Antitumor vector systems based on bioactive lectin of Bacillus subtilis ІМВ B-7724

Petranovska, А. L.; Kusyak, А. P.; Корнійчук, Н. М.; Turanska, S. P.; Gorbyk, P. P.; Lukyanova, Natalia; Чехун, В Ф

doi:10.15407/hftp12.03.190

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…Одним із найважливіших результатів досліджень магніточутливих НК для протипухлинної терапії було виявлення загальної закономірності -синергізму сумісної дії хіміотерапевтичного препарату, антитіла та залізовмісного нанорозмірного носія, ефективність якої достовірно перевершувала дію відповідних лікарських засобів в індивідуальному використанні у тих же дозах [16,18,23,62,72,76,77]. Виявлений синергічний цитотоксичний/цитостатичний ефект дії залізовмісного нанобіокомпозиту на основі магнітної рідини і протипухлинного препарату та подолання in vitro, in vivo резистентності клітин і пухлин раку молочної залози людини MCF-7 було пояснено високою біологічною активністю комплексу Fe 3 O 4 з хіміотерапевтичним лікарським препаратом та фармакологічною корекцією процесів обміну ендогенного заліза з пухлиною [23,62].…”

Section: фізико-хімічні основи створення новітніх засобів для подолан...unclassified

“…Виходячи з наведених даних можна запропонувати новий фізико-хімічний принцип подолання лікарської резистентності злоякісних клітин та пухлин: діючу протипухлинну речовину використовувати в складі нанобіокомпозиту, в якому вона знаходиться в іммобілізованому стані на поверхні нанорозмірного магніточутливого залізовмісного носія. Реалізація цього принципу може бути досягнена, зокрема, шляхом хімічного конструювання магніточутливих нанокомпозитів типу ядро-оболонка з багаторівневою ієрархічною наноархітектурою, здатних виконувати комплекс функцій, характерних медико-біологічним нанороботам [9,[14][15][16][17][18][19][20][21][22][72][73][74][75][76][77][78][79][80].…”

Section: фізико-хімічні основи створення новітніх засобів для подолан...unclassified

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Peculiarities of interaction of malignant cells and tumors with chemotherapeutic nanocomposite remedies

Turanska,

Krupska,

Turov

et al. 2023

Poverhn.

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The purpose of the review is to find, generalize and analyze scientific data related to the specifics of the interaction of chemotherapeutic official drugs and nanocomposites based on them with malignant cells and tumors, primarily characterized by the emergence of drug resistance, the determination of promising directions and ways to overcome it, and the creation of new effective nanocomposite remedies for use in antitumor chemotherapy. The given data indicate the relevance of the topic. Targeted studies of the resistance of malignant cells and neoplasms to chemotherapeutic drugs have been carried out since the 1990s. The majority of works were performed according to the methodology, that involves the traditional use of chemotherapeutic drugs. In these works, the principle of the multifactorial nature of resistance was determined, the processes and mechanisms of its implementation were studied, related to the reduction of the accumulation of the chemotherapeutic drug in cells, the increase in the activity of detoxification systems, the strengthening of DNA repair processes, the reduction of apoptosis, and autophagy. A number of promising substances and influencing factors contributing to overcoming of resistance have been identified. However, the discovered ways to overcome the resistance of malignant cells and neoplasms to the corresponding drugs are at the stages of laboratory, preclinical, or, in the best case, clinical research. At the same time, it is not excluded that the use of the latest highly effective chemotherapeutic drugs will lead to the emergence of new mechanisms of resistance. Thus, at this time the results of research on the traditional use of chemotherapeutic drugs constitute a significant fundamental and practically important development regarding the determination of mechanisms of drug resistance, however, the problem of its medicinal overcoming remains far from being solved, and the used approaches give the impression of dead ends. With the development of nanotechnology, new scientific directions have been initiated and a significant amount of researches has been carried out, devoted to the creation and search for promising applications in oncology of nanocomposites based on bioinert, biocompatible and bioactive nanoparticle materials and modern chemotherapeutic drugs. It should be emphasized that all these works contain data indicating the advantages of introducing nanocomposite drugs into clinical practice, compared to the use of chemotherapeutic drugs in traditional forms. Against this background, purposeful researches are distinguished carried out by scientists of O.O. Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine and R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of National Academy of Sciences of Ukraine in the field of creation of modern polyfunctional nanocomposite chemotherapeutic agents for use in antitumor therapy, capable of overcoming drug resistance of malignant cells and neoplasms. So, at O.O. Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine for the first time magnetic fluids were synthesized containing antitumor drugs cisplatin, doxorubicin, gemcitabine, corresponding antibodies, their physicochemical properties were studied, and parameters for standardization were determined. At R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of National Academy of Sciences of Ukraine the antitumor properties of magnetic fluids were studied. On the basis of magnetic fluid with cisplatin, the first native magnetosensitive oncological drug "Feroplat" has been proposed, which has no analogues in the world. Feroplat is a standardized tool for increasing the effectiveness of chemotherapy and overcoming drug resistance of malignant neoplasms, intended for the delivery of cytostatics directly to the tumor tissue. This ensures its maximum entry into the cells and helps to increase the therapeutic effect. In order to introduce "Feroplat" into production and clinical practice, its preclinical tests were successfully performed. The analysis of the given data indicates the priority of works in the field of creation of new nanocomposite chemotherapeutic drugs for use in antitumor therapy, capable of overcoming drug resistance of malignant cells and neoplasms. The facts of overcoming the drug resistance of malignant neoplasms to cisplatin with the new native oncological drug "Feroplat", as well as high indicators of cytotoxic / cytostatic activity of nanobiocomposites based on saline solution, magnetite and cisplatin, doxorubicin, gemcitabine, etc., may indicate the fundamental need to change approaches to the use of modern antitumor chemotherapeutic agents – by replacing their traditional molecular forms with appropriate nanocomposite forms.

