Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line

Abstract

Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway.

Evaluation of the Effect of Five Colombian Propolis Extracts on the Expression of Genes Associated with Cell Cycle and Inflammation in a Canine Osteosarcoma Cell Line

“…[40] There is no information available on the ethnopharmacological activity of Colombian propolis associated with the antitumor effect, except for our own previous reports in which it was shown that EEP induced early and late apoptosis, related to the alteration of mitochondrial membrane potential, likewise, a significant decrease in the invasion, in canine osteosarcoma cells. [41,42] In the first place, the characterization consisted in evaluating proliferation, the exponential phase was established between 72 and 96 hr, a period of time in which the cell confluence was close to 70%, which could suggest that contact stimulates cell growth. These results coincide with tests carried out in the characterization of primary culture fibroblasts cultured with 10% SFB by other authors.…”