Antioxidant status of the potato tuber and Ca²⁺ deficiency as a physiological stress

Abstract

The antioxidant status of potato (Solanum tuberosum L.) tubers of two genotypes, cv. Désirée and clone 10337de40 was investigated in relation to susceptibility to internal rust spot (IRS), a Ca2+‐related physiological disorder. Concentrations of total calcium within the perimedulla tissue of tubers, grown with a restricted (1 mM CaCl2) Ca2+ supply, were similar in cv. Désirée (IRS resistant) and clone 10337de40 (IRS susceptible). A range of antioxidants was assayed in order to assess antioxidant status in both genotypes under the two Ca2+ treatments. Although no appreciable differences were detected between low Ca2+ and control treatments, certain antioxidants were present at significantly higher levels in the IRS resistant genotype, cv. Désirée. These included dehydroascorbate reductase (EC 1.8.5.1) activity (more than 100% higher), total glutathione content (ca 40% higher), glutathione reductase (EC 1.6.4.2) activity (almost 50% higher), peroxidase (EC 1.11.1.7) activity (ca 60% higher) and superoxide dismutase (EC 1.15.1.1) activity (almost 80% higher). There was no difference in ascorbate content, ascorbate free radical reductase activity (EC 1.6.5.4), α‐tocopherol levels and catalase activity (EC 1.11.1.6) between the two genotypes. The possible relationship between resistance to IRS and a superior antioxidant status, found in cv. Désirée, is discussed.

Salt Tolerance of Cotton: Some New Advances

“…It is now known that activated oxygen species are generally produced in high amounts in plants subjected to different environmental stresses such as high and low temperatures, water deficit, high light intensity, herbicide treatment, or mineral nutrient deficiency, and these activated species cause a severe oxidative damage (Dhindsa and Matowe, 1981;Wise and Naylor, 1987;Monk and Davies, 1989;Cakmak and Marschner, 1990;. It has been widely reported that plants possessing high concentrations of antioxidants have high resistance to the oxidative damage caused by the activated oxygen species (Wise and Naylor, 1987;Spychalla and Desborough, 1990).…”

Antioxidant Response to NaCl Stress in a Control and an NaCl-Tolerant Cotton Cell Line Grown in the Presence of Paraquat, Buthionine Sulfoximine, and Exogenous Glutathione

“…When plants are subjected to environmental stress, the balance between the production of reactive O, species and the quenching activity of antioxidants is upset, often resulting in oxidative damage (Harper and Harvey, 1978;Dhindsa and Matowe, 1981;Rabinowitch and Fridovich, 1983;Wise and Naylor, 1987;Monk and Davies, 1989;Spychalla and Desborough, 1990;Cakmak and Marschner, 1992;Polle et al, 1992;Asada, 1994;Krause, 1994). Plants with high levels of antioxidants, either constitutive or induced, have been reported to have a greater resistance to this oxidative damage (Harper and Harvey, 1978;Dhindsa and Matowe, 1981;Wise and Naylor, 1987;Monk and Davies, 1989;Spychalla and Desborough, 1990;Mandamanchi and Alscher, 1991;Polle and Rennenberg, 1994).…”

“…Plants with high levels of antioxidants, either constitutive or induced, have been reported to have a greater resistance to this oxidative damage (Harper and Harvey, 1978;Dhindsa and Matowe, 1981;Wise and Naylor, 1987;Monk and Davies, 1989;Spychalla and Desborough, 1990;Mandamanchi and Alscher, 1991;Polle and Rennenberg, 1994).…”

“…Vol. 112, 19961989Sen Gupta et al, 1991;May and Leaver, 1993;Creissen et al, 1994), both cell lines were also grown in the presence of BSO, a potent inhibitor of GSH synthesis (Griffith and Meister, 1979), and exogenous GSH and GSSG to help further elucidate the role of GR in the development of NaCl stress in cotton.…”

Superoxide Dismutase, Catalase, and α-Tocopherol Content of Stored Potato Tubers

“…This can upset the balance of oxygen free radical production and quenching, and tests the antioxidant capacity of the aerobic cell (3,4,7,13,21,24,26,30). High constitutive levels or high induced levels of antioxidants in a plant cell may provide resistance to a particular stress (6,15,30).…”