Antioxidant effect of lycopene-enriched tomato paste on N-nitrosodiethylamine-induced oxidative stress in rats

Abstract

Lycopene is a carotenoid pigment produced by vegetables and fruits, with tomatoes and their processed products being the most abundant sources. A high number of conjugated dienes make lycopene a powerful radical scavenger. Its antioxidant properties are considered to be primarily involved in many beneficial health effects. The present study was designed to assess the protective effect of lycopene-enriched tomato paste against N-nitrosodiethylamine (NDEA)-induced oxidative stress in rats. Forty-eight male Wistar rats were divided randomly into six groups. Four groups were treated with tomato paste, per os, for 28 days in doses which were equivalent to 0.5 (groups II and V) and 2.5 mg/kg b.w./day of lycopene (groups III and VI). Rats from groups IV–VI were given intraperitoneally a single dose of NDEA, 150 mg/kg b.w. Group I (control) was given distilled water. Pretreatment with tomato paste protected the antioxidant enzymes: superoxide dismutase, catalase and glutathione reductase. Their activity was recovered by 32–97 %, as compared to NDEA-treated rats. Microsomal lipid peroxidation in the liver was decreased in rats pretreated with a lower dose of tomato paste by 28 %, as compared to animals given NDEA alone. Pretreatment with tomato paste caused a decrease in plasma concentration of protein carbonyls, even below the control level, in rats given NDEA. Moreover, a 10 % reduction of DNA damage in leucocytes caused by NDEA was observed. The tomato paste tested was able to suppress NDEA-induced oxidative stress in rats.

Evaluation of the Liver Cancer Prevention of Anthocyanin Extracts from Mulberry (<i>Morus alba</i> L.) Variety PR-01

“…1.88-fold, which is consistent with earlier reports [13] [27]. Compared with the NDEA group, the hepatic tissue TBARS were significantly reduced in the C3G-150, MAEs-75 and MAEs-150 groups by 39.01%, 40.85%, and 44.52%, respectively ( Table 6, P < 0.001).…”

“…Our results showed that NDEA administration changes the antioxidant status, leading to oxidative stress, Whereas MAEs can inhibit lipid peroxidation and enhance antioxidant capacity, so the prevention effect of MAEs against NDEA-induced hepatocarcinogenesis in rats may be closely related to its antioxidant activity. Chen et al [13] [18] [27] also found that anthocyanins and water extracts from mulberry fruit can scavenge free radicals and shown concentration-dependent antioxidant activity in vitro.…”

“…Mulberry (Morus alba L.) fruit has been traditionally used in Chinese medicines for its pharmacological effects including antioxidant activity, anti-inflammatory, hepatoprotective effect, and anti-diabetic properties, etc. [13]. Mulberry is rich in anthocyanins (The anthocyanins content was 11.20 mg•100g −1 -193.00 mg•100g −1 in mulberry varieties), among which, the C3G and cyaniding-3-rutinoside (C3R) have been demonstrated to dose-dependently inhibit the migration and invasion of A549 human lung carcinoma cells [14].…”

Unifying Mechanism for Nutrients as Anticancer Agents: Electron Transfer, Reactive Oxygen Species and Oxidative Stress

“…Administration of the polyene provided protection from mitochondrial dysfunction and OS (Snadhir, Mehrotra, & Kamboj, 2010). The compound is a powerful radical scavenger and its AO properties are involved in many health effects (Kujawska, Ewertowska, Adamska et al, 2014). A tomato paste suppressed OS in rats.…”

Hematoprotective Effect of N(G)-Nitro-L-Arginine Methyl Ester and Lycopene on Hematoxicity in Trinitrobenzene Sulfonic Acid-Induced Colitis in Rat

“…Some studies into lycopene determined that it decreases levels of malondialdehyde (MDA), a product known to correlate with oxidative damage thanks to its association with the oxidation of fatty acid, and that lycopene increases the activities of such antioxidants as superoxide dismutases (SOD) and glutathione peroxidase (GP) [14]. A studyclearly demonstrate that lycopene is able to suppress NDEA-induced oxidative stress in rats [19].Altough a lot of studies were focused on the antioxidant and anti- …”