APOBEC3G Inhibits MicroRNA-mediated Repression of Translation by Interfering with the Interaction between Argonaute-2 and MOV10

Liu, Chao; Zhang, Xue; Huang, Feng; Yang, Bin; Li, Jun; Liu, Bingfeng; Luo, Haihua; Zhang, Ping; Zhang, Hui

doi:10.1074/jbc.m112.354001

Cited by 46 publications

(66 citation statements)

References 44 publications

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“…Steps are numbered in small circles. Review against L1 mobilization include the RNA helicase MOV10 (which degrades L1 mRNAs or suppresses their translation) 24 and APOBEC3 family members (Figure 1b). 25 Different members of the APOBEC3 family may inhibit L1 retrotransposition by different mechanisms.…”

Section: Structure and Retrotransposition Process Of L1mentioning

confidence: 99%

“…miRNAs and lncRNAs may regulate gene expression directly or indirectly through competing endogenous RNA (ceRNA) networks. 68 In normal somatic cells, L1 retrotransposition is suppressed 22,31 and L1 transcripts are degraded or processed into regulatory RNAs that maintain cell homeostasis, 19,21,24,66,67 whereas in cancer, one study found that L1 transcripts were potentially biased toward cDNA formation and retrotransposition, 69 leading to speculation that deregulated regulatory RNA networks may drive tumorigenesis.…”

Section: Functions Of L1s In Cancer Retrotransposition-dependent Funcmentioning

confidence: 99%

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LINE-1 in cancer: multifaceted functions and potential clinical implications

Han

et al. 2016

Genetics in Medicine

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ReviewThe human genome is abundant with interspersed repetitive sequences originated from retrotransposons. Until now, three categories of retrotransposons have remained unequivocally active: LINE-1(L1), Alu, and SVA elements. The first one is autonomous-capable of self-propagation through RNA intermediates-and the latter two are nonautonomous and thus rely on L1 for mobilization. There are approximately 500,000 L1 copies in the human genome, composing 17% of human DNA.

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Section: Structure and Retrotransposition Process Of L1mentioning

confidence: 99%

Section: Functions Of L1s In Cancer Retrotransposition-dependent Funcmentioning

confidence: 99%

LINE-1 in cancer: multifaceted functions and potential clinical implications

Han

et al. 2016

Genetics in Medicine

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“…The sequences of MOV10-specific small interfering RNAs (siRNAs) (SMARTpool) were described previously (31). MOV10-specific siRNAs and control siRNA were synthesized by Ribobio (Guangzhou, China).…”

mentioning

confidence: 99%

“…The cells were placed into 60-mm plates and further transfected with MOV10-HA-and NP-FLAG-expressing plasmids. Procedures were followed as previously described (31).…”

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confidence: 99%

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Host Protein Moloney Leukemia Virus 10 (MOV10) Acts as a Restriction Factor of Influenza A Virus by Inhibiting the Nuclear Import of the Viral Nucleoprotein

Zhang

Huang

Tan

et al. 2016

J Virol

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The viral ribonucleoprotein (vRNP) complex of influenza A viruses (IAVs) contains an RNA-dependent RNA polymerase complex (RdRp) and nucleoprotein (NP) and is the functional unit for viral RNA transcription and replication. The vRNP complex is an important determinant of virus pathogenicity and host adaptation, implying that its function can be affected by host factors. In our study, we identified host protein Moloney leukemia virus 10 (MOV10) as an inhibitor of IAV replication, since depletion of MOV10 resulted in a significant increase in virus yield. MOV10 inhibited the polymerase activity in a minigenome system through RNA-mediated interaction with the NP subunit of vRNP complex. Importantly, we found that the interaction between MOV10 and NP prevented the binding of NP to importin-␣, resulting in the retention of NP in the cytoplasm. Both the binding of MOV10 to NP and its inhibitory effect on polymerase activity were independent of its helicase activity. These results suggest that MOV10 acts as an anti-influenza virus factor through specifically inhibiting the nuclear transportation of NP and subsequently inhibiting the function of the vRNP complex. IMPORTANCEThe interaction between the influenza virus vRNP complex and host factors is a major determinant of viral tropism and pathogenicity. Our study identified MOV10 as a novel host restriction factor for the influenza virus life cycle since it inhibited the viral growth rate. Conversely, importin-␣ has been shown as a determinant for influenza tropism and a positive regulator for viral polymerase activity in mammalian cells but not in avian cells. MOV10 disrupted the interaction between NP and importin-␣, suggesting that MOV10 could also be an important host factor for influenza virus transmission and pathogenicity. Importantly, as an interferon (IFN)-inducible protein, MOV10 exerted a novel mechanism for IFNs to inhibit the replication of influenza viruses. Furthermore, our study potentially provides a new drug design strategy, the use of molecules that mimic the antiviral mechanism of MOV10.

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Genomic Accumulation of Retrotransposons Was Facilitated by Repressive RNA‐Binding Proteins: A Hypothesis

Attig

Ule

2019

BioEssays

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Retrotransposon‐derived elements (RDEs) can disrupt gene expression, but are nevertheless widespread in metazoan genomes. This review presents a hypothesis that repressive RNA‐binding proteins (RBPs) facilitate the large‐scale accumulation of RDEs. Many RBPs bind RDEs in pre‐mRNAs to repress the effects of RDEs on RNA processing, or the formation of inverted repeat RNA structures. RDE‐binding RBPs often assemble on extended, multivalent binding sites across the RDE, which ensures repression of cryptic splice or polyA sites. RBPs thereby minimize the effects of RDEs on gene expression, which likely reduces the negative selection against RDEs. While mutations that change splice sites in RDEs act as an off‐on switch in exon formation, mutations that decrease the multivalency of RBP binding sites resemble a rheostat that enables a more gradual evolution of new RDE‐derived exons. RBPs might also repress aberrant processing of active retrotransposons, thus increasing the chance that full‐length copies are made. Taken together, in this review, it is proposed that RBPs facilitate the widespread accumulation of intronic RDEs by repressing RNA processing while chaperoning their potential to gradually evolve into new exons.

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APOBEC3G Inhibits MicroRNA-mediated Repression of Translation by Interfering with the Interaction between Argonaute-2 and MOV10

Cited by 46 publications

References 44 publications

LINE-1 in cancer: multifaceted functions and potential clinical implications

LINE-1 in cancer: multifaceted functions and potential clinical implications

Host Protein Moloney Leukemia Virus 10 (MOV10) Acts as a Restriction Factor of Influenza A Virus by Inhibiting the Nuclear Import of the Viral Nucleoprotein

Genomic Accumulation of Retrotransposons Was Facilitated by Repressive RNA‐Binding Proteins: A Hypothesis

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