Antioxidant and Antiproliferation Activities of Lemon Verbena (Aloysia citrodora): An In Vitro and In Vivo Study

Abstract

Aloysia citrodora (Verbenaceae) is traditionally used to treat various diseases, including bronchitis, insomnia, anxiety, digestive, and heart problems. In this study, this plant’s antioxidant and anti-proliferation effects were evaluated. In addition to volatiles extraction, different solvent extracts were prepared. The GC-MS, LC-MS analysis and the Foline-Ciocalteu (F-C) method were used to investigate the phytochemical components of the plant. MTT assay was used to measure the antiproliferative ability for each extract. Antioxidant activity was determined using the 2,2-diphenylpicrylhydrazyl (DPPH) assay. In in vivo anti-proliferation experiments, Balb/C mice were inoculated with tumor cells and IP-injected with ethyl acetate extract of A. citrodora. After treatment, a significant reduction in tumor size (57.97%) and undetected tumors (44.44%) were obtained in treated mice, demonstrating the antiproliferative efficacy of the ethyl acetate extract. Besides, ethanol extract revealed the most potent radical scavenging effect. The findings of this study displayed that A. citrodora has promising cytotoxic and antioxidant activities. Still, further testing is required to investigate the extract’s chemical composition to understand its mechanisms of action.

Fabrication and application of functional active packaging material based on carbohydrate biopolymers formulated withLemon verbena/Ferulago angulataextracts for the preservation of raw chicken meat

“…Fifty‐one compounds were specified by GC–MS analysis representing 98.5% of the total content (33 compounds of FAE and 18 compounds of LVE). The main compounds for LVE were 1,8‐cineole (9.10%), iso‐menthone (6.43%), menthol (5.10%), and for FAE were α‐pinene (28.43%), myrcene (4.67%), ß‐Ocimene (20.12%), bornyl acetate (7.92%), and germacrene D (5.63%) that have been reported in several former studies and they are in agreement with the findings of this research (Mumivand et al, 2019; Rashid et al, 2022).…”

“…in several former studies and they are in agreement with the findings of this research (Mumivand et al, 2019;Rashid et al, 2022).…”

Chemical Composition and Antioxidant Properties of Common and Lemon Verbena

“…Literature data on the antioxidant activity of common and lemon verbena are very scarce and only DPPH and ABTS assays have been determined so far [ 20 , 23 , 32 , 34 ]. Although we also included these tests in our study, the recorded DPPH and ABTS values cannot be compared with those obtained by other reports due to the differences in the calculation units.…”

“…Britton), commonly known as lemon verbena, is an annual plant that originates in South America and is cultivated throughout the Mediterranean and North Africa [ 30 ]. It has traditionally been consumed as a herbal tea for the treatment of cold and fever, influenza, colic, diarrhea, spasms asthma, anxiety, insomnia, and indigestion cases [ 31 , 32 ]. Moreover, this plant is popularly used as a tincture or essential oil for dermal disorders [ 33 ].…”

Disclosing the potential of Cupressus leylandii A.B. Jacks & Dallim, Eucalyptus globulus Labill., Aloysia citrodora Paláu, and Melissa officinalis L. hydrosols as eco-friendly antimicrobial agents

“…M. officinalis has a phytochemical composition rich in bioactive compounds with pharmacological effects, including antioxidant, antimicrobial, and cytotoxic activities [ 5 ]. Aloysia citrodora , a species of the Verbenaceae family, often used for medical, cosmetic, and aromatic purposes, has remarkable biological properties like antioxidant, antimicrobial, and antiproliferative activities [ 6 ]. In another study [ 7 ], the plant genus Eucalyptus , which belongs to the Myrtaceae family, is referred to as a valuable source of bioactive components with antioxidant and antimicrobial capacity, making it a useful natural preservative for the pharmaceutical, cosmetical and food applications.…”