Antioxidant capacity of 3D human skin EpiDerm<sup>TM</sup> model: effects of skin moisturizers

Grazul-Bilska, Anna T.; Bilski, Jerzy J.; Redmer, Dale A.; Reynolds, Lawrence P.; Abdullah, Kay M.; Abdullah, A.

doi:10.1111/j.1468-2494.2009.00499.x

Cited by 9 publications

(6 citation statements)

References 56 publications

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“…The skin consists of an interface between the human body and the environment. Therefore, it is exposed to endogenous and exogenous oxidant agents that could generate many free radicals [1]. Oxidative stress arises when the formation of free radicals is greater than its elimination by the antioxidant defense system of the body.…”

Section: Introductionmentioning

confidence: 99%

Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

et al. 2018

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The goal of this study was to investigate the efficacy and the safety of grape pomace (Vitis labrusca L.) obtained from the winemaking process as an antioxidant raw material for cosmetic formulations. Grape pomace was dried and submitted to extraction with solvents. The extracts were filtered and freeze-dried. The extraction carried out with 75% acetone-water showed a better yield (1.9 g·g−1). High performance liquid chromatography (HPLC) analysis confirmed the presence of ellagic acid as an active biocompound in the extracts. The Total phenolic content of freeze-dried extracts reached 69.83 ± 1.02 mg for 75% acetone-water system. Antioxidant activity determined by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method revealed there was no statistical difference between the extract (EC50 6.9 ± 0.21) and butylated hydroxytoluene (BHT) (EC50 7.6 ± 0.71). Results of cytotoxicity showed that extracts from grape pomace were safe, even at the highest concentration tested (200 mg·mL−1) against the 3T3 cell line. Scanning Electron Microscopy (SEM) analysis revealed that there were no morphological cell changes of cells treated. Cytoprotection of cells towards the oxidation promoted by the peroxide solution was observed in the lowest concentration tested (0.73 mg·mL−1). Grape pomace from the winemaking process revealed a significant antioxidant activity attributed to its biocompounds. The safety and efficacy of extracts of grape pomace as raw material for cosmetic formulations was proposed through cell culture assays.

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Section: Introductionmentioning

confidence: 99%

Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

et al. 2018

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“…For this reason, the stability of antioxidants in the tapes was explored in different conditions for several time frames. A close review of the literature revealed the usage of a variety of storage conditions in skin antioxidant studies, such as −70 °C in a study by Grazul-Bilska et al [ 54 ], 4 °C for biopsy samples in other studies [ 31 , 55 ] and −20 °C for the separated epidermis, biopsies and excised skin in work by others [ 56 , 57 , 58 ]. Furthermore, a temperature of −20 °C was found to be effective in the storage of tapes after tape stripping for penetration studies [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Assessing the Oxidative State of the Skin by Combining Classical Tape Stripping with ORAC Assay

2022

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The antioxidant barrier system of the skin acts as the main defence against environmental pro-oxidants. Impaired skin oxidative state is linked to unhealthy conditions such as skin autoimmune diseases and cancer. Thus, the evaluation of the overall oxidative state of the skin plays a key role in further understanding and prevention of these disorders. This study aims to present a novel ex vivo model to evaluate the skin oxidative state by the measurement of its antioxidant capacity (AOC). For this the ORAC assay was combined with classical tape stripping and infrared densitometry to evaluate the oxidative state of the stratum corneum (SC). Outcomes implied the suitability of the used model to determine the intrinsic antioxidant capacity (iAOC) of the skin. The average iAOC of untreated skin was determined as 140 ± 7.4 µM TE. Skin exposure to UV light for 1 h reduced the iAOC by about 17%, and exposure for 2 h decreased the iAOC by about 30%. Treatment with ascorbic acid (AA) increased the iAOC in a dose-dependent manner and reached an almost two-fold iAOC when 20% AA solution was applied on the skin. The application of coenzyme Q10 resulted in an increase in the iAOC at low doses but decreased the iAOC when doses > 1% were applied on the skin. The results show that the combination of classical tape stripping and ORAC assay is a cost-effective and versatile method to evaluate the skin oxidative state and the pro-oxidate and antioxidative effects of topical skin treatments on the iAOC of the skin. Therefore, the model can be considered to be a valuable tool in skin research.

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“…For example Grazul‐Bilska et al. determined the effects of skin moisturizers on total antioxidant capacity of human skin using EpiDerm™ (MatTek Corporation, Ashland, MA, USA) model and glutathione peroxidase activity as a marker of antioxidant capacity.…”

Section: In Vitro Antioxidant Activity In Skin Cultured Cellsmentioning

confidence: 99%

Methods for evaluation of cosmetic antioxidant capacity

Ratz-Łyko

Arct

Pytkowska

2011

Skin Research and Technology

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The skin as the largest part of human body is one of the main targets for ultraviolet radiation, environmental pollution, toxic chemicals and some metal ions, which share responsibility for the formation of free radicals. The resulting free radicals, both oxygen and nitrogen species are one of the main causes of aging due to impaired regulation of cell respiratory metabolism involving incomplete oxygen reduction in mitochondria and production a superoxide anion, hydroxyl radicals et al. In modern cosmetology to minimize the adverse effects of free radicals, antioxidants, which inhibit free radical reactions, mainly autoxidation processes are used. Currently, not only many cosmetic products containing antioxidants are available, but a large diversity of methods for determination of cosmetics antioxidant activity is also accessible. These methods can be divided into three main groups: in vitro, in vivo, and ex vivo as reported herein. Due to lack of standardization and validation it is necessary to use a variety of methods as well as conditions for those purposes, which are presented to the context.

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Antioxidant capacity of 3D human skin EpiDerm^TM model: effects of skin moisturizers

Cited by 9 publications

References 56 publications

Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

Assessing the Oxidative State of the Skin by Combining Classical Tape Stripping with ORAC Assay

Methods for evaluation of cosmetic antioxidant capacity

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Antioxidant capacity of 3D human skin EpiDermTM model: effects of skin moisturizers

Cited by 9 publications

References 56 publications

Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

Assessing the Oxidative State of the Skin by Combining Classical Tape Stripping with ORAC Assay

Methods for evaluation of cosmetic antioxidant capacity

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Product

Resources

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Antioxidant capacity of 3D human skin EpiDerm^TM model: effects of skin moisturizers