Antioxidant enriched fraction fromPueraria tuberosaalleviates ovariectomized-induced osteoporosis in rats, and inhibits growth of breast and ovarian cancer cell linesin vitro

Abstract

Pueraria tuberosa (P. tuberosa), known as Indian Kudzu belongs to family Fabaceae and it is solicited as “Rasayana” drugs in Ayurveda. In the present study, we analyzed the efficacy an antioxidant enriched fraction (AEF) from the tuber extract of P. tuberosa against menopausal osteoporosis and breast and ovarian cancer cell lines. The AEF from P. tuberosa was identified by determining phenolic composition (total phenolic and flavonoid amount). Antioxidant property (in vitro assays) was also carried out followed by analysis of the AEF for its antiosteoporotic and anticancer potentials. Antiosteoporotic activity of AEF was investigated in ovariectomy-induced osteoporosis in rats and in vitro anticancer activity by MTT assay. Also, the GC/MS analysis of AEF was performed to determine various phytoconstituents. A docking analysis was performed to verify the interaction of bioactive molecules with estrogen receptors (ERs). Ethyl acetate fraction of the mother extract was proved as the AEF. AEF significantly improved various biomechanical and biochemical parameters in a dose dependent manner in the ovariectomized animals. AEF also controlled the increased body weight and decreased uterus weight following ovariectomy. Histopathology of femur revealed the restoration of typical bone structure and trabecular width in ovariectomized animals after AEF and raloxifene treatment. AEF also exhibited in vitro cytotoxicity in breast (MCF-7 and MDA-MB-231) and ovarian (SKOV-3) cancer cells. Further, genistein and daidzein exhibited a high affinity towards both estrogen receptors (α and β) in docking study revealing the probable mechanism of the antiosteoporotic activity. GC/MS analysis confirmed the presence of bioactive molecules such as stigmasterol, β-sitosterol, and stigmasta-3,5-dien-7-one. The observations of this study vindicate the potency of AEF from P. tuberosa in the treatment of menopausal osteoporosis and cancer.

Pueraria tuberosa: A Review on Traditional Uses, Pharmacology, and Phytochemistry

“…However, the mechanism behind this activity needs exploration for therapeutic use ( Satpathy et al, 2018 ). Antioxidant-enriched fraction also exhibited in vitro cytotoxicity in the breast (MCF-7 and MDA-MB-231) and ovarian (SKOV-3) cancer cells ( Satpathy et al, 2020 ).…”

“…Studies also showed a considerable decrease in the quantity of mature Leydig cells, cauda epididymis, and sperm motility ( Gupta et al, 2005 ). The antioxidant-enriched fraction from the tuber extract of P. tuberosa against menopausal osteoporosis in ovariectomy-induced osteoporosis in rats was studied and found that it improved biochemical parameters, controlled the increased body weight, and decreased uterus weight following ovariectomy as well as restoration of typical bone structure and trabecular width of the femur ( Satpathy et al, 2020 ). Incision and excision wounds were treated with methanolic and ethyl acetate tuber extract of P. tuberosa .…”

“…Phytochemical analysis of P. tuberosa extract using HPTLC revealed the presence of carbohydrates, proteins, alkaloids, flavonoids, saponins, phenols, and tannins ( Viji and Paulsamy, 2018 ). Satpathy et al showed the presence of 23 bioactive molecules including stigmasterol [25], β-sitosterol, and stigmasta-3,5-dien-7-one by gas chromatography–mass spectrometry analysis of antioxidant-enriched fraction prepared from P. tuberosa ( Satpathy et al, 2020 ). We have listed various phytoconstituents isolated from P. tuberosa and provided detailed information about their chemical structures, IUPAC names, and pharmacological activities, as well as associated references, in Table 2 .…”

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

“…In vitro cytotoxicity and anticancer activities [28] Formononetin human osteosarcoma cell lines (U2OS)/0-80 µM…”

“…Recently, various studies have been conducted to survey the cytotoxic activity of plant-derived compounds, such as isoflavones, against human cancer cells [ 28 , 29 ]. Despite the extensive investigation of their promising biological potentials, the study of possible cytotoxic potency of isoflavones remains important.…”