Application of Chitosan/PVA Nano fiber as a potential wound dressing for streptozotocin-induced diabetic rats

Majd, Saeed Ahmadi; Khorasgani, Mohammad Rabbani; Moshtaghian, Seyed Jamal; Talebi, Ardeshir; Khezri, Mohammad

doi:10.1016/j.ijbiomac.2016.06.035

Cited by 100 publications

(49 citation statements)

References 13 publications

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“…Polyvinyl alcohol is a distinctive category of fiber because it is easily coagulated, spun, cross-linked, and oriented [ 22 ]. Previously, great efforts have been put in place to facilitate fabrication of a high surface area nanofiber blended with CHT/PVA using the electrospinning technique [ 23 , 24 , 25 ]. However, both expensive and toxic chemicals like dichloromethane, trifluoroacetic acid (TFA), acetic acid, and chloroform may be used, but their residues negatively impact the health of the user and the environment in general, thus they cannot be categorized as green nanofibers.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Synthesis and Characterization of a Novel “Green” H2O2-Assisted, Water-Soluble Chitosan/Polyvinyl Alcohol Nanofiber for Environmental End Uses

Pervez

Stylios

2018

Nanomaterials

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The present work highlights the formation of a novel green nanofiber based on H2O2-assisted water-soluble chitosan/polyvinyl alcohol (WSCHT/PVA) by using water as an ecofriendly solvent and genipin used as a nontoxic cross-linker. The 20/80 blend ratio was found to have the most optimum uniform fiber morphology. WSCHT retained the same structure as WISCHT. The prepared nanofibers were characterized by Scanning electron microscopy (SEM), Fourier transform spectroscopy (FTIR), Thermo gravimetric analysis (TGA), Differential scanning calorimeter (DSC), X-ray diffraction (XRD), Water Contact Angle (WCA) and Ultraviolet-visible spectroscopy (UV-vis). During electrospinning, the crystalline microstructure of the WSCHT/PVA underwent better solidification and after cross-linking there was an increase in the melting temperature of the fiber. Swelling ratio studies revealed noticeable increase in hydrophilicity with increase of WSCHT, which was further demonstrated by the decrease of contact angle from 64.74° to 14.68°. WSCHT/PVA nanofiber mats exhibit excellent UV blocking protection with less than 5% transmittance value and also showed improved in vitro drug release properties with stable release for longer duration (cross-linked fibers) and burst release for shorter duration (uncross linked) fibers. Finally our experimental data demonstrates excellent adsorption ability of Colour Index (C.I.) reactive black 5 (RB5) due to protonated amino groups.

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Section: Introductionmentioning

confidence: 99%

Investigating the Synthesis and Characterization of a Novel “Green” H2O2-Assisted, Water-Soluble Chitosan/Polyvinyl Alcohol Nanofiber for Environmental End Uses

Pervez

Stylios

2018

Nanomaterials

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“…Based on the results of the present study, the mean diameter of CS/PVA (25:75) nanofibers was 238.36 ± 36.99 nm. According to previous studies, by using different CS/PVA ratios for the fabrication of nanofibers (20:80, 27 25:75, 10 and 75:25 28 ), the diameter of CS/PVA nanofibers equals 100, 200.22, and 279.84 nm, respectively. A positive relationship appears to exist between the nanofiber diameter and the CS content 10 .…”

Section: Resultsmentioning

confidence: 96%

Effects of bilayer nanofibrillar scaffolds containing epidermal growth factor on full‐thickness wound healing

