Antioxidant status and dietary lipid unsaturation modulate oxidative DNA damage

Haegele, Albert D.; Briggs, Stephanie; Thompson, Henry J.

doi:10.1016/0891-5849(94)90247-x

Cited by 61 publications

(24 citation statements)

References 22 publications

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“…DNA isolated from peripheral blood leukocytes of breast cancer patients yields evidence of oxidative damage [17]. We have detected 8-hydroxy-2'-deoxyguanosine, an indicator of oxidative DNA damage, in murine mammary tumors (unpublished data) confirming published reports of oxidative DNA damage in both human 1-18] and rodent [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] breast tumors.…”

Section: Discussionsupporting

confidence: 68%

Sublethal oxidative stress inhibits tumor cell adhesion and enhances experimental metastasis of murine mammary carcinoma

1995

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We have postulated that murine mammary tumor progression is fueled, in part, by tumor-associated macrophages that deliver sub-lethal oxidative stress to tumor cells. In the present study, we determined whether oxidative stress would affect murine mammary tumor cell attachment to laminin and fibronectin, critical functions in the metastatic process. Sublethal oxidative stress generated by exposure of cells to hydrogen peroxide (H2O2, 1-1000 microM/L) inhibited tumor cell attachment to immobilized laminin or fibronectin. This oxidant effect was blocked in the presence of catalase which removes H2O2. The inhibitory effect on attachment was rapid, with significant inhibition occurring at 5 min; total inhibition was achieved at 60 min with 1 mM H2O2. The oxidative stress effect was partially reversible at 20 h post-treatment and occurred at concentrations of H2O2 that do not adversely affect cell viability or growth. Pretreatment of tumor cells with H2O2 or hypoxanthanine and xanthine oxidase (to generate superoxide radical and H2O2) prior to intravenous injection, enhanced experimental lung tumor colony formation. The enhancement of experimental metastatic potential with enzyme-generated oxidative stress was completely reversed by catalase; the H2O2-mediated enhancement was only partially reversed with catalase. Thus, treatments that inhibit tumor cell attachment to extracellular matrix proteins in vitro enhance experimental metastasis in vivo.

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Section: Discussionsupporting

confidence: 68%

Sublethal oxidative stress inhibits tumor cell adhesion and enhances experimental metastasis of murine mammary carcinoma

1995

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“…In animal models, polyunsaturated fats (PUFA) that contain high levels of linoleic acid have been shown to have strong promoting effects on mammary gland tumors, unlike other dietary fats [8, 9]. This may be related to the ease with which PUFA are peroxidized [10]. Mediterranean diets also have been shown to improve insulin resistance and decrease estrogen levels [11, 12].…”

Section: Introductionmentioning

confidence: 99%

A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters

Djurić¹,

Ren²,

Blythe³

et al. 2009

Nutrition Research

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This study examined patterns of changes in plasma fatty acids and carotenoids when women were asked to follow a novel, Greek-Mediterranean exchange list diet. A total of 69 healthy, non-obese, women ages 25–59, were randomized to either continue their own usual diet or to follow a modified Mediterranean diet for six months. There were no significant changes in blood lipids, triacylglycerol, insulin, glucose or C-reactive protein. Mean plasma carotenoids increased by 55%, which is consistent with a large increase in fruit and vegetable consumption. Likewise, changes in fat intakes were reflected in blood fatty acids, with a 25% increase in mean plasma monounsaturated fatty acids (MUFA). Principal component analysis was conducted to examine the sources of inter-individual variation for changes in carotenoid and fatty acid levels. Changes in the Mediterranean diet were clustered together in four components that accounted for 78% of the variance in plasma levels. Increases in plasma lutein, α- and β-carotene clustered together in a “vegetable” pattern, and increases in carotenoids found in fruit, β-cryptoxanthin and zeaxanthin, also clustered together but accounted for less of the variance. Increases in plasma MUFA were clustered with a decrease in plasma polyunsaturated fatty acids, consistent with substitution in the type of oils consumed. The only association of fatty acid levels with carotenoids was that of lycopene, which clustered together with an increase in saturated fatty acids. The changes in blood levels indicate the exchange list diet was effective for targeting Mediterranean nutrient intakes using foods available in the United States.

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“…Oxidative DNA damage has been shown to be related to the peroxidation of membrane fatty acids and low antioxidant status (Haegele et al, 1994), both present in diabetes. In the present study, the levels of TBARS and biomarkers of oxidative stress were unchanged by the administration of WP and 6-MSITC.…”

Section: Discussionmentioning

confidence: 99%

6-Methylsulfinylhexyl Isothiocyanate, an Antioxidant Derived from Wasabia japonica MATUM, Ameliorates Diabetic Nephropathy in Type 2 Diabetic Mice

Fukuchi¹,

Kato²,

Okunishi³

et al. 2004

FSTR

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Many studies have examined the protective effects of antioxidative agents against diabetic nephropathy, using various models. 6-Methylsulfinylhexyl isothiocyanate (6-MSITC) isolated from wasabi (Wasabiajaponica MATUM) induces glutathione S-transferase in vitro, thus 6-MSITC may act as an antioxidant in vivo. The aim of this study was to examine whether wasabi powder (WP) and 6-MSITC suppress oxidative stress in vivo and inhibit the impairment of renal function and diabetic nephropathy, using type 2 diabetic mice. KK-Ay type 2 diabetic mice were assigned to three groups (n = 10 each); control mice were fed normal chow (CRF-1) and two experimental groups were fed CRF-1 containing 0.5% WP or 0.03% 6-MSITC for 4 wk. Urine volume, urinary albumin excretion, and creatinine clearance were significantly lower in the 6-MSITC group than in the control group. There was statistically no difference in TBARS or other biomarkers of oxidative stress among the three groups. However, urinary 8-hydroxy-2'-deoxyguanosine (8-0HdG), one of the markers of oxidative stress tended to be lower in the 6-MSITC group than in the control group. In conclusion, the present results show that a sufficient supply of dietary 6-MSITC may prevent or delay renal dysfunction in diabetes by protecting against oxidative stress, and that dietary 6-MSITC may have beneficial effects on diabetic complications in type 2 diabetic mice.

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Antioxidant status and dietary lipid unsaturation modulate oxidative DNA damage

Cited by 61 publications

References 22 publications

Sublethal oxidative stress inhibits tumor cell adhesion and enhances experimental metastasis of murine mammary carcinoma

Sublethal oxidative stress inhibits tumor cell adhesion and enhances experimental metastasis of murine mammary carcinoma

A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters

6-Methylsulfinylhexyl Isothiocyanate, an Antioxidant Derived from Wasabia japonica MATUM, Ameliorates Diabetic Nephropathy in Type 2 Diabetic Mice

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