Antioxidant and Anti-Inflammatory activity of Chlorogenic Acid on Lead-Induced Fibroblast Cells

Girsang, Ermi; Lister, I Nyoman Ehrich; Ginting, Chrismis N.; Nasution, Sri Lestari Ramadhani; Suhartina, S.; Munshy, Ubaydillah Zedd; Rizal, R; Widowati, Wahyu

doi:10.1088/1742-6596/1374/1/012006

Cited by 14 publications

(18 citation statements)

References 18 publications

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“…2 and 3). The previous study about CA also stating the reducing ROS level induced by lead (Pb) and hydrogen peroxide (H 2 O 2 ) effect by this substances (Girsang et al, 2019c;Hoelzl et al, 2010), CA has antiaging property by inhibiting enzyme related skin aging i.e., MMP-1 and human skin fibroblast elastase (HSFE) according in silico analysis (Girsang et al, 2019b).…”

Section: Discussionmentioning

confidence: 98%

“…UV-induced aging mechanism triggered free radicals in the form of increased intracellular ROS level, leading to oxidative stress, which then triggered inflammation leading to cell death (apoptosis). BJ fibroblast cells are the model of aging cells induced by UV (Girsang et al, 2019c). Fibroblast cells play a role in tissue granulation and scar formation during the inflammation process.…”

Section: Discussionmentioning

confidence: 99%

“…The cells with 80% confluency (n = 10 6 in six-well plate) were incubated with UV exposure for 75 min (37 C, 5% CO 2 ). The cells induced from UV exposure were treated for 4 days with CA (BP0345; Chengdu Biopurify Phytochemical) and were harvested with 0.25% trypsin-EDTA (25200072; Gibco, Waltham, MA, USA) after 4 days treatment (Widowati et al, 2016;Girsang et al, 2019c).…”

Section: Methodsmentioning

confidence: 99%

“…They were then stained with 25 µM DCFDA and incubated for 30 min in 37 C, with 5% CO 2 . The result was analyzed using MACSQuant Analyzer 10 Flow Cytometer (Miltenyi, Bergisch Gladbach, Germany) (Widowati et al, 2014;Niocel et al, 2019;Girsang et al, 2019c;Lister et al, 2020).…”

Section: Measurement Of Ros Levelmentioning

confidence: 99%

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Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Girsang

Ginting

Lister

et al. 2021

PeerJ

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Background Skin aging is the most common dermatological problem caused by intrinsic and extrinsic factor, such as exposure to (ultraviolet) UV rays. Chlorogenic acid (CA) is a phenolic compound which is known for its antioxidant properties against oxidative stress. Objective This study investigates the antiaging and anti-inflammatory properties of CA on UV-induced skin fibroblast cells. Methods Anti-inflammatory properties of CA were assessed by measuring inflammatory-related proteins IL-1β and TNF-α, while antiaging properties of CA were assessed by measuring reactive oxygen species (ROS), apoptosis, live and necrotic cells, and COL-3 gene expression level. Results Treating UV-induced skin fibroblast cells with CA decreased the level of ROS, IL-1β, TNF-α, apoptotic cells, and necrotic cells and increased live cells and COL-3 gene expression. Conclusion CA has the potential as the protective compound against inflammation and aging by decreasing the level ROS, pro-inflammatory cytokines IL-1β and TNF-α, apoptotic cells, and necrotic cells and by increasing live cells and COL-3 gene expression.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Measurement Of Ros Levelmentioning

confidence: 99%

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Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Girsang

Ginting

Lister

et al. 2021

PeerJ

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“…Based on Prahastuti et al. (2019) and Girsang et al. (2019) , the 2′,7′–dichlorofluorescin diacetate (DCFDA)–Cellular Reactive Oxygen Species Detection Assay Kit (Abcam, ab113851) was used to measured intracellular ROS with modifications.…”

Section: Methodsmentioning

confidence: 99%

Hepatotoxicity prevention in Acetaminophen-induced HepG2 cells by red betel (Piper crocatum Ruiz and Pav) extract from Indonesia via antioxidant, anti-inflammatory, and anti-necrotic

Ginting

Lister

Girsang

et al. 2021

Heliyon

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Chlorogenic acid in preventing and curing ultraviolet-induced damage in human skin fibroblast as an antiaging cell model

et al. 2023

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Continuous ultraviolet (UV) irradiation stimulates the over-production of reactive oxygen species (ROS) to cause degenerative diseases. Chlorogenic acid (CA) is found as plants antioxidant that promises medicinal effects. This study examined CA protection against UV-damage in human skin fibroblast (BJ) cells both for curative and preventive therapy. BJ cells were exposed to UV radiation and the addition of CA (6.26-100 μg/mL) by preventive and curative addition methods. The cells viability analysis was conducted employing MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. CA treatment before UV exposure exhibited an increased percentage of viability cells than the positive control. In detail, the series of CA concentration (6.25, 12.5, and 25 μg/mL) significantly enhanced the percentage of viable cells. The addition of CA after UV exposure denoted the same results. Furthermore, the lower CA concentrations used, the higher cell viability resulted. CA at dose 6.25 μg/mL showed the highest viability in cells, while CA 100 μg/mL resulted in the lowest viability. In short, CA can preserve and treat cells from UV exposure. The outcome suggested prevention and curative on UV-induced BJ cells, and the tested concentration is applicable for further experiments.

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Antioxidant and Anti-Inflammatory activity of Chlorogenic Acid on Lead-Induced Fibroblast Cells

Cited by 14 publications

References 18 publications

Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Hepatotoxicity prevention in Acetaminophen-induced HepG2 cells by red betel (Piper crocatum Ruiz and Pav) extract from Indonesia via antioxidant, anti-inflammatory, and anti-necrotic

Chlorogenic acid in preventing and curing ultraviolet-induced damage in human skin fibroblast as an antiaging cell model

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