Abstract:Paullinia pinnata Linn (Sapindaceae) is a
medicinal plant, locally used in the West Region of Cameroon for the treatment of
typhoid fever. This work was designed to evaluate the antityphoid and antioxidant
activities of the extracts and compounds of P.
pinnata.The methanol extracts of the leaves and stems were tested for antityphoid and
antioxidant activities. Compounds were isolated, and their structures elucidated by
analysis of spectroscopic data in conjuction with literature data and tested for the
same ac… Show more
“…The compounds isolated in the present study were formerly isolated from other plants and the biological activities of some were demonstrated [23, 24]. Besides, in our previous investigation, their antityphoid and antioxidant properties were demonstrated [9]. P. pinnata extract have been proven to possess antioxidant [25] and vascular relaxation [2] properties.Figure 1
Chemical structures of compounds from the leaves (1–3) and stems (4–7) of
P. pinnata.…”
Section: Resultsmentioning
confidence: 78%
“…As a result of our interest in the chemical and biological investigation of this plant, a methylinositol ( 1 ), steroidal terpenoids ( 2 and 4 ), and oleanane triterpenoids ( 3 , 5 – 7 ) were isolated from its leaf and stem methanol extracts. In our previous studies, the antityphoid and antioxidant properties of these compounds were evaluated [9]. The extracts, fractions and all the compounds were evaluated for their antimicrobial activities against eight bacteria, five yeasts and five dermatophytes species and the results are reported herein.…”
BackgroundPaullinia pinnata L. (Sapindaceae) is an African woody vine, which is widely used in traditional medicine for the treatment of human malaria, erectile dysfunction and bacterial infections. A phytochemical investigation of its methanol leaf and stem extracts led to the isolation of seven compounds which were evaluated for their antimicrobial properties.MethodsThe extracts were fractionated and compounds were isolated by chromatographic methods. Their structures were elucidated from their spectroscopic data in conjunction with those reported in literature. The antimicrobial activities of the crude extracts, fractions and compounds were evaluated against bacteria, yeasts and dermatophytes using the broth micro-dilution technique.ResultsSeven compounds: 2-O-methyl-L-chiro-inositol (1), β-sitosterol (2), friedelin (3), 3β-(β-D-Glucopyranosyloxy) stigmast-5-ene (4), (3β)-3-O-(2′-Acetamido-2′-deoxy-β-D-glucopyranosyl) oleanolic acid (5), (3β,16α-hydroxy)-3-O-(2′-Acetamido-2′-deoxy-β-D-glucopyranosyl) echinocystic acid (6) and (3β)-3-O-[β-D-glucopyranosyl-(1″-3′)-2′-acetamido-2′-deoxy-β-D-galactopyranosyl]oleanolic acid (7) were isolated. Compounds 5 and 7 showed the best antibacterial and anti-yeast activities respectively (MIC value range of 0.78-6.25 and 1.56-6.25 μg/ml), while 6 exhibited the best anti-dermatophytic activity (MIC value range of 6.25-25 μg/ml).ConclusionThe results of the present findings could be considered interesting, taking into account the global disease burden of these susceptible microorganisms, in conjunction with the search for alternative and complementary medicines.
“…The compounds isolated in the present study were formerly isolated from other plants and the biological activities of some were demonstrated [23, 24]. Besides, in our previous investigation, their antityphoid and antioxidant properties were demonstrated [9]. P. pinnata extract have been proven to possess antioxidant [25] and vascular relaxation [2] properties.Figure 1
Chemical structures of compounds from the leaves (1–3) and stems (4–7) of
P. pinnata.…”
Section: Resultsmentioning
confidence: 78%
“…As a result of our interest in the chemical and biological investigation of this plant, a methylinositol ( 1 ), steroidal terpenoids ( 2 and 4 ), and oleanane triterpenoids ( 3 , 5 – 7 ) were isolated from its leaf and stem methanol extracts. In our previous studies, the antityphoid and antioxidant properties of these compounds were evaluated [9]. The extracts, fractions and all the compounds were evaluated for their antimicrobial activities against eight bacteria, five yeasts and five dermatophytes species and the results are reported herein.…”
BackgroundPaullinia pinnata L. (Sapindaceae) is an African woody vine, which is widely used in traditional medicine for the treatment of human malaria, erectile dysfunction and bacterial infections. A phytochemical investigation of its methanol leaf and stem extracts led to the isolation of seven compounds which were evaluated for their antimicrobial properties.MethodsThe extracts were fractionated and compounds were isolated by chromatographic methods. Their structures were elucidated from their spectroscopic data in conjunction with those reported in literature. The antimicrobial activities of the crude extracts, fractions and compounds were evaluated against bacteria, yeasts and dermatophytes using the broth micro-dilution technique.ResultsSeven compounds: 2-O-methyl-L-chiro-inositol (1), β-sitosterol (2), friedelin (3), 3β-(β-D-Glucopyranosyloxy) stigmast-5-ene (4), (3β)-3-O-(2′-Acetamido-2′-deoxy-β-D-glucopyranosyl) oleanolic acid (5), (3β,16α-hydroxy)-3-O-(2′-Acetamido-2′-deoxy-β-D-glucopyranosyl) echinocystic acid (6) and (3β)-3-O-[β-D-glucopyranosyl-(1″-3′)-2′-acetamido-2′-deoxy-β-D-galactopyranosyl]oleanolic acid (7) were isolated. Compounds 5 and 7 showed the best antibacterial and anti-yeast activities respectively (MIC value range of 0.78-6.25 and 1.56-6.25 μg/ml), while 6 exhibited the best anti-dermatophytic activity (MIC value range of 6.25-25 μg/ml).ConclusionThe results of the present findings could be considered interesting, taking into account the global disease burden of these susceptible microorganisms, in conjunction with the search for alternative and complementary medicines.
