Antioxidant effects of hydroalcoholic and polyphenolic extracts of Peucedanum pastinacifolium Boiss. & Hausskn.

Abstract

Antioxidant activity of Peucedanum pastinacifolium Boiss. & Hausskn aerial part hydroalcoholic extract (HAE) and polyphenolic extract (PPE) as well as their total phenolic and flavonoid contents were studied. Phenolic and flavonoid contents were respectively estimated as gallic acid and quercetin equivalents. The in vitro antioxidant activity of two extracts of P. pastinacifolium were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), chelating activity on ferrous ions, or ferric reducing antioxidant power (FRAP) assay. In addition, the in vivo antioxidant activity of hydroalcoholic extract was measured by FRAP assay. Total phenolic contents of PPE and HAE were 117.1 ± 6.2 and 44.3 ± 1.7 mg/g, respectively. Total flavonoid content of PPE (43.4 ± 2.1 mg/g) was found to be higher than that of HAE (8.0 ± 1.5 mg/g). In DPPH radical scavenging assay, HAE and PPE showed fifty percent inhibitory concentration (IC50) values of 469.4 ± 9.3 μg/mL and 128.2 ± 5.5 μg/mL, respectively. Iron chelating activity assays indicated IC50 values of 657.5 ± 13.2 μg/mL and 735.4 ± 16.1 μg/mL for HAE and PPE as opposed to ethylenediamine tetra-acetic acid (EDTA) being 16.5 ± 0.8 μg/mL. PPE exhibited greater FRAP value (154.0 ± 1.8 μM) as compared with that of HAE being 69.3 ± 1.4 μM. In animal study, HAE showed a significant (p < 0.05) increase in FRAP level when compared with that of control group. Our results showed that P. pastinacifolium possess antioxidant properties which most likely are exerted through free radical scavenging, chelating activity, and reducing power.

Aerial Parts of Peucedanum chenur Have Anti-Cancer Properties through the Induction of Apoptosis and Inhibition of Invasion in Human Colorectal Cancer Cells

“…Over the past 20 years, more than 50% of commonly used drugs have been derived directly from plant derivatives, or some of the plant derivatives were considered as patterns to prepare some effective drugs [ 2 ] . The effects of Peucedanum to chemopreventive activity [ 20 ] , anti-proliferative activity [ 21 , 22 ] , induction of apoptosis [ 23 ] , growth retardation [ 24 ] , antioxidant properties [ 14 ] , limitation of cellular invasion [ 25 ] , and synergistic effects with other anticancer drugs [ 22 ] .…”

“…Antioxidant substances can protect the cell from oxidative damage through neutralizing the activity of free radicals [ 43 ] . Antioxidant effect of several genus of Peucedanum has been confirmed [ 14 ] .…”

“…The methanolic extract of Peucedanum graveolens has a high thermal and pH stability, and its antioxidant effect increases at high temperatures and pH levels [ 44 ] . In this study, two antioxidant tests, DPPH and iron chelating, were used [ 14 ] . Results clearly indicated a direct association between antioxidant activity and P. chenur methanolic extract concentration.…”

“…Tepe et al [ 45 ] suggested that essential oils obtained from Peucedanum longifolium and Peucedanum palimbioides extracts have 8.59% and 10.67% free radical scavenging activity of DPPH at 0.2 mg of concentration and have 12.42% and 90.39% Iron ions chelating activity at 2 mg of concentration, respectively. Meanwhile, DPPH free radicals scavenging IC 50 for polyphenols and flavonoids derived from Peucedanum Pastinacifolium hydroalcoholic extract has been indicated to be equal to 469.4 μg, and this value for iron chelating was equal to 657.5 μg [ 14 ] . As a result, P. chenur methanolic extract has higher antioxidant ability compared to other Peucedanum species at lower concentration; thus, P. chenur methanolic extract may be introduced as a new antioxidant agent in the field of medicine in the future.…”

“…Antioxidant activity of the extract was evaluated by DPPH and iron ions chelating assays [ 14 ] . Antioxidant activity of different concentrations of P. chenur extract was calculated as a percentage relative to the positive control.…”