Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines

Azadi, Hamideh Ghodrati; Ghaffari, Seyed Mahmood; Riazi, Gholamhossein; Ahmadian, Shahin; Vahedi, Fatemeh

doi:10.1007/s10616-008-9145-0

Cited by 50 publications

(17 citation statements)

References 30 publications

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“…The MIC/MBCs of ASHE (32/128 μ g mL −1 ) and ASDE (64/128 μ g mL −1 ) for MRSA are comparatively lesser than the CC 50 concentrations on Vero cells (<383.4 μ g mL −1 ; <390.6 μ g mL −1 ) which is therapeutically acceptable for a nontoxic edible natural extract. Chloroformic extract of A. hirtifolium bulbs suppressed the growth of HeLa and MCR-7 cells at 200 μ g mL −1 [31], while the hydromethanolic extract of A. hirtifolium remains nontoxic to mammalian cells at 1500 μ g mL −1 [20]. These results are partly in agreement with our results, which for the first time report the in vivo antibacterial and burn wound healing activities of hexane and dichloromethane extracts prepared from the bulbs of A. stipitatum .…”

Section: Discussionsupporting

confidence: 90%

“…Significant amount of the presence of allicin (a major bioactive compound of Allium family) in the bulbs of A. hirtifolium Boiss. has also been demonstrated by TLC methods [31]. In contrast, only a low percentage of sulfur compounds including S-methyl methanethiosulfonate, 2,4,5-trithiahexane, 2,4-dithiapentane, 2-pyridinethione, and methane (chloromethylthio) (methylthio) were detected in the present study.…”

Section: Discussionsupporting

confidence: 44%

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Allium stipitatum Extract Exhibits In Vivo Antibacterial Activity against Methicillin‐Resistant Staphylococcus aureus and Accelerates Burn Wound Healing in a Full‐Thickness Murine Burn Model

Karunanidhi

Ghaznavi‐Rad

Nathan

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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The in vivo antibacterial and burn wound healing potency of Persian shallot bulbs (Allium stipitatum) were explored in a mice burn model infected with methicillin-resistant Staphylococcus aureus (MRSA). Hexane (ASHE) and dichloromethane (ASDE) extracts were tested. Female BALB/c mice were inflicted with third-degree thermal injury followed by infection with MRSA. ASHE and ASDE formulated with simple ointment base (SOB) at concentrations of 1%, 2%, and 5% (w/w) were topically applied to burn wounds twice a day for 20 days. Silver sulfadiazine (1%) served as drug positive control. Microbiological analysis was carried out on 1, 2, 3, 4, and 5 days postwounding (dpw) and histopathological analysis at the end of the experiment (20 dpw). Both ointments demonstrated strong antibacterial activity with complete elimination of MRSA at 48–72 h after infection. The rate of wound contraction was higher (95–100%) in mice groups treated with ASHE and ASDE ointments after 15 dpw. Histological analysis revealed significant increase (p < 0.05) in epithelialization and collagenation in treated groups. The ASHE and ASDE were found to be relatively noncytotoxic and safe to Vero cell line (383.4 μg mL−1; 390.6 μg mL−1), suggesting the extracts as safe topical antibacterial as well as promising alternatives in managing thermal injuries.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 44%

Allium stipitatum Extract Exhibits In Vivo Antibacterial Activity against Methicillin‐Resistant Staphylococcus aureus and Accelerates Burn Wound Healing in a Full‐Thickness Murine Burn Model

Karunanidhi

Ghaznavi‐Rad

Nathan

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…(Persian shallot) is one of the Iranian native spices [6, 13] which belong to the same biological genus of Allium sativum (garlic) and other onions [14]. It is one of the valuable members of Allium with its bulbs commonly used as a traditional remedy [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Antibacterial and Cytotoxic Activities ofAllium hirtifoliumBoiss

