Antioxidant and anticancer peptides from the protein hydrolysate of blood clam (Tegillarca granosa) muscle

Chi, Chang-Feng; Hu, Fa-Yuan; Wang, Bin; Tao, Lei; Ding, Guofang

doi:10.1016/j.jff.2015.03.045

Cited by 182 publications

(180 citation statements)

References 58 publications

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“…Peptides having smaller molecular masses are generally believed to have greater antioxidant activity than those having larger masses [25,26]. In accordance with this, during the fractionation of marine invertebrate protein hydrolysates with UF membranes, peptidic fractions having the lowest molecular mass range showed the highest DPPH radical scavenging activity [31,33,36] and the highest hydroxyl radical scavenging activity [42,53]. Similarly, UF fractions with the lowest molecular mass range (<3 kDa), which were prepared from the giant squid muscle [43] and jumbo squid skin gelatin [41], had the highest inhibitory activity against linoleic acid oxidation relative to other UF fractions in the same study.…”

Section: Molecular Characteristics and Structure–activity Relationmentioning

confidence: 93%

“…Following peptide sequencing, mass spectrometry was used by some research groups to determine the molecular masses of the antioxidant peptides identified [31,33,48]. The use of SEC to further purify the antioxidant peptide contained in an active RP-HPLC fraction prior to determining the amino acid sequence of the peptide by using the Edman degradation method was also reported [44,53].…”

Section: Enzyme-assisted Production Purification and Identificatmentioning

confidence: 99%

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Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

et al. 2017

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Marine invertebrates, such as oysters, mussels, clams, scallop, jellyfishes, squids, prawns, sea cucumbers and sea squirts, are consumed as foods. These edible marine invertebrates are sources of potent bioactive peptides. The last two decades have seen a surge of interest in the discovery of antioxidant peptides from edible marine invertebrates. Enzymatic hydrolysis is an efficient strategy commonly used for releasing antioxidant peptides from food proteins. A growing number of antioxidant peptide sequences have been identified from the enzymatic hydrolysates of edible marine invertebrates. Antioxidant peptides have potential applications in food, pharmaceuticals and cosmetics. In this review, we first give a brief overview of the current state of progress of antioxidant peptide research, with special attention to marine antioxidant peptides. We then focus on 22 investigations which identified 32 antioxidant peptides from enzymatic hydrolysates of edible marine invertebrates. Strategies adopted by various research groups in the purification and identification of the antioxidant peptides will be summarized. Structural characteristic of the peptide sequences in relation to their antioxidant activities will be reviewed. Potential applications of the peptide sequences and future research prospects will also be discussed.

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Section: Molecular Characteristics and Structure–activity Relationmentioning

confidence: 93%

Section: Enzyme-assisted Production Purification and Identificatmentioning

confidence: 99%

Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

et al. 2017

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“…Currently, chemotherapy is the most common method used to eliminate cancer cells, prevent cancer recurrence, control cancer by slowing cell growth, and reduce symptoms [2]. However, healthy cells may be damaged by the many side effects of anticancer drugs, and resistance to anticancer drugs has been observed [3].…”

Section: Introductionmentioning

confidence: 99%

Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells

Pan

Zhao

et al. 2016

Marine Drugs

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In this study, the hexapeptide Phe-Ile-Met-Gly-Pro-Tyr (FIMGPY), which has a molecular weight of 726.9 Da, was separated from skate (Raja porosa) cartilage protein hydrolysate using ultrafiltration and chromatographic methods, and its anticancer activity was evaluated in HeLa cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay indicated that FIMGPY exhibited high, dose-dependent anti-proliferation activities in HeLa cells with an IC50 of 4.81 mg/mL. Acridine orange/ethidium bromide (AO/EB) fluorescence staining and flow cytometry methods confirmed that FIMGPY could inhibit HeLa cell proliferation by inducing apoptosis. Western blot assay revealed that the Bax/Bcl-2 ratio and relative intensity of caspase-3 in HeLa cells treated with 7-mg/mL FIMGPY were 2.63 and 1.83, respectively, significantly higher than those of the blank control (p < 0.01). Thus, FIMGPY could induce apoptosis by upregulating the Bax/Bcl-2 ratio and caspase-3 activation. Using a DNA ladder method further confirmed that the anti-proliferation activity of FIMGPY was attributable to its role in inducing apoptosis. These results suggest that FIMGPY from skate cartilage protein hydrolysate may have applications as functional foods and nutraceuticals for the treatment and prevention of cancer.

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“…The peptide was found to effectively induce the apoptosis of prostate, breast and lung cancer cells, and LC 50 values (the concentration of the anticancer peptide that results in the death of 50% of cancer cells) were 1.21, 1.87 and 2.07 mg/mL for PC-3, A549 and MDA-MB-231 cells, respectively. Chi et al (2015) isolated an anticancer peptide from the protein hydrolysate of blood clam (Tegillarca granosa) muscle, which induced apoptosis of PC-3 cells between 11.22 and 22.78% at the concentrations of 5 and 15 mg/mL.…”

Section: Introductionmentioning

confidence: 99%

Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle (Cuora trifasciata)

Mao

Zhang

et al. 2016

Journal of Microencapsulation

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Chinese three-striped box turtle (Cuora trifasciata), as a freshwater turtle, is used as a tonic food. The purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. The results demonstrated that two fractions T1 and T2 exhibited good inhibition on HepG-2 and MCF-7 cancer cells, with an inhibition of 70.65-89.1%, at 500 μg/mL. Subsequently, three peptides were identified from T1 and T2, including RGVKGPR (T1-1), KLGPKGPR (T1-2), and SSPGPPVH (T2-1). By database search, T2-1 was a completely new peptide; its inhibition activity on MCF-7 cancer cells was the best, up to 70.02% at 500 μg/mL. Then, T1 and T2-1 were nanoencapsulated by chitosan. After nanoencapsulation, the inhibition percentages were 50.23% for the nanoencapsulated T1 on HepG-2 and 46.82% for the encapsulated T2-1 on MCF-7. The release experiment indicated that the encapsulated peptides could be slowly released in simulated gastrointestinal juice.

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Antioxidant and anticancer peptides from the protein hydrolysate of blood clam (Tegillarca granosa) muscle

Cited by 182 publications

References 58 publications

Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells

Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle (Cuora trifasciata)

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