Antioxidant Potential, DNA Protection, and HPLC-DAD Analysis of Neglected MedicinalJurinea dolomiaeaRoots

Abstract

Jurinea dolomiaea Boiss., family Compositae, is a medicinally important plant of alpine region. Its tuberous roots are used in various ailments in folk medicine. This study was undertaken to estimate total phenolic (TPC) and total flavonoid contents (TFC) and to determine anti-free radical potential by diverse in vitro antioxidant assays. Crude methanol extract (JDME) was fractionated into n-hexane (JDHE), chloroform (JDCE), ethyl acetate (JDEE), n-butanol (JDBE), and aqueous (JDAE) fractions. The results indicated that JDEE and JDCE constituted the highest amount of TFC (807 ± 7.2 mg rutin equivalent/g sample) and TPC (757 ± 9.4 mg gallic acid equivalent/g sample), respectively. Significant correlation of TFC with IC50 values was recorded for •OH (R 2 = 0.91), H2O2 (R 2 = 0.82), and ABTS (R 2 = 0.82) assay. It could be made clear that JDEE was the most potent in antioxidant activity as compared to others, with generally lower IC50 values for DPPH (41.1 ± 1.0 μg/mL), ABTS (46.7 ± 0.6 μg/mL), H2O2 (42.2 ± 0.9 μg/mL), •OH (61.1 ± 1.1 μg/mL), O2 − (152 ± 1.1 μg/mL), and antilipid peroxidation (54.3 ± 1.6 μg/mL). HPLC chromatogram of JDEE revealed the presence of catechin, caffeic acid, and rutin. The results indicated the antioxidant activities of J. dolomiaea roots and merit further investigations for their use in oxidative stress related disorders.

Hepatic Regeneration and Reno-Protection by Fish oil, <i>Nigella sativa</i> Oil and Combined Fish Oil/<i>Nigella sativa</i> Volatiles in CCl₄ Treated Rats

“…All these changes are expected consequences of treatment with CCl 4 which induces elevation of reactive oxygen species ROS and MDA that lead to destruction and disruption of lipids of the membranous system, cellular proteins, intracellular organelles and causes DNA damage inducing several pathological changes 34,37 . CCl 4 is transformed in the liver to trichloromethyl free radical that disrupts Ca 2 homeostasis and induces lipid peroxidation that leads to hepatocellular injury 38 .…”

The Plant Natural Products: Their Antioxidants, Free Radical Scavengers, DNA Protection and Antimicrobial Activities

“…The normal levels of ROS play important roles in physiological mechanisms such as cellular signaling pathways and responses to infectious agents at low and moderate concentrations [14]. On the contrast, the over production of ROS in human body can cause oxidative damage for biomolecules as RNA and DNA, resulting in cell cellular damage and death [17].…”

“…Although, the artificial antioxidants such as butylated hydroxytoluene (BHT) or butylated hydroxyanisole (BHA) are commonly used in food industry as neutralizing agents of free radicals, their uses is linked with toxicity or mutations problems [14]. Hence, it's very important to find normal alternative antioxidants with high safety.…”

“…In addition, plants are also rich with flavonoids which are considered excellent source of antioxidants due to their advantages as hydrogen donor, reducing agent, and singlet oxygen scavenger. Furthermore, flavonoids are able to sequester the oxidative damage of unsaturated fatty acids and are used as an ideal preservative alternative to synthetic antioxidants in food industry [14].…”

МОНГОЛ ЧОНОГОНО (JURINEA MONGOLICA Maxim.) УРГАМЛЫН ФИТОХИМИ БОЛОН БИОЛОГИЙН ИДЭВХИЙН СУДАЛГАА

“…ургамлын газрын дээд хэсгийн метанолын хандны антиоксидант идэвхийг Пакистан улсын Jurinea dolomiaea Boiss. ургамлын үндэснээс гарган авсан төрөл бүрийн ханданд хийсэн үр дүнтэй жишиж үзэхэд харьцангуй өндөр идэвх үзүүлэв [24].…”