Protein-Bound Polysaccharide from Corbicula fluminea Inhibits Cell Growth in MCF-7 and MDA-MB-231 Human Breast Cancer Cells

Abstract

A novel protein-bound polysaccharide, CFPS-1, isolated from Corbicula fluminea, is composed predominantly of mannose (Man) and glucose (Glc) in a molar ratio of 3.1:12.7. The polysaccharide, with an average molecular weight of about 283 kDa, also contains 10.8% protein. Atomic force microscopy, high-performance liquid chromatography, Fourier transform infrared spectroscopy, gas chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy analyses revealed that CFPS-1 has a backbone of 1,6-linked and 1,4,6-linked-α-D-Glc, which is terminated with a 1-linked-α-D-Man residue at the O-4 position of 1,4,6-linked-α-D-Glc, in a molar ratio of 3:1:1. Preliminary in vitro bioactivity tests revealed that CFPS-1 effectively and dose-dependently inhibits human breast cancer MCF-7 and MDA-MB-231 cell growth, with an IC50 of 243 ± 6.79 and 1142 ± 14.84 μg/mL, respectively. In MCF-7, CFPS-1 produced a significant up-regulation of p53, p21, Bax and cleaved caspase-7 and down-regulation of Cdk4, cyclin D1, Bcl-2 and caspase-7. These effects resulted in cell cycle blockade at the S-phase and apoptosis induction. In contrast, in MDA-MB-231, with limited degree of change in cell cycle distribution, CFPS-1 increases the proportion of cells in apoptotic sub-G1 phase executed by down-regulation of Bcl-2 and caspase-7 and up-regulation of Bax and cleaved caspase-7. This study extends our understanding of the anticancer mechanism of C. fluminea protein-bound polysaccharide.

A Polysaccharide from Amusium Pleuronectes Combined with Praziquantel Treatment Ameliorates Hepatic Fibrosis in Schistosoma Japonicum-Infected Mice

“…For example, a mytilan isolated from the mantle of the mussel Crenomytilus grayanus has high immunomodulating activity [ 11 ]. In addition, a polysaccharide derived from Corbicula fluminea was found to have antioxidant and anti-tumor activity [ 12 , 13 ]. A polysaccharide derived from Cyclina sinensis was investigated and had antioxidant, anti-tumor, and hepatoprotective activities [ 14 , 15 ].…”

Effects of preparation method on the biochemical characterization and cytotoxic activity of New Zealand surf clam extracts

“…Dolastatin 10, a peptide isolated from the shell-less mollusc, Dolabela auricularia , has been shown to bind to tubulin and block cancer cell cycle in the G2/M phase [ 36 ], and this may be the major cause of reduced viability and cellular growth inhibition of the cancer cell lines studied herein. The cell cycle arrest in the G2/M- and S- phases of other molluscs have also been reported [ 35 , 37 , 38 ].…”

Anticancer effect of purified polysaccharide from marine clam Donax variabilis on A549 cells

“…The CFPS-1 with glycosyl residues are connected by α-configuration of glycosidic bonds, and the repeating unit of the CFPS-1 has been described by NMR studies (Liao et al, 2016).…”

“…Protein‐bound polysaccharide from Corbicula fluminea consists of glucose and mannose in a molar ratio of 12.7:3.1 with a molecular weight of about 283 kDa. The CFPS‐1 with glycosyl residues are connected by α‐configuration of glycosidic bonds, and the repeating unit of the CFPS‐1 has been described by NMR studies (Liao et al., 2016).…”

“…GAGs from D. faba could be used as an alternative source of heparin (Periyasamy et al, 2013). CFPS-1, a polysaccharide bounded with protein with a molecular weight of 283 kDa obtained from Corbicula fluminea revealed anticancer activity against breast cancer cells (Liao et al, 2016). Bullacta exarata is another shell mollusk and it is consumed as a food and used in traditional Chinese medicine with the diseases related to liver and kidney (Liao et al, 2017).…”