Application of an Efficient Amperometric Glucose Sensing Electrode Based on a Bilayer Polymer Film Platform

Abstract

A new approach was developed using a combination of a conducting polymer; poly(3,4-ethylenedioxythiophene) (PEDOT) with the electrochemically produced polymer of N-ferrocenyl-3-(1H-pyrrol-1-yl)aniline, (PFcPyBz) layer for the enzyme scaffolding resulting in excellent analytical parameters. To organize such a surface, graphite electrode was coated with a PEDOT layer and it was used as a transducer for electrochemical deposition of the polymer of a newly synthesized FcPyBz monomer. Using a PEDOT layer as the working electrode improved localization of the PFcPyBz on the transducer surface while enhancing the biosensor performance. A simple binding of glucose oxidase (GOx) as a test enzyme on this new polymeric platform was achieved using glutaraldehyde (GA) as the cross linker. The low limit of detection and high sensing sensitivity on glucose for the biosensor are estimated as 54 μM and 112.2 μA/mMcm 2 , respectively. The surface characterizations of the modified electrodes were investigated by cyclic voltammetry (CV), attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy and scanning electron microscopy (SEM) techniques. Finally, different kinds of beverages were tested for sensor reliability with high accuracy.

Thermoplastic Electrode (TPE)‐based Enzymatic Glucose Sensor Using Polycaprolactone‐graphite Composites

“…Effective immobilization of enzymes on electrode surfaces is crucial to developing effective biosensors. There are a wide variety of immobilization techniques including physical adsorption [7], entrapment in a semipermeable membrane [8], covalent bonding [9], crosslinking [10], and electrochemical polymerization [11]. Many of these techniques can be tedious, complicated, or the modification can be easily damaged [5d].…”

Conducting Polymers in Amperometric Sensors: A State of the Art over the Last 15 Years with a Focus on Polypyrrole-, Polythiophene-, and Poly(3,4-ethylenedioxythiophene)-Based Materials

“…On the other hand, N-ferrocenyl-3-(1H-pyrrol-1-yl)aniline has proved to allow good performances in terms of detection limit and sensitivity (54 µM and 112.2 µA/mM cm 2 , respectively) in detection of glucose. The sensor was finally tested in commercially available beverages (ice tea, lemonade, and milk samples), leading to an accurate detection of glucose concentration with relative error between 2.61 and 3.95% [114].…”

A Novel Amperometric Glucose Biosensor Based on Fe3O4‐Chitosan‐β‐Cyclodextrin/MWCNTs Nanobiocomposite

“…The effect of the concentration of GOx (from 1 to 9 mg mL À 1 ) on electrode response was examined and shown in Figure 6b. The weaker amperometric response at the GOx of concentrations lower than 5 mg mL À 1 might be due to the fact that the quantity of immobilized enzyme is too small to give full play to its catalytic effect, while the declining response at concentrations higher than 5 mg mL À 1 might be attributed be that too much non-conductive enzyme can hinder the transfer of electrons [41]. Figure 6c showed the influence of temperature on amperometric response.…”