“…The reduction in the bacterial burden of faeces in infected and untreated rats may be explained by the protective action of their immune system against the pathogens (Lunga et al, 2014). The in vitro and in vivo analyses of antibacterial activity revealed the antibacterial and therapeutic potential of A. leiocarpa.…”
The present study was conducted to investigate in vitro and in vivo antibacterial activity of the root bark extracts of Anogeissus leiocarpa (DC) Guill. & Perr, in Escherichia coli extended-spectrum βlactamases (ESBL)-induced diarrhea in rat. The antibacterial activity was performed in vitro by determining the inhibition zone using standard agar diffusion method as well as in vivo on E. coli infected Wistar rat model. Both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were carried through microdilution method. Results obtained in this study indicated that ethanolic and acetatic extracts were only active on bacteria presenting an inhibition zone range from 8 to 16 mm. The MIC observed in agar slant tubes ranged from 6.25 to 50 mg/ml. The ethanolic fraction of A. leiocarpa (ETHA) showed the highest in vitro antibacterial activity against strains with MICs ranging from 6.25 to 12.5 mg/ml and MBCs ranging from 12.5 to 25 mg/ml. In vivo, after infection, diarrhea increased faeces frequency, weight and volume faeces and bacterial faeces load to a maximum on the 2nd day after infection (P < 0.01). ETHA normalized the appearance, weight, volume and water content of faeces. To all doses, like ciprofloxacin it reduced significantly (P < 0.05) the bacterial growth compared to control Lot (infected and untreated). The death rate in diarrheic control Lot was 50% by Day 14. No death was recorded in Lot treated with ciprofloxacin and Lot treated with ethanolic fraction at dose of 2222.22 mg/kg body weight (bw). This study supports the use of A. leiocarpa in the traditional treatment of bacterial infections and offer many perspectives in the search for new molecules against resistant microbial strains.
“…The presence of these groups of compounds in the methanol extract of P. pinnata could explain the dose-dependent periodic eradication of typhoid fever and thus the weight gain observed in this study. Besides, many compounds from this extract were isolated; including oleanane-type triterpenoid saponins and a steroidal saponin with remarkable antisalmonella activities in vitro 4 . This phenomenon was further enhanced by the eradication of the disease condition since it was found that typhoid fever induces weight loss.…”
Section: Discussionmentioning
confidence: 99%
“…The antioxidant properties of the methanol extract of the leaves and antibacterial activity of fatty acids from the roots of P. pinnata have been investigated 2,3 . In the ongoing studies, potential antityphoid properties of the methanol extract from the leaves of P. pinnata, both in vitro on four species of Salmonella (S. typhi, S. paratyphi A, S. paratyphi B and S. typhimurium) and in vivo on a S. typhimurium-induced typhoid model in Wistar rats was demonstrated 4 . In vitro, the Minimum Inhibitory Concentrations (MICs) ranged from 48-3125 μg mLG 1 , while in vivo, the extract could eradicate the disease condition after 4 and 5 days, respectively at a daily oral dose of 446 and 223 mg kgG 1 b.wt.…”
Background and Objective: Paullinia pinnata is an African woody vine whose leaf decoction has been used in Cameroon for the treatment of bacterial infections like typhoid fever, syphilis, gonorrhea, diarrhoea and symptoms such as stomach-ache and waist pain. The present study was designed to evaluate the adverse side effects resulting from the use of P. pinnata methanol leaf extract in the treatment of Salmonella typhimurium-induced typhoid in Wistar rats. Methodology: After the establishment of infection by oral administration of a S. typhimurium suspension, animals were treated by the daily administration of P. pinnata methanol leaf extract at various doses (55.75, 111.50, 223 and 446 mg kgG 1 b.wt.). The effect of the extract on body weight evolution was monitored daily. Results: Also, the effect of the extract on relative organ weight, biochemical parameters as well as liver histology was assessed. Irrespective of sex, typhoid fever induced an abnormal increase in the relative weight of most vital organs of toxicological importance. However, extract treatment normalized the excessive increase in relative organ weights; while inducing a significant (p<0.05) body weight gain in a dose-dependent manner. The level of liver enzymes (ALT and AST) were significantly (p<0.05) reduced after the treatment of the animals with P. pinnata leaf extract. The histopathological analysis of the liver revealed that extract treatment greatly reduced the degree of liver affections in a dose-dependent manner. However, at high doses (dose $223 mg kgG 1 b.wt.) the extract was capable of stimulating hepatic necrosis. Conclusion: The overall results of this study indicate that the methanolic extract of P. pinnata leaves has hepatoprotective effects especially in males. It has adverse side effects at high doses and even at low doses in female rats and thus, should be used with caution in male and should probably be eliminated from the treatment of female subjects.
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