Antioxidative enzymes and substances involve in the activity of improving the oxidative tolerance of Pichia caribbica by ascorbic acid

Yang, Qiya; Li, Yulin; Li, Chaolan; Zhang, Hongyin; Jiang, Zhenhui; Zhang, Xiaoyun; Serwah, Boateng Nana Adwoa; Mahunu, Gustav Komla; Zhu, Shuyun

doi:10.1016/j.biocontrol.2017.02.013

Cited by 10 publications

(5 citation statements)

References 38 publications

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“…Rhodotorula mucilaginosa , 4 Saccharomyces cerevisiae , 5 Hanseniaspora uvarum 6 Pichia caribbica , 7 Cryptococcus podzolicus , 8 Sporidiobolus pararoseus Y16, 9 and Yarrowia lipolytica 10 are some of the yeasts reported having potential biocontrol efficiency. Though yeasts gained much recognition as biocontrol agents, their biocontrol efficiency is comparatively less than the commercially available synthetic fungicides 11 . Also, antagonistic yeasts are prone to oxidative stress, a primary defense mechanism of plants against invading pathogens 7 .…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, recent research has focused on increasing the biocontrol efficiency and oxidative stress tolerance of the yeasts. Chitinase, trehalose, ascorbic acid, chitosan, salicylic acid, phosphatidylcholine, and several other natural compounds have been used along with the antagonistic yeast to enhance the biocontrol efficiency and oxidative stress tolerance 6, 11–13 . Trehalose is a glucose derivative and acts as major carbohydrate reserve in microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Dhanasekaran

Yang

Godana

et al. 2021

Pest Management Science

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BACKGROUND In the process of biological control, the antagonistic yeasts contend with various stresses that negatively influence yeasts' biocontrol efficiency. In the current study, we investigated the effect of trehalose supplementation on the biocontrol efficiency and oxidative stress tolerance of Sporidiobolus pararoseus Y16. RESULTS S. pararoseus Y16, an antagonistic yeast cultured in trehalose supplemented medium, exhibited better biocontrol efficiency against Penicillium expansum and Aspergillus tubingensis in table grapes. Trehalose‐treated S. pararoseus Y16 cells showed good proliferation efficiency and oxidative stress tolerance than untreated cells. Increased β‐1,3‐glucanase, catalase, superoxide dismutase activity, and low protein carbonylation were observed in trehalose‐amended S. pararoseus Y16 upon H2O2 exposure. The RNA sequencing results indicated that trehalose significantly altered the transcriptome of S. pararoseus Y16. The GO, KEGG, and COG annotations revealed that the differentially regulated genes corresponded to the various biological process of the yeast. CONCLUSION Our findings suggested that trehalose use could enhance the biocontrol efficiency and oxidative stress tolerance of S. pararoseus Y16. Trehalose supplementation altered the transcriptome of S. pararoseus Y16, particularly the genes that correspond to amino acid metabolism, nucleotide metabolism, and protein modification. Thereby the oxidative stress tolerance and biological control efficiency of S. pararoseus Y16 was enhanced by trehalose. © 2021 Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Dhanasekaran

Yang

Godana

et al. 2021

Pest Management Science

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“…When plants are subjected to abiotic stress, large amounts of reactive oxygen species produced. The plant antioxidant can eliminate the damage caused by excessive ROS accumulation by regulating CAT, SOD, POD, and other antioxidant enzyme activities (Yang et al, 2017). The accumulation of osmoregulatory substances is an important indicator of plant stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the potato VQ31 enhances salt tolerance in Arabidopsis

Zhai,

Ao,

et al. 2024

Front. Plant Sci.

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Plant-specific VQ proteins have crucial functions in the regulation of plant growth and development, as well as in plant abiotic stress responses. Their roles have been well established in the model plant Arabidopsis thaliana; however, the functions of the potato VQ proteins have not been adequately investigated. The VQ protein core region contains a short FxxhVQxhTG amino acid motif sequence. In this study, the VQ31 protein from potato was cloned and functionally characterized. The complete open reading frame (ORF) size of StVQ31 is 672 bp, encoding 223 amino acids. Subcellular localization analysis revealed that StVQ31 is located in the nucleus. Transgenic Arabidopsis plants overexpressing StVQ31 exhibited enhanced salt tolerance compared to wild-type (WT) plants, as evidenced by increased root length, germination rate, and chlorophyll content under salinity stress. The increased tolerance of transgenic plants was associated with increased osmotic potential (proline and soluble sugars), decreased MDA accumulation, decreased total protein content, and improved membrane integrity. These results implied that StVQ31 overexpression enhanced the osmotic potential of the plants to maintain normal cell growth. Compared to the WT, the transgenic plants exhibited a notable increase in antioxidant enzyme activities, reducing cell membrane damage. Furthermore, the real-time fluorescence quantitative PCR analysis demonstrated that StVQ31 regulated the expression of genes associated with the response to salt stress, including ERD, LEA4-5, At2g38905, and AtNCED3. These findings suggest that StVQ31 significantly impacts osmotic and antioxidant cellular homeostasis, thereby enhancing salt tolerance.

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“…In order to reduce excessive ROS accumulation, yeast has evolved an antioxidant defense system that contains various enzymes and nonenzymatic components. Antioxidant enzymes that can directly react with and eliminate ROS include SOD, CAT, and POD ( 28 ). The decomposition of ROS depends on the antioxidant enzymes, such as CAT, and is closely related to the enhancement of the biological control ability of antagonistic yeast ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

“…Succinate dehydrogenase is an important enzyme in the mitochondrial respiratory system that produces ATP to provide sufficient energy for yeast cell activity by dehydrogenating succinate to fumarate and promoting yeast cell metabolism and nutrient utilization ( 37 ). Yang et al ( 28 ) showed that vitamin C increased the metabolic activity of yeast under oxidative conditions. Similarly in the present study, the vitamin C treatment also increased the metabolic activity of cells to various degrees, but the effect was not as strong as that of Ca ascorbate.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Biocontrol Efficacy of Pichia kudriavzevii Induced by Ca Ascorbate against Botrytis cinerea in Cherry Tomato Fruit and the Possible Mechanisms of Action

et al. 2021

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Antioxidative enzymes and substances involve in the activity of improving the oxidative tolerance of Pichia caribbica by ascorbic acid

Cited by 10 publications

References 38 publications

Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Overexpression of the potato VQ31 enhances salt tolerance in Arabidopsis

Enhancement of Biocontrol Efficacy of Pichia kudriavzevii Induced by Ca Ascorbate against Botrytis cinerea in Cherry Tomato Fruit and the Possible Mechanisms of Action

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