Antiviral Efficacy of Citra-kill<sup>®</sup>, Disinfectant Solution Against Avian Influenza Virus

Objectives: This study evaluated the virucidal efficacy against avian influenza virus (AIV) of a disinfectant spray containing 0.25% grapefruit seed extract, 0.2% citric acid, 0.0625% malic acid and 0.0125% benzalkonium chloride. Methods:The disinfectant spray was diluted several times with hard water (HW) and organic matter (OM). Two point five mL of each diluent was added into each test tube, and 2.5 mL of AIV suspension was inserted into each test tube. After 30 minutes of virus-disinfectant contact reaction at 4 o C, 2.5 mL of 10% inactivated fetal bovine serum was added into each test tube to neutralize the sanitizer efficacy. The neutralized solutions were serial 10-fold dilutions with phosphate buffer solution, and 0.2 mL of the diluents was injected into the allantoic cavity of five ten-day-old-chickens per dilution time. After incubation of the embryos for five days, the viability of the AIV was examined by hemagglutination titer. The valid dilution of the disinfectant spray was estimated according to the dilution time that the virus titer was inactivated more than 10 4 50% egg-infective dose (EID50)/ mL compared with pathogen control. three-fold dilutions, respectively. The AIV titer of the pathogen control was more than 6.1 log10EID50/mL, and there was no embryonic toxicity. Conclusion:The present study showed that this disinfectant spray has effective virucidal activity against AIV.

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Biocidal effect to fish pathogens of Aqua farmsafe^®composed of yucca extract and didecyldimethylammonium chloride

Seo¹,

Jeon²,

Hwang³

et al. 2013

Journal of fish pathology

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In this study, the disinfectant efficacy of Aqua farmsafe ® , composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4℃. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe ® possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe ® against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua framsafe ® will be able to reduce fish diseases.

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Antiviral Efficacy of Citra-kill^®, Disinfectant Solution Against Avian Influenza Virus

Cited by 3 publications

References 11 publications

Virucidal efficacy of a disinfectant solution composed of citric acid, malic acid and phosphoric acid against avian influenza virus

Virucidal efficacy of a disinfectant solution composed of citric acid, malic acid and phosphoric acid against avian influenza virus

Virucidal Efficacy against Avian Influenza Virus of a Disinfectant Spray Containing Grapefruit Seed Extracts, Citric Acid, Malic Acid and Benzalkonium Chloride

Biocidal effect to fish pathogens of Aqua farmsafe^®composed of yucca extract and didecyldimethylammonium chloride

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Antiviral Efficacy of Citra-kill®, Disinfectant Solution Against Avian Influenza Virus

Cited by 3 publications

References 11 publications

Virucidal efficacy of a disinfectant solution composed of citric acid, malic acid and phosphoric acid against avian influenza virus

Virucidal efficacy of a disinfectant solution composed of citric acid, malic acid and phosphoric acid against avian influenza virus

Virucidal Efficacy against Avian Influenza Virus of a Disinfectant Spray Containing Grapefruit Seed Extracts, Citric Acid, Malic Acid and Benzalkonium Chloride

Biocidal effect to fish pathogens of Aqua farmsafe®composed of yucca extract and didecyldimethylammonium chloride

Contact Info

Product

Resources

About

Antiviral Efficacy of Citra-kill^®, Disinfectant Solution Against Avian Influenza Virus

Biocidal effect to fish pathogens of Aqua farmsafe^®composed of yucca extract and didecyldimethylammonium chloride