Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine

Yuan, Kailong; Weng, Qianfeng; Zhang, Hongying; Xiong, Ji; Xu, Guowang

doi:10.1016/j.jpba.2004.12.009

Cited by 33 publications

(16 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several methods based on high performance liquid chromatography (HPLC) [10-12], capillary electrophoresis [13], liquid chromatography tandem mass spectrometry (LC-MS/MS) [14] and matrix assisted laser desorption/ionization (MALDI) [15] were reported for quantification of curcumin and its analogs from turmeric powder, food products, rat plasma, and other biological matrices. Recently, a simple and useful method for the analysis of curcumin analogs from rat plasma samples using solvent extraction and negative mode LC-MS/MS was reported [16], and this method can be efficiently used for the rapid screening of curcumin and THC [16].…”

Section: 0 Introductionmentioning

confidence: 99%

A liquid chromatography–tandem mass spectrometric method for quantification of curcuminoids in cell medium and mouse plasma

Saradhi

Ling

Wang

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

Curcumin and tetrahydrocurcumin (THC) have been found as potent DNMT1 inhibitors, but they suffer from low oral bioavailability and rapid metabolism in vivo. To circumvent these problems, two curcumin analogs: 1,7-bis(3,4-dimethoxyphenyl)-4,4-dimethyl-1,6-heptadiene-3,5-dione (TMC) and 1,7-bis(3,4-dimethoxyphenyl)-4-cyclohexyl-1,6-heptadiene-3,5-dione (DMCHC) have been synthesized to enhance their stability by blocking the two metabolic sites, the phenolic and C4 methylene moieties. Both compounds have shown inhibitory activity on M. SssI similar to that of curcumin and THC (Poster, M1114, AAPS, 2009. Preclinical pharmacokinetics has yet to be performed. In this paper, a simple liquid chromatography-tandem mass spectrometric method was developed for the determination of these four curcuminoids in cell medium and mouse plasma. The method showed linearity from 1 to 1000 ng/mL with the lower limit of quantification of 1 ng/ mL in cell medium, and 5 ng/mL in mouse plasma for all test curcuminoids. The within-day coefficients of variation were found to be below 15% and the accuracy was in the range of 85% to 115%. This method was subsequently used to evaluate their stability in these matrices and a pilot pharmacokinetics of curcumin, DMCHC and TMC in mice after an intraperitoneal (I.P.) cassette dosing of 10 mg/kg each. Curcuminoids degraded in two phases with terminal half lives of 186, 813, 724, and 2000 min for curcumin, THC, TMC, and DMCHC, respectively, in cell culture medium. In plasma, their respective half lives were 111, 232, 1202 and 3000 min. These data demonstrated that their stability is in the order curcumin

show abstract

Section: 0 Introductionmentioning

confidence: 99%

A liquid chromatography–tandem mass spectrometric method for quantification of curcuminoids in cell medium and mouse plasma

Saradhi

Ling

Wang

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

show abstract

“…However, it is not possible to quantify the individual curcuminoids with spectrophotometric methods. Estimation of curcuminoids has been reported by thin-layer chromatography method (TLC) [14], high performance thin-layer chromatography (HPTLC) [15,16], nearinfrared spectroscopic analysis [17], microemulsion electrokinetic chromatography [18], LC-ESI-MS/MS [19], capillary electrophoresis [20,21] and supercritical fluid chromatography [22]. But none of the reports above described statistical validation of chromatographic method for simultaneous determination of curcuminoids in C. longa.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of UPLC method for quality control of Curcuma longa Linn.: Fast simultaneous quantitation of three curcuminoids

Jin

Kong

Luo

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

“…The running buffer is composed of 15mM phosphate buffer at pH 9.7 [98]. Yuan et al (2005) developed a new capillary zone electrophoresis method coupled with diode array detection to simultaneously determine curcumin, demethoxycurcumin, and bisdemethoxycurcumin [99]. The curcuminoids were successfully separated within 7 min with a 15 mM sodium tetraborate buffer containing 10% methanol at pH 10.8.…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Chemical Analysis of the Chinese Herbal Medicine Turmeric (Curcuma longa L.)

Li¹,

Xiang²,

Xing³

et al. 2010

CPA

View full text Add to dashboard Cite

Turmeric (Curcuma longa L., Zingiberaceae family) is a well known Chinese herbal medicine. It possesses diverse pharmacologic effects including antioxidant, anti-inflammatory, antiproliferative and antiangiogenic activities. The main bioactive constituents of C. longa include sesquiterpenes, which are responsible for its aroma, and curcuminoids, which contribute to its bright yellow color. Both sesquiterpenes and curcuminoids exhibit great potential as therapeutic agents for various diseases including colon cancer, pancreatic cancer, diabetes and Alzheimer's disease. Particularly, curcumin, one of the major curcuminoids of turmeric, has been proved to be effective in cancer treatment and prevention by recent phase I clinical trials. In order to ensure the efficacy and safety of turmeric in clinical applications, sensitive and accurate analytical methods are of significance for the comprehensive quality control of C. longa. This review summarizes recent progress in the chemical analysis of sesquiterpenes and curcuminoids in turmeric.

show abstract

Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine

Cited by 33 publications

References 13 publications

A liquid chromatography–tandem mass spectrometric method for quantification of curcuminoids in cell medium and mouse plasma

A liquid chromatography–tandem mass spectrometric method for quantification of curcuminoids in cell medium and mouse plasma

Development and validation of UPLC method for quality control of Curcuma longa Linn.: Fast simultaneous quantitation of three curcuminoids

Chemical Analysis of the Chinese Herbal Medicine Turmeric (Curcuma longa L.)

Contact Info

Product

Resources

About