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Section: фізико-хімічні основи створення новітніх засобів для подолан...unclassified

Peculiarities of interaction of malignant cells and tumors with chemotherapeutic nanocomposite remedies

Turanska,

Krupska,

Turov

et al. 2023

Poverhn.

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Specific aspects of human immunoglobulin interactions with Fe3O4/≡Si(CH2)3NH2 nanocomposite surface

et al. 2023

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Hydration of bacterial lectin in native state and after immobilization on surface of hydrophobic silica

Turov¹,

Gorbyk²,

Krupska³

et al. 2022

Him. Fiz. Tehnol. Poverhni

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The aim of the work was to study the peculiarities of interaction of the surface of bacterial lectin of Bacillus subtilis IMB B-7724 inthe native state and under different model conditions with water molecules by 1 H NMR; to create a composite system based on the studied lectin, in which the protein molecule is minimally affected by the surface of the carrier, because protein molecules are capable to bind a significant amount of water localized in the spaces between the polymer chains. A method of “dry” immobilization of bacterial lectin on the surface of hydrophobic silica has been developed. Hydration of native lectin and lectin fixed on the surface of hydrophobic silica AM-1-175 was studied by low-temperature 1 H NMR spectroscopy. It has been shown that the immobilization of lectin on the surface of AM1 is accompanied by an increase in the interfacial energy gS from 4.1 to 5.2 J/g. This is due to an increase in the concentration of strongly bound water. Analysis of changes in the distributions of radii R of clusters of adsorbed water allows us to state that in water adsorbed by native lectin, there are two main maxima at R = 1 and 3 nm. In the immobilized state, the maximum at R = 1 nm is present in both types of water (of different order), but the second maximum is observed only for more ordered associates. Chloroform medium slightly reduces the binding energy of water to native lectin molecules (from 4.3 to 4.1 J/g), but in the case of immobilized lectin in CDCl3 medium, the value of ΣgS increases from 5.2 to 7.4 J/g. That is, the weakly polar medium promotes to increase in the interaction of water with interfaces, which is manifested in a relative increase in the number of water clusters of smaller size (Fig. 4). It should be noted that weakly associated forms of water (signal 3) are also represented by several types of clusters that have a radius in the range R = 1–10 nm, and their size distribution changes significantly during immobilization of lectin on the surface of AM1. Probably, weakly associated types of water are formed both in cavities, between polymer chains of protein molecules, and on the surface of AM1, free of protein.

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Antitumor vector systems based on bioactive lectin of Bacillus subtilis ІМВ B-7724

Cited by 7 publications

References 12 publications

Peculiarities of interaction of malignant cells and tumors with chemotherapeutic nanocomposite remedies

Peculiarities of interaction of malignant cells and tumors with chemotherapeutic nanocomposite remedies

Specific aspects of human immunoglobulin interactions with Fe3O4/≡Si(CH2)3NH2 nanocomposite surface

Hydration of bacterial lectin in native state and after immobilization on surface of hydrophobic silica

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