Antioxidant, Antiproliferative, and Antiangiogenesis Effects of Polyphenol-Rich Seaweed (Sargassum muticum)

Abstract

In the present study, we evaluated the effect of brown seaweeds Sargassum muticum methanolic extract (SMME), against MCF-7 and MDA-MB-231 breast cancer cell lines proliferation. This algae extract was also evaluated for reducing activity and total polyphenol content. The MTT assay results indicated that the extracts were cytotoxic against breast cancer cell lines in a dose-dependent manner, with IC50 of 22 μg/ml for MCF-7 and 55 μg/ml for MDA-MB-231 cell lines. The percentages of apoptotic MCF-7-treated cells increased from 13% to 67% by increasing the concentration of the SMME. The antiproliferative efficacy of this algal extract was positively correlated with the total polyphenol contents, suggesting a causal link related to extract content of phenolic acids. Cell cycle analysis showed a significant increase in the accumulation of SMME-treated cells at sub-G1 phase, indicating the induction of apoptosis by SMME. Further apoptosis induction was confirmed by Hoechst 33342 and AO/PI staining. Also SMME implanted in vivo into fertilized chicken eggs induced dose-related antiangiogenic activity in the chorioallantoic membrane (CAM). Our results imply a new insight on the novel function of Sargassum muticum polyphenol-rich seaweed in cancer research by induction of apoptosis, antioxidant, and antiangiogenesis effects.

Antiangiogenic, wound healing and antioxidant activity ofCladosporium cladosporioides(Endophytic Fungus) isolated from seaweed (Sargassum wightii)

“…Even though marine endophytes are studied for antioxidant, cytotoxic and pharmacological potential; much information was not available for the antiangiogenic activity. C. cladosporioides extract showed similar results as that of another fungus ( P.citrinum ) isolated from S. weightii as well as the activity of terrestrial and seaweed endophytes reported earlier (Ben et al 2009; Udagawa et al 2000; Namvar et al 2013; Manjunath et al 2016). …”

Algae as potential repository of anti cancerous natural compounds

“…Dalton's Ascitic lymphoma, HepG2, HT-29, Ca co-2, T47D, MDA-MB468 and /NIH 313, [55][56][57][58][59][60] Stypopodium sp SH-SY5Y, RBL-2H3, (Raw267), (V79), Caco-2. DU-145 [53,62] Undaria sp is one of the important genera under Pheophyceae.…”

Sitotoksisitas Ekstrak Aseton Dan Kandungan Fukosantin Rumput Laut Sargassum

“…Ekstrak metanol S. muticum memiliki aktivitas sitotoksik terhadap sel kanker payudara MCF-7 dan MDA-MB-231 dengan nilai IC 50 sebesar 22,0 µg/ml dan 55,0 µg/ml (Namvar et al, 2013). Khanavi et al (2010) memperlihatkan bahwa ekstrak metanol S.swartzii memiliki nilai IC 50 sebesar 205,2 µg/ml terhadap sel T47D sedangkan fraksi n-heksana memiliki nilai IC 50 sebesar 99,9 µg/ml terhadap sel Caco-2.…”