Antioxidant and antiglycation activities of traditional plants and identification of bioactive compounds from extracts of Hordeum vulgare by LC–MS and GC–MS

Abstract

Glycation has been involved in Schiff base reaction lead to hyperglycemia at cellular level. The current study aimed to identify the bioactive compounds from selected folkloric plants for their antiglycation and antioxidant potential. Methanol extracts demonstrated the highest activities, therefore, it was further fractionated using n‐hexane, dichloromethane, ethyl acetate, and methanol solvents to isolate the nonpolar compounds from the Hordeum vulgare. Moreover, n‐hexane and dichloromethane fractions of H. vulgare demonstrated the best antioxidant (61.58% and 62.89%) and antiglycation activities (72.52% and 61.52%) at 2 mg/ml, respectively. Analytical techniques of LC–MS and GC–MS were employed for identification of bioactive compounds; Biochanin A in dichloromethane (DCM) and Vitamin E in n‐hexane fractions. There was a strong correlation between antioxidant and antiglycation activities (r = 0.97 and r = 0.96) of DCM & n‐hexane fractions of H. vulgare. Findings of this study established the role of Biochanin A and Vit E from H. vulgare as potent antiglycation agents. Practical applications The results of this study confirmed the potential role of Black Barley has involved in the inhibition of protein glycation, which can be the potential treatment to reduce the complications of Diabetic Patients. The Black Barley has a rich source of identified compounds Biochanin A and Vitamin E. We can use this plant as a staple food in curing the severity of diabetes. The other practical approach is to use this plant as an ingredient of different food products. The extraction of identified bioactive compounds from the plant will be a good and cheap source of the treatment.

Characterization of the complete chloroplast genome of Hordeum vulgare L. var. trifurcatum with phylogenetic analysis

“…trifurcatum) shows good environmental tolerance, which can be successfully planted in high altitude, low temperature, and high salinity places (Walia et al 2006;El-Esawi et al 2018;Elsawy et al 2018). In addition, the highland barley also showed good antioxidant activity (Asif et al 2020). Thus, Hordeum vulgare L. var.…”

Secondary Metabolites Screening, in Vitro Antioxidant and Antidiabetic Activity of Marine Red Alga Botryocladia Leptopoda (J.Agardh) Kylin

“…It is claimed in the literature that the crude extract is rich in phenolic chemicals, which may contribute to its In vitro antidiabetic action 58 . The recent studies proved that the α-amylase and α-glucosidase inhibitory activity red marine alga-like Bangia fuscopurpurea 59 , Padina tetrastromatica 60 , Gracilaria opuntia 61 and Ceramium rubrum 62 . Additionally, the EtBl had the most significant inhibition of α-amylase and α-glucosidase.…”

Comprehensive Overview: The Effect of Using Different Solvents for Barley Extraction with Its Anti‐Inflammatory and Antioxidant Activity

“…Many flavone glycosides were identified from barley leaves methanol extract, such as apigenin, luteolin, chrysoeriol, and tricin glycosides [37] . 4‐methoxy‐5,7‐dihydroxy isoflavone (Biochanin A) was also identified in Pakistanian barley, besides the existence of α ‐tocopherol (Vitamin E) [38] . Myricetin, quercetin & kaempferol were also identified from the barley sprouts in addition to many phenolic acids such as gallic acid, p‐ hydroxybenzoic acid, protocatechuic acid, vanillic acid [33] .…”

“…[37] 4-methoxy-5,7-dihydroxy isoflavone (Biochanin A) was also identified in Pakistanian barley, besides the existence of α-tocopherol (Vitamin E). [38] Myricetin, quercetin & kaempferol were also identified from the barley sprouts in addition to many phenolic acids such as gallic acid, p-hydroxybenzoic acid, protocatechuic acid, vanillic acid. [33] Ge, X., et al (2021) extracted different colored naked barley grains with methanol.…”

“…[41] Azelaic acid which has a strong anti-inflammatory effect was also identified as one of the main compounds in barely after planting for two weeks. [42] The antioxidant activity of barley extracts was tested using different assays such as DPPH, FRAP, and ORAC and it was found that the methanol extract had the highest antioxidant activity, [33,36,38,[43][44][45][46] The methanol extract of barley showed anti-bacterial activity against different pathogens. [47] Table 1 summarizes the activity, as well as the isolated chemicals, and Figure 2, showed the major structures of identified compounds from the methanol extract.…”