Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice

Abstract

The present study investigates the antioxidant and hepatoprotective effects of Capparis spinosa L. and Quercetin in tert-butyl hydroperoxide (t-BHP) induced acute liver damage. Different fractions of C. spinosa were examined for total phenolic content and antioxidant property. Among these fractions, hydroalcoholic extract was used to assess the hepatoprotective effect in tert-butyl hydroperoxide (t-BHP) induced hepatotoxicity model by determining serum biochemical markers, sleeping time and antioxidant assay such as reduced glutathione (GSH) as well as histopathological examination of liver tissues. The total phenolic and Quercetin contents of hydroalcoholic fraction were significantly higher than other fractions. It also showed high antioxidant activity. Pretreatment with hydroalcoholic fraction at the dose of 400 mg/kg and Quercetin at the dose of 20 mg/kg showed liver protection against t-BHP induced hepatic injury, as it was evident by a significant decrease in serum enzymes marker, sleeping time and MDA and an increase in the GSH, SOD and CAT activities confirmed by pathology tests. The final results ascertained the hepatoprotective and antioxidant effects of C. spinosa and Quercetin in a dose-dependent manner. Moreover, this study suggests that possible mechanism of this protection may be associated with its property of scavenging free radicals which may be due to the presence of phenolic compounds.

Assessment of antiproliferative activity of Capparis spinosa L extract against Ehrlich ascites carcinoma in Swiss albino mice

“…Treatment with plant extract, rutin and hesperidin reduced most of the pathological alterations induced by EAC cells in mice , there is no appearance of solid carcinoma of aggregated malignant cells.As for preventive plant extract group the liver tissue showed normal hepatic parenchyma; hepatocytes, blood sinusoids, and Portal tract, While for the therapeutic plant extract group, Liver showing hepatocytes vacuolation with prepheral nucleous, the central vein is permeated with leucocytic cells . Our results showed hepatoprotective effect of C. spinosa extract were supported by the study that revealed histopathological studies under light microscope confirms the protective effects of C. spinosa and Quercetin against the t-BHPinduced liver damage [93] .…”

Bioactive properties and phenolic compounds in bud, sprout, and fruit ofCapparisspp. plants

“…Tesoriere et al () reported that caper bud ( C. spinosa ) contained 48.75 mg GAE/100 g. Germanò et al () determined that caper buds have number of flavonoids and other polyphenols. Total phenol contents of hydro‐alcoholic, ethyl acetate, and chloroform extracts obtained from Capparis spinosa leaves were established as 26.59 and 13.66 mg/g, respectively (Kalantari et al, ). Kalantari et al () reported that C. spinosa leave extracts contained 7.415 mg/g (hydro‐alcholic extract), 2.897 mg/g (ethyl acetate ectract), and 4.…”

“…Total phenol contents of hydro‐alcoholic, ethyl acetate, and chloroform extracts obtained from Capparis spinosa leaves were established as 26.59 and 13.66 mg/g, respectively (Kalantari et al, ). Kalantari et al () reported that C. spinosa leave extracts contained 7.415 mg/g (hydro‐alcholic extract), 2.897 mg/g (ethyl acetate ectract), and 4. 986 mg/g (chloform extract) flavonoid.…”

“…986 mg/g (chloform extract) flavonoid. Kalantari et al () determined 0.0349, 0.2770, and 0.0392 mg/ml antioxidant values in hydro‐alcholic, ethyl acetate, and chloroform extracts of C. spinosa leaves, respectively. Grimalt, Hernández, Legua, Almansa, and Amorós () reported that total phenol contents of caper fruits changed between 61.5 and 119.2 mg GAE/100 g (fw).…”

Nephroprotective Effect of Hydroalcoholic Extract Allium jesdianum Boiss against Carbon Tetrachloride Induced Nephrotoxicity via Stress Oxidative in Mice

“…Based on growing interest in free radical biology and the efficacy of antioxidants in protection against oxidative stress has posed interest in the use of antioxidants as a tool to prevent or decelerate the progression of oxidative stress. [12][13][14][15][16] Allium jesdianum Boiss (Allium) is the largest and most important plant genus of alliaceae family ( Figure 1) that its extract and isolated compounds possess pharmacological effects. Previous studies have indicated several biological activities for Allium species including antioxidant, antibacterial, antiviral, antiparasitic, anticarcinogenic, antifungal, anti-protozoa and antidiabetic and also effects on the cardiovascular system and treatment of common cold.…”