Antioxidant And Anticancer Activities Of Murbei (Morus Alba L.) Stem Extract On In Vitro WiDr Cancer Cells

Abstract

Mulberry is considered as an important plant in traditional Chinese medicine, due to its various compounds, including phenols and flavonoids. These flavonoids have antioxidant activities so that can be a potential anticancer candidate. The aims of this study were to determining antioxidant activity, phenolic content, and potential anticancer activity in Mulberry stem extract. The extraction was carried out by maceration using ethanol as the solvent, antioxidant activity test using ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) method, phenolic content determination using Folin-Ciocalteu reagents, and anticancer activity test using the MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolium bromida) method on WiDr cancer cells and Vero cells. The result of total phenolic Mulberry stem extract was 35.9%, the antioxidant activity value was 83.18 µg / mL, the IC50 value for anticancer activity for WiDr cells was 71.24 µg / mL and Vero cells IC50 value was 154.241 µg / mL. It could be concluded that the Mulberry stem ethanol extract had strong antioxidant activity and had the potential anticancer selectively against cancer cells WiDr.

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“…Many bioactive compounds have shown specific role as anti-cancer agents. The presence of polyphenolic compounds 30,31 , tannins 32 , anthraquinone glycosides 33 , cardiac glycosides 34 and terpenoids 35 which specifically are known to possess anticancer properties makes MI hybrid varieties a novel source of nutraceutical compounds. Though certain compounds are present in smaller amounts, they work synergistically and exhibit their biological role.…”

Mulberry plant as a source of functional food with therapeutic and nutritional applications: A review

“…Their study showed a 154.241 μg/ml IC50 value for the anticancer activity for Vero cells, 71.24 μg/ml IC50 value for WiDr cells, and 83.18 μg/ml value for antioxidant activity (Burhan et al, 2019).…”

“…Ethanolic extract from mulberry stem had potent antioxidant and antimutagenic activities. Their study showed a 154.241 μg/ml IC50 value for the anticancer activity for Vero cells, 71.24 μg/ml IC50 value for WiDr cells, and 83.18 μg/ml value for antioxidant activity (Burhan et al, 2019). Guangsangon E, a novel Diels‐Alder‐adduct, isolated from M. alba leaves, was studied in CNE1 and A549 cells.…”

“…The roots have neuroprotective (Xia et al, 2019), antibacterial (Mekvimol et al, 2020), antifungal (Kwon et al, 2019), anti‐inflammatory (Wu et al, 2020), hepatoprotective (Guo, Ren, et al, 2020), and good skin penetrating properties (Nugraha et al, 2020). The plant stem has various therapeutic effects such as anti‐tumor (Burhan et al, 2019), antibacterial (Aulifa et al, 2021), antioxidant (Pham et al, 2017), and hypoglycemic (Basu et al, 2021). Besides pharmacological actions, mulberry supplementation in food products extended shelf life via reducing lipid and protein oxidation and antimicrobial properties (Cheng, Zhu, & Liu, 2020; Xu et al, 2018).…”