Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum

Abstract

Penicillium expansum 3.5425 was applied in solid-state fermentation (SSF) of agricultural wastes for polygalacturonase biosynthesis. Among various carbon additives, apple pomace was most suitable for the biosynthesis of polygalacturonase (1440.57 U/g). Optimization of medium parameters using rotational orthogonal design (ROD) experiment combined with optimal fermentation conditions resulted in a 2.72-fold increase in the polygalacturonase production. By using ammonium sulphate precipitation, ion-exchange and gel-permeation chromatography, the polygalacturonase produced by P. expansum 3.5425 was finally purified which had specific activity of 19269 U/mg and molecular weight of 30 kDa. The enzyme was remarkably active in the pH range of 3-5 and at 50°C, which makes it more acceptable in the industrial application. Besides, partially purified polygalacturonase (875.15 U/mL) was used for apple juice clarification and the clarity at 0.4 mL/kg was maximum, which reveals a great potential of polygalacturonase in food industry.

Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach

“…SSF is usually employed for filamentous fungi the PG activity of which is generally several times higher than that from yeasts. The fermentation of orange peel by an Aspergillus sujae strain, for 8 days, lead to 145 U mL −1 PG activity, and the fermentation of apple pomace by a Penicillium expansum strain, for four days, achieved 1103 U mL −1 PG activity [ 26 , 48 ]. Even yeasts, after longer fermentations, were able to generate higher PG activities when compared to the present study.…”

Chitinase activity potential and identification of chitinolytic bacteria isolated of swimmer crab’s cell

“…Moreover the ability of the bacteria to produce chitinase highly varied. Factors such as different types of bacteria, the growth rate of each isolate in the medium, temperature, pH or laboratory treatment during the experiment can be factors that influence variation in the produced enzyme activity [13,14] . Chitinase activity was 0.213 and 0.219 U/mL respectively of PBK 2 and SA 1.2 isolates from shrimp waste.…”