Antitumoural activity of Brazilian red propolis fraction enriched with xanthochymol and formononetin: An in vitro and in vivo study

Novak, Estela Maria; Silva, Martha Silveira e Costa; Marcucci, María Cristina; Sawaya, Alexandra Christine Helena Frankland; Lopez, Begoña G.C.; Fortes, Maria Angela Henriques Zanella; Giorgi, Ricardo Rodrigues; Marumo, Kamila Tamie; Rodrigues, Rosangela; Maria, Durvanei Augusto

doi:10.1016/j.jff.2014.09.008

Cited by 36 publications

(25 citation statements)

References 35 publications

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“…It has been reported that two of the ion markers of red propolis formononetin and xantochymol ( m/z 601) have cytotoxic activity (Novak et al . ); thus, they might be contributing to this effect in red propolis. However, regarding the antimicrobial activity, previous reports indicate that it is due to the synergic effect of the substances present in red propolis (Cabral et al .…”

Section: Discussionmentioning

confidence: 98%

“…The isoflavone formononetin and the prenylated benzophenone xantochymol, displayed cytotoxic activity by inhibiting the growth of different cancer cell lines, inducing apoptosis through activation of the caspase‐3‐dependent pathway (Novak et al . ), nevertheless, more studies on the cytotoxic activity in nontumour cell lines are needed, to evaluate the safety of the use of red propolis. This product is popularly consumed in Brazil but the population is unaware of its possible toxic effects.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and cytotoxic activity of red propolis: an alert for its safe use

et al. 2015

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Antimicrobial and cytotoxic activity of red propolis: an alert for its safe use

et al. 2015

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“…Isoflavonoids have been reported to possess antimicrobial, antifungal, anticancer and antioxidant activities (Alencar et al, 2007). Novak et al (2014), enriched a fraction of the red propolis extract with formononetin making it more active than the original extract for its cytotoxic activity .…”

Section: Discussionmentioning

confidence: 99%

Chemical markers and antifungal activity of red propolis from Sergipe, Brazil

Mendonça

Araujo

et al. 2015

Food Sci. Technol (Campinas)

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The aim of this study was to analyze the physicochemical properties and antifungal activities of the red propolis samples from Sergipe, Brazil, and also evaluate their variability throughout the year. The characterization of the hydroalcoholic extract (HPE) of the red propolis samples was performed monthly from Dctober 2009 to September 2010. The concentrations of the bioactive compounds varied during the year, but their chromatographic profiles were similar. Four compounds were identified by comparison with authentic standards. Formononetin was one of the predominant compounds in all propolis extracts. In our study, it was observed that all the propolis samples inhibited the growth of Candida species. Multivariate analysis confirmed the variations in chemical composition and color of the HPEs throughout the year. The biological activities of the HPEs were statistically significant (p<0.05), and all samples exhibited antifungal properties.

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“…Apoptosis suppression is recognized as a hallmark of cancer and may be a novel approach for the development of new antitumor drugs (Khan, Blanco‐Codesido, & Molife, ; Yaacoub, Pedeux, Tarte, & Guillaudeus, ). Propolis samples from Mexico, Brazil, Poland, Turkey, Iraq, China, Japan, and Thailand have shown apoptotic effects on cancer cells through activation of caspases 3, 6, 8, 9, and Bax, changes in the mitochondrial membrane potential (ΔΨm) and Bcl‐2 expression (Alday et al, ; Frión‐Herrera, Díaz‐García, Ruiz‐Fuentes, Rodríguez‐Sánchez, & Sforcin, ; Khacha‐Ananda, Tragoolpua, Chantawannakul, & Tragoolpua, ; Motomura et al, ; Novak et al, ; Seda Vatansever et al, ; Sulaiman et al, ; Xuan et al, ). In addition, reactive oxygen species (ROS) production may be involved in the effects of natural products on tumor cells (Shashi, Jaswant, Madhusudana, Kumar, & Nabi, ; Yan et al, ).…”

Section: Introductionmentioning

confidence: 99%

The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

Mora

Santiago

Conti

et al. 2018

Phytotherapy Research

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Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH‐DA. Transwell assay was used to evaluate the invasiveness of propolis‐treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.

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Antitumoural activity of Brazilian red propolis fraction enriched with xanthochymol and formononetin: An in vitro and in vivo study

Cited by 36 publications

References 35 publications

Antimicrobial and cytotoxic activity of red propolis: an alert for its safe use

Antimicrobial and cytotoxic activity of red propolis: an alert for its safe use

Chemical markers and antifungal activity of red propolis from Sergipe, Brazil

The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

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