Antioxidant and toxicity properties of anthocyanin extracted from red flower of four tropical shrubs

Abstract

Abstract. Kuspradini H, Rosiarto AM, Putri AS, Kusuma IW. 2016. Antioxidant and toxicity properties of anthocyanin extracted from red flower of four tropical shrubs. Nusantara . Some of flowers are colored by anthocyanins and make their own shade profile. Anthocyanins are natural colorants which have gained a growing interest due to their extensive range of colors and beneficial health effects. Anthocyanin content, antioxidant and toxicity activity of four red flowers (Bougainvillea glabra, Jatropha integerrima, Melastoma malabathricum and Mussaenda philippica) has been evaluated in this study by pH-differential, DPPH and Brine Shrimp Lethality method, respectively. In this present work, 1% HCl-ethanol and ethanol solvent were used for the extraction. The extractive yield was varied in different extraction system, 5.80 to 13.30% in 1% HCl-ethanol and 6.29 to 8.81% in ethanol solvent. The IC 50 of antioxidant from different extraction systems were also varied, whereas the acidified ethanol extracts showed a highest scavenging activity than the ethanol extracts, 11.67 to 46.38 ppm and 79.08 to 133.35 ppm, respectively. The free radical scavenging action of 1% HCl-ethanol extracts of flower are in the order as J. integerrima > M. malabathricum > M. philippica > B. glabra while in the ethanol extracts are in the order as M. malabathricum > J. integerrima > B. glabra >M. philippica. In the group of 1% HCl-ethanol extracts, Jatropha integerrima Jacq was the richest in anthocyanin content (15.83 mg CGE/100 g DW) and showed the strongest antioxidant activity (11.67 ppm) and not toxic. Therefore, these can be regarded as a potential pigment source for food and natural product applications.

Phytosynthesis of silver nanoparticles from Jatropha integerrima Jacq. flower extract and their possible applications as antibacterial and antioxidant agent

“…Various parts of Jatropha integerrima Jacq. has been employed as a purgative, styptic, and emetic in treating herpes, rheumatism, tumors, toothaches, warts, pruritis, eczema, scabies, and ringworm for centuries ( Kolawole et al, 2017 , Kuspradini et al, 2016 ). The leaves and seeds of the plant have antibacterial activity, while the branches have been proven to have cholinesterase activity, and the latex has anti-cancer activity ( Ali et al, 2017 , Eshilokun et al, 2019 , Horsten et al, 1996 , Sharma and Singh, 2012 ).…”

Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

“…The DPPH assay was performed as described by Kuspradini et al . 13 . Various concentrations of samples of each extract (12.5, 25, 50 and 100 ppm) in 96% ethanol were added with DPPH.…”

Antioxidant and Antibacterial Activity from Three Different Solvents of Nephelium ramboutan-ake Leaves Crude Extract

“…The radical DPPH was used to evaluate the free radical scavenging activity of the crude extracts and standard vitamin C, modified by the method of [12]. All successive extracts, 100, 50, and 25 ppm, were used as the final concentration in dimethyl sulphoxide.…”