Antioxidative Role of Hygrophila erecta (Brum. F.) Hochr. on UV-Induced Photoaging of Dermal Fibroblasts and Melanoma Cells

Abstract

Antioxidants are an important strategy for treating photoaging because excessive reactive oxygen species (ROS) are produced during UV irradiation. The therapeutic effects of methanol extracts of Hygrophila erecta (Brum. F.) Hochr. (MEH) against UV-induced photoaging were examined by monitoring the changes in the antioxidant defense system, apoptosis, extracellular matrix (ECM) modulation, inflammatory response, and melanin synthesis in normal human dermal fibroblast (NHDF) cells and melanoma B16F1 cells. Four bioactive compounds, including 4-methoxycinnamic acid, 4-methoxybenzoic acid, methyl linoleate, and asterriquinone C-1, were detected in MEH, while the DPPH free radical scavenging activity was IC50 = 7.6769 µg/mL. UV-induced an increase in the intracellular ROS generation, NO concentration, SOD activity and expression, and Nrf2 expression were prevented with the MEH treatment. Significant decreases in the number of apoptotic cells, the ratio of Bax/Bcl-2, and cleaved Cas-3/Cas-3 were observed in MEH-treated NHDF cells. The MEH treatment induced the significant prevention of ECM disruption and suppressed the COX-2-induced iNOS mediated pathway, expression of inflammatory cytokines, and inflammasome activation. Finally, the expression of the melanin synthesis-involved genes and tyrosinase activity decreased significantly in the α-melanocyte-stimulating hormone (MSH)-stimulated B16F1 cells after the MEH treatment. MEH may have an antioxidative role against UV-induced photoaging by suppressing ROS-induced cellular damage.

Ginsenoside Rk1 Prevents UVB Irradiation-Mediated Oxidative Stress, Inflammatory Response, and Collagen Degradation via the PI3K/AKT/NF-κB Pathway In Vitro and In Vivo

“…A previous study verified that UVB exposure could destroy the equilibrium between oxidative and antioxidant defense systems in skin through generating excessive ROS, including H 2 O 2 , • OH, and O 2 •– , which could reduce the activities of antioxidant enzymes and induce an oxidative stress response. − Consistent with this finding, our research showed that topical administration of Rk1 significantly inhibited ROS overproduction during UV irradiation of BALB/c nude mouse skin and HaCaT keratinocytes. Additionally, antioxidant enzymes ( e.g.…”

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

“…Sunburn cells, representative keratinocytes undergoing apoptosis post UV exposure, are seen as a protective measure, eliminating potentially malignantly transformed cells [52]. Such a mechanism has been detailed in a plethora of studies, particularly emphasizing UV radiation's apoptotic impacts, evaluated primarily through the Annexin V-FITC/PI method via flow cytometry [59][60][61][62][63][64].…”

Kunzea ericoides (kanuka) leaf extracts show moisturisation, anti-oxidant, and UVB-protection effects in HaCaT cells and anti-melanogenesis effect in B16F10 cells

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