Antioxidant and anti-inflammatory potential of pomegranate rind extract to ameliorate cisplatin-induced acute kidney injury

Abstract

Cisplatin is a chemotherapeutic agent, but the therapeutic utility is limited due to its dose dependent nephrotoxicity. The aim of the present study was to evaluate the nephroprotective effect of pomegranate in cisplatin-induced acute kidney injury. Wistar rats were allocated into six groups as follows: the normal control, cisplatin-induced, pomegranate rind extract treatment (50, 100 and 200 mg kg(-1)) and pomegranate rind extract per se group. All the experimental test drugs/vehicle were administered orally for a period of ten days. Intraperitoneal injection of cisplatin (8 mg kg(-1)) was administered on day 7 to all the groups except the normal control and pomegranate per se group. On day 10, cisplatin resulted in significant nephrotoxicity in Wistar rats with a drastic elevation of serum creatinine and BUN, a decline in the concentrations of GSH, MDA and superoxide dismutase (SOD), and an elevation in the TNF-α level in renal tissues. Pathological changes in renal tissues were examined by histopathology and dysfunction in mitochondria and proximal tubule cells was detected by transmission electron microscopy. The rate of apoptosis and the expression of caspase-3, Il-1β and IL-6 in rat renal tissues were detected by immunohistochemistry. The administration of pomegranate at a dose of 200 mg per kg body weight significantly (p < 0.001) ameliorates increased serum creatinine and BUN. In parallel to this, pomegranate also exhibits anti-apoptotic activity through the reduction of active caspase-3 expression in kidneys. Additionally, in-silico studies also confirmed a renoprotective effect of pomegranate. The above findings suggest that pomegranate can be used as a dietary supplement in the treatment of cisplatin-induced kidney injury by reducing apoptosis, oxidative stress and inflammation.

Pomegranate supplementation attenuates inflammation, joint dysfunction via inhibition of NF‐κB signaling pathway in experimental models of rheumatoid arthritis

“…Earlier studies on punicalagin and punicalin isolated from Terminalia catappa on carrageenan‐induced paw edema assay in rats also revealed significant anti‐inflammatory activity (Lin et al, ). An in‐silico docking study on various constituents of pomegranate was conducted by us, on different pro‐inflammatory cytokines (IL‐1, IL‐6, TNF‐α) and apoptotic biomarkers (caspase‐3, Bcl‐2, Bax) and concluded that punicalagin possess lowest binding energy, thus might be responsible for anti‐inflammatory and anti‐apoptotic activities in pomegranate rind extract (Karwasra et al, ).…”

“…concluded that punicalagin possess lowest binding energy, thus might be responsible for anti-inflammatory and anti-apoptotic activities in pomegranate rind extract (Karwasra et al, 2016).…”

“…It has been shown to have significant topical anti‐inflammatory (Lee, Chen, Liang, & Wang, ; Mo, Panichayupakaranant, Kaewnopparat, Songkro, & Reanmongkol, ), antibacterial (Dey et al, ), wound‐healing (Hayouni et al, ), anti‐infective (Voravuthikunchai et al, ), anti‐cancer (Kawaii & Lansky, ), diuretic, and (Satomi et al, ) antimutagenic properties (Negi, Jayaprakasha, & Jena, ). In addition to this, pomegranate rind (peels) are also endowed with antioxidant (Li et al, ), nephroprotective (Karwasra et al, ) and as anti‐fibrotic agent (Toklu et al, ). It has been studied by various investigators that the protective effect offered by pomegranate has been exhibited to its ability to suppress the level of reactive oxygen species (Chidambara Murthy et al, ).…”

“…In addition to this, pomegranate rind (peels) are also endowed with antioxidant (Li et al, 2006), nephroprotective (Karwasra et al, 2016) and as anti-fibrotic agent (Toklu et al, 2007). It has been studied by various investigators that the protective effect offered by pomegranate has been exhibited to its ability to suppress the level of reactive oxygen species (Chidambara Murthy et al, 2002).…”

Phytotherapy of nephrotoxicity-induced by cancer drugs: an updated review

“…The administration of pomegranate rind extract significantly ameliorated increased serum creatinine and BUN, which were induced by cisplatin. In addition, pomegranate exhibited antiapoptotic activity due to the decreased activity of caspase-3 expression ( 24 ).…”