Antioxidant and anti-tumor activity of a polysaccharide from freshwater clam, Corbicula fluminea

Liao, Ningbo; Chen, Shiguo; Ye, Xingqian; Zhong, Jianjun; Wu, Nian; Dong, Shengyi; Yang, Bo; Liu, Donghong

doi:10.1039/c2fo30178d

Cited by 54 publications

(37 citation statements)

References 34 publications

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“…We previously reported that a C . fluminea sulfate polysaccharide, CFPS-2, displays marked inhibitory effects on the growth of SKOV3 human ovarian carcinoma cells and SGC7901 human gastric cancer cells [2]. Zhu et al reported that another bioactive glycoprotein, CFp-a, from C .…”

Section: Introductionmentioning

confidence: 99%

Protein-Bound Polysaccharide from Corbicula fluminea Inhibits Cell Growth in MCF-7 and MDA-MB-231 Human Breast Cancer Cells

Liao

Zhong

et al. 2016

PLoS ONE

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A novel protein-bound polysaccharide, CFPS-1, isolated from Corbicula fluminea, is composed predominantly of mannose (Man) and glucose (Glc) in a molar ratio of 3.1:12.7. The polysaccharide, with an average molecular weight of about 283 kDa, also contains 10.8% protein. Atomic force microscopy, high-performance liquid chromatography, Fourier transform infrared spectroscopy, gas chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy analyses revealed that CFPS-1 has a backbone of 1,6-linked and 1,4,6-linked-α-D-Glc, which is terminated with a 1-linked-α-D-Man residue at the O-4 position of 1,4,6-linked-α-D-Glc, in a molar ratio of 3:1:1. Preliminary in vitro bioactivity tests revealed that CFPS-1 effectively and dose-dependently inhibits human breast cancer MCF-7 and MDA-MB-231 cell growth, with an IC50 of 243 ± 6.79 and 1142 ± 14.84 μg/mL, respectively. In MCF-7, CFPS-1 produced a significant up-regulation of p53, p21, Bax and cleaved caspase-7 and down-regulation of Cdk4, cyclin D1, Bcl-2 and caspase-7. These effects resulted in cell cycle blockade at the S-phase and apoptosis induction. In contrast, in MDA-MB-231, with limited degree of change in cell cycle distribution, CFPS-1 increases the proportion of cells in apoptotic sub-G1 phase executed by down-regulation of Bcl-2 and caspase-7 and up-regulation of Bax and cleaved caspase-7. This study extends our understanding of the anticancer mechanism of C. fluminea protein-bound polysaccharide.

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Section: Introductionmentioning

confidence: 99%

Protein-Bound Polysaccharide from Corbicula fluminea Inhibits Cell Growth in MCF-7 and MDA-MB-231 Human Breast Cancer Cells

Liao

Zhong

et al. 2016

PLoS ONE

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“…5-year survival after application of chemotherapy at patients with GC resectable is less than 20% and therefore the effectiveness of chemotherapy is disputed [14]. 5-year survival of the four groups classified by differentiation and depth of penetration in layers is [T2/3 (differentiated type), T2/3 (undifferentiated type), T4a (differentiated type), and T4a (undifferentiated type)] were 98%, 92%, 80%, and 72%, respectively (P < 0.01) [15]. Inhibitory effects on cell Ca growth of the stomach have antioxidant substances like: CFPS-2 containing: Glucosamin, glucose, Galactose, Fructose, protein and sulfate groups [16].…”

Section: Discussionmentioning

confidence: 99%

Presentation of signet ring cell type at carcinoma ventriculi of the patient aged 20 years old