Ismail

Jalilian

Talebpour³

et al. 2013

BioMed Research International

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Allium hirtifolium Boiss. known as Persian shallot, is a spice used as a traditional medicine in Iran and, Mediterranean region. In this study, the chemical composition of the hydromethanolic extract of this plant was analyzed using GC/MS. The result showed that 9-hexadecenoic acid, 11,14-eicosadienoic acid, and n-hexadecanoic acid are the main constituents. The antibacterial activity of the shallot extract was also examined by disk diffusion and microdilution broth assays. It was demonstrated that Persian shallot hydromethanolic extract was effective against 10 different species of pathogenic bacteria including methicillin resistant Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA), Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Escherichia coli, Escherichia coli O157:H7, Salmonella typhimurium, Proteus mirabilis, and Klebsiella pneumoniae. Specifically, the minimum concentration of the extract which inhibited bacterial growth (MIC values) was 1.88 mg/mL for most of the gram-positive bacteria. This concentration was not much different from the concentration that was safe for mammalian cells (1.50 mg/mL) suggesting that the hydromethanolic extract of Persian shallot may be a safe and strong antibacterial agent.

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“…In Iranian folk medicine, mooseer has been successfully used for treating rheumatic and inflammatory disorders. In addition, different medicinal properties such as antitrichomonas, antiproliferative, and immunomodulatory activities have also been reported for the bulbs of mooseer (Mozaffarian, 2008;Ghodrati Azadi et al, 2008;Jafarian et al, 2003;Amin, 1991). Results of previous studies (Ashrafi et al, 2004;Ismail et al, 2013) indicated that the aqueous and methanol extracts of mooseer bulbs have antimicrobial properties.…”

Section: Introductionmentioning

confidence: 99%

Variation in antioxidant, and antibacterial activities and total phenolic content of the bulbs of mooseer (Allium hirtifolium Boiss.)

Pirbalouti¹,

Ahmadzadeh²,

Malekpoor³

2015

AAS

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Allium hirtifolium Boiss. (mooseer) belonging to the family Alliaceae, is an endemic species of Iran which grows wild in the Zagros Mountains range, western and southwestern Iran. The bulb of A. hirtifolium has been used as a flavouring agent, especially dairy foods and pickles by the indigenous people, southwestern Iran. In this study, the bulbs of various populations of the plant were collected from the alpine regions in Chaharmahal va Bakhtiari province, Iran. The total phenolic content of the ethanol extract was determined by FolinCiocalteu method, the antioxidant activity was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the antibacterial activity of the extracts against four bacteria, including Bacillus cereus, Listeria monocytogenes, Proteus vulgaris, and Salmonella typhimurium was determined by serial dilution assay. Results indicated that the total phenolic content in the ethanol extracts of different populations of A. hirtifolium ranged between 34 to 44 mg gallic acid/g extract. In addition, the extracts of A. hirtifolium indicated moderateto-good inhibitory activities (MICs = 0.062 to 0.250 mg/ml) against four bacteria, especially against B. cereus. The antioxidant activity of the bulbs of A. hirtifolium indicated the extract acted as an effective DPPH scavenger, but were not as effective as the BHT control. This finding suggests that the bulbs of A. hirtifolium may be considered as a natural source of antioxidants and antimicrobial agents.

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Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines

Cited by 50 publications

References 30 publications

Allium stipitatum Extract Exhibits In Vivo Antibacterial Activity against Methicillin‐Resistant Staphylococcus aureus and Accelerates Burn Wound Healing in a Full‐Thickness Murine Burn Model

Allium stipitatum Extract Exhibits In Vivo Antibacterial Activity against Methicillin‐Resistant Staphylococcus aureus and Accelerates Burn Wound Healing in a Full‐Thickness Murine Burn Model

Chemical Composition and Antibacterial and Cytotoxic Activities ofAllium hirtifoliumBoiss

Variation in antioxidant, and antibacterial activities and total phenolic content of the bulbs of mooseer (Allium hirtifolium Boiss.)

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