Antitumor activity of doxorubicine-loaded nanoemulsion against Ehrlich ascites carcinoma-bearing mice

Alkhatib, Mayson H.; Alkreathy, Huda M.; Balamash, Khadijah S.; Abdu, Faiza

doi:10.4314/tjpr.v15i5.6

Cited by 7 publications

(2 citation statements)

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“…The mice were split into eight groups (n=20) according to the administered drug, as shown in Table 1. The body weight of all mice was taken before injection with EAC cells maintained in the ascetic form as described by [9]. All of the mice in each group were injected intraperitoneally with 2.5×10 6 EAC cells/mouse on the 0th day.…”

Section: Inoculation Of Eac In the Micementioning

confidence: 99%

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In vivo Assessment of the Hepatotoxicity of Mixed Gemcitabine and 5-Fluorouracil Loaded on Microemulsion in Mice Bearing Ehrlich Ascites Carcinoma

Alkhatib¹,

Alasmari²,

Abdo³

2017

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A combination of two or more chemotherapeutic agents in a nanoparticle is a promising approach to improve the treatment effectiveness and reduce their toxicity. In this study, the formulations of 30mg/kg of gemcitabine (GEM) mixed with 2.5mg/kg of 5-fluorouracil (5-FU), either loaded on a microemulsion (GEM+5'FU-ME) or dissolved in water (GEM+5'FU-Sol), were examined to explore their antitumor activity in mice inoculated with Ehrlich ascites carcinoma (EAC). Eighty mice were split into eight groups (10 mice/group). Group I served as the untreated EAC bearing mice. Groups II-VIII were EAC bearing mice treated with free GEM (GEM-Sol), GEM-loaded-ME (GEM-ME), free 5-FU (5'FU-Sol), 5-FU-loaded-ME (5'FU-ME), GEM+5'FU-Sol, GEM+5'FU-ME and the blank-ME (B-ME), respectively. The hepatotoxicity was identified by determining the serum enzymes, and studying the histological changes of the liver tissues. The z-average diameter and zeta potential of the nanodroplets of GEM+5'FU-ME were 122.1±26.80nm and-4.40±1.76mV, respectively. Results demonstrated that drug-loaded ME formulations have lowered the enzyme levels of ALT, AST and ALP and did not cause damage in the hepatocytes when compared to the drug solution formulations. In conclusion, the combination treatments of GEM and 5-FU in a ME may protect the drugs from degradation in the liver.

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Section: Inoculation Of Eac In the Micementioning

confidence: 99%

“…Immediately following sacrificing the mice on the 17th day after the blood collection, the collected liver tissues were incubated in a fixation buffered formalin and were treated as described elsewhere [9].…”

Section: Histologymentioning

confidence: 99%