidant power in the seminal fl uid of infertile men.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Antioxidant capacity of seminal plasma measured by TAS Randox®

Fingerová¹,

Novotný²,

Barborik³

et al. 2007

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…Therefore, they suggested that decreased seminal TAC could play a key role in the etiology of impaired sperm functions (26). Koca et al suggested that antioxidant capacity is positively related to sperm motility and that decreased antioxidant capacity could impair sperm function due to increased ROS production or insufficient antioxidant capacity (27). Appasamy et al observed a negative correlation between antioxidant activity and sperm concentration, which suggests that oxidative stress could influence sperm concentration (28).…”

Section: Discussionmentioning

confidence: 99%

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Fazeli¹,

Salimi²

2016

Avicenna J Med Biochem

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Background: Oxidative stress is the result of an imbalance between the production and scavenging of reactive oxygen species (ROS). Recently, oxidative stress has been introduced as a major cause of male infertility. Objectives: The aim of the present study was to determine the correlation between total antioxidant capacity (TAC) and malondialdehyde (MDA) as markers of oxidative stress in relation to idiopathic male infertility and sperm parameters. Patients and Methods: This case control study was conducted using 35 men with idiopathic infertility and 34 men with proven fertility. Seminal plasma TAC and MDA were measured by ferric reducing ability of plasma (FRAP) and thiobarbituric acid (TBA) reaction methods, respectively. Results: Seminal TAC levels were significantly lower and seminal MDA levels were significantly higher in men with idiopathic infertility than in fertile men (P < 0.0001 and P = 0.004, respectively). A positive correlation was shown between sperm motility, sperm morphology, and TAC levels in men with idiopathic infertility (P = 0.002 and P = 0.002, respectively). In addition, there was a correlation between sperm motility and TAC levels in fertile men (P = 0.005). There was no correlation between sperm count and TAC levels in either men with idiopathic infertility or in fertile men. Negative correlations were observed between MDA levels and sperm motility, morphology, and sperm count only in men with idiopathic infertility (P = 0.003, P = 0.001, and P = 0.006, respectively). Conclusions: Our results show that oxidative stress could play an important role in male infertility as well as in sperm motility and sperm morphology.

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“…Koca et al, showed that the seminal plasma TAC in infertile asthenozoospermic and asthenoteratozoospermic males was lower than that in fertile men [27].…”

Section: Discussionmentioning

confidence: 99%

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

Pahune

Choudhari²,

Muley³

2013

JCDR

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Background: There are growing evidences that the damage which is caused to the spermatozoa by the Reactive Oxygen Species (ROS) plays a key role in the male infertility. The seminal plasma is endowed with many enzymatic and nonenzymatic antioxidants which protect the spermatozoa against oxidative stress.The present study was undertaken by using a simple, colourimetric, ferric reducing, antioxidant power for assessing the total antioxidant power rather than the individual antioxidants. The measurement of the individual antioxidants in the seminal plasma, such as Superoxide Dismutase, Vitamin E, etc. is time consuming, which often requires sophisticated and expensive techniques and these measurements may not correlate with the quality of semen. Aim:To evaluate the total antioxidant capacity of seminal plasma by estimating the Ferric Reducing Antioxidant Power (FRAP) of semen in different groups of subjects and to correlate it with the different seminogram parameters. Material and Methods:The semen samples were obtained from 150 male partners of infertile couples who attended the Reproductive Biology Unit (Infertility Clinic) of the Department of Physiology, MGIMS, Sevagram, who were aged 20-58 years and they were analyzed for the routine seminogram parameters. All the subjects were categorized into two main groups, A. The subjects with abnormal ejaculates, who were further sub classified into the following groups i) Asthenoteratozoospermics (n=25) ii) Oligoasthenoteratozoospermics (n=26) and iii) Azoospermics (n=19) and B. The subjects with normal ejacu lates (n=80). The total antioxidant power was measured spectro photometrically by using the Ferric Reducing Antioxidant Power (FRAP) assay. Results:The Total Antioxidant Capacity (TAC) was found to be significantly lower in the abnormal ejaculates than in the normal ejaculates. A statistically significant positive correlation was observed between the TAC and all the seminogram parameters such as the sperm concentration, sperm motility and sperm morphology (p<0.05). Conclusion:A decreased seminal plasma antioxidant capacity (TAC) could have significant role in the aetiology of impaired sperm functions. So, the TAC may be used as specific biomarker for assessing the oxidative stress in sperms.

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Antioxidant Activity of Seminal Plasma in Fertile and Infertile Men

Cited by 26 publications

References 0 publications

Antioxidant capacity of seminal plasma measured by TAS Randox®

Antioxidant capacity of seminal plasma measured by TAS Randox®

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

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