Antioxidant activity of Terminalia arjuna bark extract on N-nitrosodiethylamine induced hepatocellular carcinoma in rats

Abstract

The present investigation was carried out to evaluate the antioxidant nature of ethanolic extract of Terminalia arjuna bark (EETA) on N-nitrosodiethylamine (DEN) induced liver cancer in male Wistar albino rats. Liver cancer was induced by single intraperitonial injection of DEN (200 mg/kg). After 2 weeks of DEN administration, Phenobarbital (PB) was given to promote the cancer for up to 14 successive weeks. EETA extract (400 mg/kg) was given post-orally for 28 days to hepatocellular carcinoma-bearing rats. After the experimental period, all the animals were sacrificed and serum, liver and kidney samples were collected for further biochemical analysis. The levels of lipid peroxides (LPO) under basal and also in the presence of inducers (H(2)O(2), ascorbate and FeSO(4)) were estimated in serum, liver and kidney of control and experimental animals. Enzymic antioxidants, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and non-enzymic antioxidants like Vitamin C (Vit-C) and Vitamin E (Vit-E) levels were determined in all the groups of animals. A significant increase in LPO levels were observed while the levels of enzymic and non-enzymic antioxidants were decreased, when subjected to DEN induction. These altered enzyme levels were ameliorated significantly by administration of EETA at the concentration of 400 mg/kg in drug-treated animals. This protective effect of EETA was associated with inhibition of LPO induced by DEN and to maintain the antioxidant enzyme levels. Our results show an antioxidant activity of T. arjuna bark against DEN-induced liver cancer.

Protective action of indole‐3‐acetic acid on induced hepatocarcinoma in mice

“…There are reports which have showed that a single necrogenic DEN dose in rats decreased SOD and CAT activities after 7, 14, and 21 days. 40 In our study, DEN exposure showed a drastic alteration in liver morphology and serum ALT and AST activities, similar to detection by others studies. 34 In this work, IAA was shown to provide efficient protection on hepatic CAT and GR activities to the DEN effect and this auxin promoted a partial protection on the down-regulation of gene expression of antioxidant enzymes induced by DEN exposition.…”

“…Hence, 4-ethyldeoxythymidine adduct accumulates in hepatocyte DNA following DEN administration which is thought to be important in tumor initiation. 19 There is extensive evidence that ROS participate in DENinduced hepatocarcinogenesis. 20 Recent work shows that indole compounds have been studied in vivo and in vitro as antitumor and chemopreventive agents, 4 but no studies have been reported on the protective effects of IAA on induced carcinogenesis.…”

Chemo Preventive Effect of Rutin Against N-Nitrosodiethylamine-Induced and Phenobarbital- Promoted Hepatocellular Carcinoma in Wistar Rats

“…20 The activity of Ca 2+ ATPase was assayed Group II-Disease control Received DEN at a single dose of 200 mg/kg (body weight) intraperitoneally on 1 st week followed by administration of phenobarbital (PB) (0.05% w/v) daily after 2 nd week through drinking water up to 16 successive weeks. 13,14 Group III-Treatment group Received DEN at a single dose of 200 mg/kg (body weight) intraperitoneally on 1 st week followed by administration of Phenobarbital (PB) (0.05% w/v) daily after 2 nd week through drinking water up to 16 weeks. Rutin was given orally after 2 nd week at a dose of 50 mg/kg, 15,16 (body weight) up to 16 successive weeks.…”

Chemopreventive Effect of Acanthus ilicifolius Extract on Modulating Antioxidants, Lipid Peroxidation and Membrane Bound Enzymes in Diethyl Nitrosamine Induced Liver Carcinogenesis

“…After A. ilicifolius extract treatment, the alterations in the levels of these enzymes were reverted back to near normal, which may be due to inhibition of glycolytic pathway and activation of gluconeogenesis. Thus, the plant extract may interrupt the energy requirement of tumor tissues and lead to the suppression of tumor growth (Sivalokanathan et al, 2005).…”