Antioxidant properties of amniotic membrane: novel observations from a pilot study

Lockington, David; Agarwal, Pankaj; Young, David; Ramaesh, Kanna

doi:10.1016/j.jcjo.2014.07.005

Cited by 14 publications

(14 citation statements)

References 47 publications

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“…34 High-molecular-weight HA contributes to the anti-inflammatory and anti-scarring properties of placental membranes, and recently, it has been reported that HA also serves as a free radical scavenger. 46,47 …”

Section: Extracellular Matrix Of Amnion and Chorionmentioning

confidence: 99%

Use of Placental Membranes for the Treatment of Chronic Diabetic Foot Ulcers

Brantley

Verla

2015

Advances in Wound Care

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Significance: Chronic diabetic foot ulcers (DFUs) remain a challenge for physicians to treat. High mortality rates for DFU patients have pointed to the low effectiveness of standard care and lack of quality wound care products. The composition (collagen-rich tissue matrix and endogenous growth factors and cells) and functional properties (anti-inflammatory, anti-bacterial, and angiogenic) of placental membranes are uniquely suited to address the needs of chronic wounds. This led to the commercialization of placental membranes, which are now widely available to physicians as a new advanced wound treatment option.Recent Advances: Progress in tissue processing and preservation methods has facilitated the development of placental products for wounds. Currently, a variety of commercial placental products are available to physicians for the treatment of chronic DFUs and other wounds. This review summarizes the key factors that negatively impact DFU healing (including social factors, such as smoking, vascular deficiencies, hyperglycemia, and other metabolic abnormalities), describes the structure and biology of placental membranes, and overviews commercially available placental products for wounds and data from the most recent DFU clinical trials utilizing commercial placental membranes.Critical Issues: Although the effects of diabetes on wound healing are complex and not fully understood, some of the key factors and pathways that interfere with healing have been identified. However, a multidisciplinary approach for the assessment of patients with chronic DFUs and guidelines for selection of appropriate treatment modalities remain to be implemented.Future Directions: The biological properties of placental membranes show benefits for the treatment of chronic DFUs, but scientific and clinical data for commercially available placental products are limited. Therefore, we need (1) more randomized, controlled clinical trials for commercial placental products; (2) studies that help to understand the timing of placental products' application and criteria for patient selection; and (3) studies comparing the functional properties of different commercially available placental products.

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Section: Extracellular Matrix Of Amnion and Chorionmentioning

confidence: 99%

Use of Placental Membranes for the Treatment of Chronic Diabetic Foot Ulcers

Brantley

Verla

2015

Advances in Wound Care

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“… 3 However, prolonged exposure to ROS and decreased levels of antioxidants associated with chronic wounds result in increased oxidative stress; this has been demonstrated in animal studies. 12 , 13 In a diabetic mouse model reported by Dhall et al , a high level of oxidative stress resulted in the formation of chronic wounds that were then reversed by treatment with antioxidants. 12 It has been recommended that exogenous antioxidants should be included in the treatment regimen for chronic wounds to control ROS imbalance.…”

Section: Discussionmentioning

confidence: 99%

“…One recent study by Lockington et al examined the antioxidant potential of hAM and found that it can inactivate hydrogen peroxide, a common oxidant. 13 In our study, we utilized a tbOOH-induced HDF oxidative injury model to evaluate the antioxidant potential of int-hAM and to compare it to dev-hAM. The potential of both the hAM tissue samples and of hAM-derived CM was studied to elucidate the contribution of the tissue matrix and to determine if released soluble antioxidant factors were present.…”

Section: Discussionmentioning

confidence: 99%

Soluble Factors Released by Endogenous Viable Cells Enhance the Antioxidant and Chemoattractive Activities of Cryopreserved Amniotic Membrane

Duan-Arnold

Gyurdieva

Johnson

et al. 2015

Advances in Wound Care

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Objective: Regulation of oxidative stress and recruitment of key cell types are activities of human amniotic membrane (hAM) that contribute to its benefits for wound treatment. Progress in tissue preservation has led to commercialization of hAM. The majority of hAM products are devitalized with various degrees of matrix alteration. Data show the importance of hAM matrix preservation, but little is known about the advantages of retaining viable endogenous cells. In this study, we compared the antioxidant and chemoattractive properties of viable intact cryopreserved hAM (int-hAM) and devitalized cryopreserved hAM (dev-hAM) to determine the benefits of cell preservation.Approach: We evaluated the ability of int-hAM and dev-hAM to protect fibroblasts from oxidant-induced cell damage, to suppress oxidants, and to recruit fibroblasts and keratinocytes in vitro.Results: Both the int-hAM–derived conditioned medium (CM) and the int-hAM tissue rescued significantly more fibroblasts from oxidant-induced damage than dev-hAM (844% and 93% more, respectively). The int-hAM CM showed a 202% greater antioxidant capacity than dev-hAM. The int-hAM CM enhanced the recruitment of fibroblasts and normal and diseased keratinocytes to a greater extent than dev-hAM (1,555%, 315%, and 151% greater, respectively).Innovation and Conclusion: Int-hAM, in which all native components are preserved, including endogenous viable cells, demonstrated a significantly greater antioxidant and fibroblast and keratinocyte chemoattractive potential compared to dev-hAM, in which viable cells are destroyed. The release of soluble factors that protect fibroblasts from oxidative injury by hAM containing viable cells is a mechanism of hAM antioxidant activity, which is a novel finding of this study.

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“…This property is specifically attributed to abundantly present hyaluronic acid in the amniotic mebrane. 2 On the other hand, there are distinct clinical entities that oxidative stress is particularly involved in the pathogenesis. For instance, the role of oxidative stress in chemical injuries, such as mustard agents and chronic venous ulcers, is well defined.…”

Section: Amniotic Membrane: New Concepts For An Old Dressingmentioning

confidence: 99%