Apolipoprotein AI Deficiency Inhibits Serum Opacity Factor Activity against Plasma High Density Lipoprotein via a Stabilization Mechanism

Rosales, Corina; Patel, Niket; Gillard, Baiba K.; Yelamanchili, Dedipya; Yang, Yaliu; Courtney, Harry S.; Santos, Raul D.; Gotto, Antonio M.; Pownall, Henry J.

doi:10.1021/bi501486z

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…It plays important roles in preventing lipid accumulation in tissues and in maintaining cholesterol dynamic balance [ 17 , 18 ]. Genetic deficiency in Apo A-I has been associated with excessive cholesterol accumulation in human and poultry [ 19 – 21 ]. Association studies showed that a single nucleotide polymorphism (SNP) upstream of the ATG initiation codon of the chicken Apo A-I gene was associated with abdominal fat weight and abdominal fat percentage [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

Liu

Wang

et al. 2016

Proteome Sci

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BackgroundThe molecular mechanism underlying broiler fat deposition is still poorly understood.MethodCurrently, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in abdominal adipose tissues of birds at 4 week of age derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF).ResultsThirteen differentially expressed protein spots were screened out and identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The protein spots were matched to thirteen proteins by searching against the NCBInr database. These identified proteins were apolipoprotein A-I (Apo A-I), cytokeratin otokeratin, ATP synthase subunit alpha, peptidyl-prolyl cis-trans isomerase FKBP4 (PPIase FKBP4), aspartate aminotransferase, carbonic anhydrase II (CA-II), prostaglandin-H2 D-isomerase precursor, fibrinogen alpha chain, lamin-A (LMNA), superoxide dismutase [Mn] (MnSOD), heat shock protein beta-1 (HSPβ1) and two predicted proteins. These differentially expressed proteins are involved mainly in lipid metabolism, amino acid metabolism, signal transduction, energy conversion, antioxidant, and cytoskeleton. Differential expression of Apo A-I, PPIase FKBP4, and cytokeratin otokeratin proteins were further confirmed by Western blot analysis. Quantitative real-time RT-PCR analyses showed that, of these 13 differentially expressed proteins, only PPIase FKBP4 and cytokeratin otokeratin were differentially expressed at mRNA level between the two lines.ConclusionsOur results have provided further information for understanding the basic genetics control of growth and development of broiler adipose tissue.

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Section: Discussionmentioning

confidence: 99%

Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

Liu

Wang

et al. 2016

Proteome Sci

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“…[32] The kinetics of the SOF reaction with HDL and acylated HDL were determined by right angle light scattering in real time and according to the distribution of reaction products by SEC as described. [23, 33] Briefly, HDL (0.27 mg/mL in 3 mL) was placed in a fluorescence cuvette, which was transferred to the cell compartment of an Aviv spectrofluorimeter. After equilibration to 37 °C, SOF (2.8 μg/2.5 μL) was added with constant stirring and the light scattering intensity (325 nm) monitored at 90° to the incident light.…”

Section: Methodsmentioning

confidence: 99%

Acylation of lysine residues in human plasma high density lipoprotein increases stability and plasma clearance in vivo

Yang

Rosales

Gillard

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Although human plasma high density lipoproteins (HDL) concentrations negatively correlate with atherosclerotic cardiovascular disease, underlying mechanisms are unknown. Thus, there is continued interest in HDL structure and functionality. Numerous plasma factors disrupt HDL structure while inducing the release of lipid free apolipoprotein (apo) AI. Given that HDL is an unstable particle residing in a kinetic trap, we tested whether HDL could be stabilized by acylation with acetyl and hexanoyl anhydrides, giving AcHDL and HexHDL respectively. Lysine analysis with fluorescamine showed that AcHDL and HexHDL respectively contained 11 acetyl and 19 hexanoyl groups. Tests with biological and physicochemical perturbants showed that HexHDL was more stable than HDL to perturbant-induced lipid free apo AI formation. Like the reaction of streptococcal serum opacity factor against HDL, the interaction of HDL with its receptor, scavenger receptor class B member 1 (SR-B1), removes CE from HDL. Thus, we tested and validated the hypothesis that selective uptake of HexHDL-[3H]CE by Chinese hamster ovary cells expressing SR-B1 is less than that of HDL-[3H]CE; thus, selective SR-B1 uptake of HDL-CE depends on HDL instability. However, in mice, plasma clearance, hepatic uptake and sterol secretion into bile were faster from HexHDL-[3H]CE than from HDL-[3H]CE. Collectively, our data show that acylation increases HDL stability and that the reaction of plasma factors with HDL and SR-B1-mediated uptake are reduced by increased HDL stability. In vivo data suggest that HexHDL promotes charge-dependent reverse cholesterol transport, by a mechanism that increases hepatic sterol uptake via non SR-B1 receptors, thereby increasing bile acid output.

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“…The rate-limiting step of the SOF reaction is apo AI desorption from the HDL surface [4–6]. The SOF reaction mechanism was revealed by chemical kinetic studies of its reaction vs. HDL [2] and by cryo electron microscopy [4]; SOF is a fusogen that binds a “host” HDL with a high affinity binding site and a “guest” HDL with a lower affinity delipidation site.…”

Section: Introductionmentioning

confidence: 99%

Structural Stability of Streptococcal Serum Opacity Factor

et al. 2017

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Streptococcal serum opacity factor (SOF) is a protein that clouds the plasma of multiple mammalian species by disrupting high density lipoprotein (HDL) structure. Intravenous infusion of low dose SOF (4 μg) into mice reduces their plasma cholesterol concentrations ~40% in 3 h. Here we investigated the effects of pH, ionic strength, temperature, and denaturation with guanidinium chloride (GdmCl) on SOF stability and its reaction vs. HDL. SOF stability was tested by pre-incubation of SOF at various temperatures, pH’s, and GdmCl concentrations and measuring the SOF reaction rate at pH 7.4 and 37 °C. SOF retained activity at temperatures up to 58 °C, at pH = 4 to 10, and in 8.5 M GdmCl. The effects of GdmCl, pH, and ionic strength on the SOF reaction rates were also measured. SOF was inactive at GdmCl ≥1 M; SOF was most active at pH = 5, near its isoelectric point and at an ionic strength of 3 (in NaCl). These data reveal that SOF is a stable protein and suggest that its activity is determined, in part, by the effects of pH and ionic strength on its overall charge relative to that of its reaction target, HDL.

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Apolipoprotein AI Deficiency Inhibits Serum Opacity Factor Activity against Plasma High Density Lipoprotein via a Stabilization Mechanism

Cited by 5 publications

References 37 publications

Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

Acylation of lysine residues in human plasma high density lipoprotein increases stability and plasma clearance in vivo

Structural Stability of Streptococcal Serum Opacity Factor

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