Application of a biomarker battery for the evaluation of the sublethal effects of pollutants in the earthworm Eisenia andrei

Gastaldi, Laura; Ranzato, Elia; Caprì, Flavia; Hankard, Peter K.; Pérès, Guénola; Canesi, Laura; Viarengo, Aldo; Pons, G.

doi:10.1016/j.cbpc.2007.04.014

Cited by 44 publications

(45 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Neutral red retention assay has been used to assess concentration-response relationships for a variety of metals and organic compounds (Svendsen et al, 2004;Gastaldi et al, 2007). Few studies, however, showed effects on neutral red retention time in earthworms exposed to chlorpyrifos contaminated soils (Hodge et al, 2000;Casabé et al, 2007;Reinecke & Reinecke, 2007).…”

Section: Resultsmentioning

confidence: 99%

Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters

Piola

Fuchs

Oneto

et al. 2009

Pesq. agropec. bras.

View full text Add to dashboard Cite

-The objective of this work was to evaluate the effects of chlorpyrifos on earthworms and on soil functional parameters. An integrated laboratory-fi eld study was performed in a wheat fi eld in Argentina, sprayed with chlorpyrifos at two recommended application rates (240 or 960 g ha -1 a.i.). Laboratory tests included neutral red retention time, comet assay (single cell gel electrophoresis), and avoidance behavior, each using the earthworm Eisenia andrei exposed in soil collected 1 or 14 days after pesticide application, and the bait-lamina test. Field tests assessed organic matter breakdown using the litterbag and bait-lamina assays. Earthworm populations in the fi eld were assessed using formalin application and hand-sorting. The neutral red retention time and comet assays were sensitive biomarkers to the effects of chlorpyrifos on the earthworm E. andrei; however, the earthworm avoidance test was not suffi ciently robust to assess these effects. Feeding activity of soil biota, assessed by the bait lamina test, was signifi cantly inhibited by chlorpyrifos after 97 days, but recovered by the 118 th day of the test. Litterbag test showed no signifi cant differences in comparison to controls. Earthworm abundance in the fi eld was too low to adequately test the sensitivity of this assessment endpoint.Index terms: Eisenia andrei, agricultural soil, fi eld assay, laboratory bioassay. Biomarcadores para a avaliação dos efeitos de clorpirifós em minhocas e em parâmetros funcionais do soloResumo -O objetivo deste trabalho foi avaliar os efeitos do clorpirifós sobre as minhocas e sobre parâmetros funcionais do solo. Foi executado um estudo integrado campo-laboratório, em uma plantação de trigo na Argentina, onde foi aplicado clorpirifós em duas doses recomendadas (240 ou 960 g ha -1 a.i.). Os ensaios laboratoriais incluíram tempo de retenção do vermelho-neutro, ensaio cometa (eletroforese em gel de célula única) e teste de fuga, cada um com a minhoca Eisenia andrei exposta aos solos coletados 1 e 14 dias após tratamentos, e teste com a lâmina-isca. Nos bioensaios de campo, avaliou-se a decomposição da matéria orgânica em sacolas com alfafa e com a lâmina-isca. As populações de minhocas foram avaliadas no campo com uso do método de extração com formalina e remoção manual. O tempo de retenção do vermelho-neutro e o ensaio cometa foram biomarcadores sensíveis aos efeitos do clorpirifós na minhoca E. andrei; porém, o comportamento de fuga não foi efi ciente para avaliar tais efeitos. A atividade alimentar da biota do solo, avaliada pelo teste de lâmina-isca, foi signifi cativamente inibida pelo clorpirifós após 97 dias, mas recuperou-se no 118 o dia do teste. O teste de sacolas com alfafa não mostrou diferenças signifi cativas em comparação aos controles. A abundância das minhocas em campo foi muito baixa, para testar adequadamente a sensibilidade desta variável.Termos para indexação: Eisenia andrei, solos agrícolas, testes de campo, bioensaios de laboratório.

show abstract

Section: Resultsmentioning

confidence: 99%

Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters

Piola

Fuchs

Oneto

et al. 2009

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…The biochemical biomarkers are being utilized these days for the analysis of pollutant toxicity, metabolization and detoxification in earthworm [13] [14] which later can be used for the detection and assessment of contaminant that influence the environmental modifications [15].…”

Section: Introductionmentioning

confidence: 99%

Enzymes of Earthworm as Indicators of Pesticide Pollution in Soil

Tiwari¹,

Singh²,

Pandey³

et al. 2016

AER

View full text Add to dashboard Cite

The importance of the earthworms in the agricultural practices is well known. The increasing applications of pesticides and chemicals in the agricultural farms have adversely influenced the flora and fauna of the soil. Earthworms which immensely contribute in increasing the quality and fertility of agricultural soil are reported be worst hit organisms under such conditions. Recent reports have indicated growing interests among researchers to explore biochemical and molecular markers as indicators of accumulation of pollutants in the soil in general and pesticides in particular. The varying levels of several biomolecules in different parts of the earthworm have been reported which are indicative of sensitivity of the organisms to different xenobiotics. However, the existing information lacks the literature displaying stock of information regarding the impact of pesticides on the levels of some key enzymes regulating many crucial functions in the earthworm at one place. Keeping in view this issue, it was envisaged to bring out a mini review which illustrates updated information available on the impact of pesticides on the activities of certain key enzymes reported to be responsible for catalysing metabolic pathways concerning the neurotransmission system, energy metabolism, oxidative stress and amino acids metabolism in different body parts of the earthworms, a prospective bioindicators of pesticides contamination in the soil.

show abstract

“…Responses of the lysosomal system are generally thought to provide a first answer to pollutant exposure in a wide variety of animals including earthworms, since injurious lysosomal reactions frequently precede cell and tissue pathology (Moore et al, 2006). The neutral red retention assay (NRRA) has been successfully applied for the in vivo evaluation of lysosomal membrane stability in earthworm coelomocytes ScottFordsmand et al, 1998;Svendsen et al, 2004, Gastaldi et al, 2007. The quantification of this biomarker is based on the time at which 50% of the cells, previously incubated with neutral red, show sign of lysosomal leaking (the cytosol becoming red and the cells rounded), as evaluated by microscopic observations.…”

Section: Cellular Biomarkers On Coelomocytesmentioning

confidence: 99%

“…Using caged organisms in biomonitoring studies, as well as in related research, makes it easier to standardize the results and to compare control organisms to animals collected from potentially polluted sites. The application of the biomarker approach to a selected earthworm population exposed to the test soil in laboratory standardized conditions offers a complementary approach to standard toxicity tests (i.e., mortality and reproduction rates) to investigate the effects of contaminant toxicity on living organisms at earlier stages and lower concentrations (Lukkari et al 2004;Gastaldi et al 2007;Schreck et al 2008). Appropriate biomarkers may be applied in standardized bioassays to provide evidence of the cause-effect relationship between soil contaminants and toxic effects in the individuals.…”

Section: Earthworm Biomarker Relevance For Soil Pollution Monitoring mentioning

confidence: 99%

Earthworm Biomarkers as Tools for Soil Pollution Assessment

Lionetto¹,

Calisi²,

Schettino³

2012

Soil Health and Land Use Management

View full text Add to dashboard Cite

Application of a biomarker battery for the evaluation of the sublethal effects of pollutants in the earthworm Eisenia andrei

Cited by 44 publications

References 39 publications

Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters

Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters

Enzymes of Earthworm as Indicators of Pesticide Pollution in Soil

Earthworm Biomarkers as Tools for Soil Pollution Assessment

Contact Info

Product

Resources

About