Avdaj¹,

Gözalan²,

Hyseni³

et al. 2013

CRCM

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Introduction: Diffuse variant of GC is composed of gastric-type mucous cells, which generally do not form glands, but rather permit the mucosa and wall as scattered individual cells or small clusters in an "infiltrative" growth pattern. These cells appear to arise from the middle layer of the mucosa, and the presence of intestinal metaplasia is not a prerequisite. In this version, mucin formation expands the malignant cells and pushes the nucleus to the periphery, creating a "signet ring" conformation. If the signetring cells are more than 50% of the tumor, the tumor is classified as signetring cell carcinoma [1]. This case is important for reporting because we encountered for the first time such a carcinoma type, due to the new age and its atypical presentation. Case Presentation: We report a case of a 20 years Albanian old patient with Signet Ring Cell Type of Gastric CA. The patient was brought at the urgency with severe abdominal pain, nausea and peritoneal irritation. Clinical examination has been made in emergency, where we conclude the signs of peritoneal irritation, from native Ro no signs of pneumoperitoneum, while laboratory tests found a slight anemia (erythrocytes 3.36, HCT 25, HGB 8.6). Two hours later we repeated the native RTG and there were present the signs of pneumoperitoneum. It was indicated urgent surgical intervention. Intraoperatively, we found Ulcer duodenal perforation and undertook the operation procedures by Roscoe Graham technique. Conclusions: At this age, it is rare, and it is difficult to detect in its early stages, because the signs and symptoms are often non-existent, non-specific, or mimic as an ulcer. The most common symptoms are early heartburn indigestion, abdominal pain or discomfort, vomiting, constipation, diarrhea or to feel of filling after a small meal, loss of appetite, weakness and fatigue. Less common symptoms are anemia and weight loss.

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“…[21][22][23] PS or PSP can be explored as a functional food or medicine for potential applications in food industry, cosmetics, and pharmacy. However, PS or PSP have yet to be separated and characterized from C. uminea through TPP.…”

Section: -20mentioning

confidence: 99%

“…6a-c and Table 1 Additionally, PSP and E-PSP were different from the previous studies because perhaps of the raw material sources and separation protocols. 21,22 In this work, HPGPC was used to determine the molecular weights (MWs) and molecular weight distribution of PSP and E-PSP, and the results are summarized in Table 1. Both PSP and E-PSP were found to be composed of high-MW fractions (2113.4 kDa and 1980.9 kDa) and low-MW fractions (61.5 kDa and 248.4 kDa).…”

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confidence: 99%

Extraction and characterization of anti-oxidative polysaccharide–protein complexes from Corbicula fluminea through three-phase partitioning

et al. 2017

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In this study, a simple, efficient, and green bioseparation technology called three-phase partitioning (TPP) was employed to extract and separate polysaccharide-protein complexes (PSP) from Corbicula fluminea.The highest extraction yield of PSP was 9.0% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.5 : 1.0 (v/v) t-butanol to crude extract ratio, 30 min, and 35 C. This value was higher than the yield (7.6%) of E-PSP prepared through conventional enzyme extraction. The carbohydrate content (81.7%) of PSP was higher and its molecular weight (61.5 kDa; 72.4%) was lower than those of E-PSP.Although PSP and E-PSP are proteoglycans with different monosaccharide and amino acid compositions, their primary chemical structures did not remarkably differ after TPP was performed. The antioxidant activities of PSP were more evident than those of E-PSP in vitro. Thus, TPP could be potentially used for separation of active PS or PSP from natural sources.

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Antioxidant and anti-tumor activity of a polysaccharide from freshwater clam, Corbicula fluminea

Cited by 54 publications

References 34 publications

Protein-Bound Polysaccharide from Corbicula fluminea Inhibits Cell Growth in MCF-7 and MDA-MB-231 Human Breast Cancer Cells

Protein-Bound Polysaccharide from Corbicula fluminea Inhibits Cell Growth in MCF-7 and MDA-MB-231 Human Breast Cancer Cells

Presentation of signet ring cell type at carcinoma ventriculi of the patient aged 20 years old

Extraction and characterization of anti-oxidative polysaccharide–protein complexes from Corbicula fluminea through three-phase partitioning

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