Antisense RNA mediated protection from white spot syndrome virus (WSSV) infection in Pacific white shrimp Litopenaeus vannamei

Akhila, Dharnappa Sannejal; Mani, Madhu K.; Rai, Praveen; Condon, Kelly; Owens, Leigh; Karunasagar, Indrani

doi:10.1016/j.aquaculture.2014.10.005

Cited by 14 publications

(9 citation statements)

References 40 publications

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“…Ponprateep et al, (2013) validated the interaction of wsv477 protein with SPIPm2, where SPIPm2 hindered the functional property of wsv477 which was also proved by RNAi studies. Shrimp treated with wsv477dsRNA offered higher protection compared to other targeted gene and this endorses with earlier work where antisense targeted on this gene provided 90% survival against WSSV infection (Akhila et al, 2015). Feed + VP24dsRNA + PBS + cod liver oil 10 X 3 RR1 Feed + RR1dsRNA + PBS + cod liver oil 10 X 3 wsv477…”

Section: Injectionsupporting

confidence: 78%

“…Non-structural gene, WSV477 encodes for 208 amino acid and these peptides have GTP-binding activity . Antisense construct on WSV477 offered potent and significant antiviral immunity against WSSV infection in comparison to other targeted envelope protein in Litopenaeus vannaemi (Akhila et al, 2015). The present study aimed at comparing the potentiality of dsRNA coding structural (VP26, VP24) and non-structural (RR1, WSV477) genes of WSSV and their possible effects against WSSV infection in Indian black tiger shrimp (Penaeus monodon).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477 Gene against WSSV Infection in Penaeus monodon

Puneeth¹,

Akhila²,

Dechamma³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Section: Injectionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477 Gene against WSSV Infection in Penaeus monodon

Puneeth¹,

Akhila²,

Dechamma³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Sintasan kumulatif udang windu Penaeus monodon yang diuji tantang dengan WSSV. Jurnal Riset Akuakultur, 13 (1), 2018, 57-65 dan 90% dibandingkan dengan kontrol (0%) pada hari ke-9 setelah ditantang dengan WSSV (Akhila et al, 2015). Peningkatan resistensi udang windu terhadap WSSV dengan teknologi transgenesis juga telah dilaporkan oleh Parenrengi (2010) dengan sintasan 24,5% lebih tinggi dibandingkan dengan kontrol.…”

Section: Aplikasi Vaksin Rekombinan Dsrna Vp-15unclassified

Pengklonan Gen Penyandi Viral Protein 15 (Vp-15) WSSV Dan Aplikasinya Sebagai Vaksin Rekombinan Pada Udang Windu

Parenrengi

Mulyaningrum

Tenriulo

et al. 2018

Jurnal Riset Akuakultur

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ABSTRAKInfeksi white spot syndrome virus (WSSV) dapat menyebabkan kematian massal pada budidaya udang windu Penaeus monodon di Indonesia. Infeksi yang terjadi secara sistematis tersebut disebabkan oleh peran gen nucleocapsid viral protein . Upaya pengembangan gen VP-15 WSSV untuk menginduksi respons imun dan menetralisasi terhadap infeksi WSSV pada udang windu perlu dilakukan. Penelitian ini bertujuan untuk mengisolasi dan merekombinasikan gen penyandi VP-15 WSSV sebagai vaksin dsRNA, serta menganalisis aplikasinya pada udang windu. Gen VP-15 diisolasi dari udang windu yang terinfeksi WSSV, dikloning ke dalam suatu vektor dan ditransformasikan ke sel kompeten (bakteri Escheria coli DH5). Plasmid diisolasi untuk mengonfirmasi insert region gen VP-15 melalui sekuensing nukleotida. Pembuatan vaksin rekombinan dilakukan secara in-vitro menggunakan kit MEGAscript RNAi dan diaplikasikan ke udang windu melalui metode injeksi dengan dosis tunggal 0,2 µg dan kontrol (tanpa injeksi vaksin). Hewan uji yang digunakan berukuran panjang 14,75±3,17 g dan bobot 11,64±0,76 cm; serta dipelihara pada wadah bak fiber volume 250 L dengan kepadatan 10 ekor/bak. Hasil penelitian menunjukkan bahwa gen penyandi VP-15 telah diisolasi dari udang windu dan vaksin rekombinan telah dihasilkan secara in-vitro. Analisis sekuens nukleotida memperlihatkan bahwa sisipan gen DNA VP-15 sebesar 253 bp dan menunjukkan kemiripan yang tinggi (99%) pada GenBank. Penggunaan vaksin rekombinan dsRNA dengan dosis 0,2 µg memperlihatkan sintasan udang windu yang dapat mencapai 40,0% dibandingkan dengan kontrol hanya 3,3% (peningkatan 36,7%). Gambaran histopatologi pada jaringan hepatopankreas udang windu pada perlakuan kontrol menunjukkan adanya kerusakan inti sel, akibat infeksi WSSV. Gene VP-15 berpotensi sebagai bahan vaksin rekombinan dsRNA dalam mencegah infeksi WSSV.

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“…japonicas (Namikoshi et al, 2004), and P. monodon (Witteveldt et al, 2004). The application of antisense RNA in protecting L. vannamei from WSSV infection was also reported by Akhila et al (2015). The dsRNA from VP-28 was reported to be useful in protecting Macrobrachium rosenbergii from WSSV (Jariyapong et al, 2015).…”

Section: Characteristic Of a Viral Protein (Vp-15) Of White Spot Syndmentioning

confidence: 87%

CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798)

Parenrengi

Alimuddin

Tenriulo

2017

Indonesian Aquaculture Journal

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White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.

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Antisense RNA mediated protection from white spot syndrome virus (WSSV) infection in Pacific white shrimp Litopenaeus vannamei

Cited by 14 publications

References 40 publications

Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477 Gene against WSSV Infection in Penaeus monodon

Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477 Gene against WSSV Infection in Penaeus monodon

Pengklonan Gen Penyandi Viral Protein 15 (Vp-15) WSSV Dan Aplikasinya Sebagai Vaksin Rekombinan Pada Udang Windu

CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798)

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