Antiviral effect of the egg wax of Amblyomma cajennense (Acari: Ixodidae)

Abstract

The control of viral infections,

In-vitro anticandidial efficacy of tick egg wax from Hyalomma marginatum, Rhipicephalus bursa and Dermacentor marginatus

“…In the studies carried out to determine the antimicrobial activities of the wax, successful results have been obtained in various degrees against different agents such as Escherichia coli (22,29), Staphylococcus epidermidis (25)(26)(27)32), Staphylococcus aureus (22,26,29), Serratia marcescens (25), Bacillus cereus (26), Bacillus subtilis (25)(26)(27), Micrococcus luteus (27,29,30), Pseudomonas aeruginosa (22,31), Enterecoccus faecalis, Enterecoccus faecium, Nocardia asteroides, Salmonella enteritidis, Klebsiella pneumonia (22), picornavirus (28), and influenza virus (28,29). Furthermore, the wax of Boophilus microplus was reported to be effective on C. albicans to some extent (30), and the wax of Rhipicephalus sanguineus was found to be effective on the same agent (29).…”

“…In the previous studies, the egg waxes of Amblyomma habreum (25,26) A. cajennense (27)(28)(29), A. aureolatum (29), Boophilus (Rhipicephalus) microplus (29-32), Rhipicephalus sanguineus (29), Haemaphysalis longicornis, H. doenitzi, Dermacentor silvarum and Hyalomma asiaticum (22) have been tested against various bacteria, fungi, and viruses, and some considerable results were obtained. However, these studies have also revealed that the antimicrobial efficacy of the wax varies dramatically related to the tick species.…”

Antiviral activity of the hemolymph ofPodalia sp and M. albicolis(Lepidoptera: Megalopigydae)

“…Other peptide, isolated from a marine sponge Petromica citrine, has showed an antiviral activity of against bovine viral diarrhea vírus (Bastos et al, 2013). Lima-Netto et al, 2012, observed the antiviral effect of eggs wax from tick Amblyomma cajennense against influenza and picornavirus, for influenza virus, in this case, an amount as small as 12 μg/mL from a crude suspension of egg wax was able to reduce in 128 UHA (hemaglutinant unit) of influenza virus (H1N1). With picornavirus, this reduction was of 256-fold.…”

“…Carmo et al, 2012) describes a system for the protein expression in Sf9/baculovirus cells using the recombinant DNA to obtain a protein from the L. obliqua caterpillar that displays a potent antiviral action obtained by Greco et al, 2009. Recently, our group has studied the presence of antiviral agents in diferents animals source (Lima-Netto et al, 2012., Carmo et al, 2015, Toledo-Piza et al, 2016, 2018; Carvalho et al, 2017; Coelho et al, 2015, 2018). So, the objectives of this work were the identification and isolation of antiviral substances in hemolymph from Podalia sp and M. Albicolis ( Lepidoptera: Megalopigydae )…”

Clonagem e expressão de proteína antiviral presente na hemolinfa de Lonomia obliqua por tecnologia de DNA recombinante em Escherichia coli.

“…Recentemente, nosso grupo tem demonstrado a presença de proteínas com alto potencial antiviral e antimicrobiano na cera de ovos de carrapatos Amblyomma cajennense (DE LIMA-NETTO et al, 2012;LIMA-NETTO et al, 2011) e na hemolinfa de Lonomia obliqua (GRECO et al, 2009). Cultivos de células de inseto suplementado com hemolinfa de L. obliqua tiveram sua longevidade aumentada (MARANGA et al, 2003).…”