Golchin

Nourani

2020

Polymers for Advanced Techs

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Wound healing refers to a complex, dynamic process in the body, especially the skin, which influences the quality of life of millions of people each year. Electrospinning is an efficient and economic technique for the production of micro-to nano-fibrillar constructs as drug delivery systems with biomedical applications. In this study, we designed an electrospun bilayer fibrillar scaffold immobilized with epidermal growth factor (EGF), using polycaprolactone (PCL) as the upper layer and chitosan (CS)/polyvinyl alcohol (PVA) as the lower layer of wound dressing. The scanning electron microscopy (SEM) was utilized to characterize the scaffolds. The water uptake, release potency, and toxicity of scaffolds were evaluated, and also then DAPI staining and MTT assay after seeding adipose tissue-derived mesenchymal stem cells. Finally, the effects of the scaffolds on the wound healing process were investigated in a mouse model of full-thickness skin injury. The results of SEM demonstrated continuous, smooth, bead-free, and randomly aligned fibers. The average diameter of CS/PVA nanofibers was 238.36 ± 36.99, and the mean diameter of PCL nanofibers was 1271.79 ± 428.49 nm. The scaffolds were capable of cell seeding and supported their growth and proliferation. Furthermore, in the comparison between designed composite as dressing patch and control group (Vaseline), our composite demonstrated significant differences, so that EGF-immobilized patch efficiently accelerated wound closure and improved histological healing in an in vivo full-thickness wound healing mouse model. The present results suggest the potential of the designed electrospun bilayer fibrillar scaffold containing EGF for the treatment of fullthickness wounds and skin regeneration. K E Y W O R D S chitosan/polyvinyl alcohol/polycaprolactone, epidermal growth factor (EGF), mesenchymal stem cells, skin regeneration, wound healing 1 | INTRODUCTION Skin is one of the vital body tissues, which is composed of dermis, epidermis, and hypoderm (subcutaneous fat) layers and covers the external body surface. It constitutes almost 8% of the total mass of the body and has several crucial functions. 1 It provides an effective barrier against adverse external conditions and has properties, which can prevent microbial, mechanical, chemical, osmotic, thermal, and photo damage. Skin wounds, which can be caused by burns, venous ulcers, diabetic ulcers, and acute injury, are categorized into several types. Generally, wound healing is a dynamic and complex process, which is able to restore the skin's routine functionality and anatomical

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“…Fibers obtained from electrospinning have gained popularity in the field of drug delivery and are considered as ideal dressing materials for non-healing wounds since the method is versatile and can deliver various biological agents long-term to local tissues at the wound site [ 26 , 27 , 28 ]. Not only do they provide physical protection to the wound, but they also have the capacity to be incorporated with a high amount of drugs (up to 40% loading), where the release of which can be adjusted by changing the types and compositions of the materials in the fibers [ 29 ].…”

Section: Electrospun Fibersmentioning

confidence: 99%

“…PVA fibers rapidly disintegrate in aqueous solutions resulting in the fast release of drug due to dissolution of the carrier materials [ 70 ]. PVA is quite compatible with chitosan as suggested by a study using blend fibers of PVA/chitosan for dressing materials on diabetic wounds [ 28 ]. Results showed that the rats treated with PVA/chitosan fiber dressings had an increased wound healing rate as compared to those untreated rats.…”

Section: Electrospun Fibersmentioning

confidence: 99%

Electrospun Fibers as a Dressing Material for Drug and Biological Agent Delivery in Wound Healing Applications

et al. 2018

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Wound healing is a complex tissue regeneration process that promotes the growth of new tissue to provide the body with the necessary barrier from the outside environment. In the class of non-healing wounds, diabetic wounds, and ulcers, dressing materials that are available clinically (e.g., gels and creams) have demonstrated only a slow improvement with current available technologies. Among all available current technologies, electrospun fibers exhibit several characteristics that may provide novel replacement dressing materials for the above-mentioned wounds. Therefore, in this review, we focus on recent achievements in electrospun drug-eluting fibers for wound healing applications. In particular, we review drug release, including small molecule drugs, proteins and peptides, and gene vectors from electrospun fibers with respect to wound healing. Furthermore, we provide an overview on multifunctional dressing materials based on electrospun fibers, including those that are capable of achieving wound debridement and wound healing simultaneously as well as multi-drugs loading/types suitable for various stages of the healing process. Our review provides important and sufficient information to inform the field in development of fiber-based dressing materials for clinical treatment of non-healing wounds.

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Application of Chitosan/PVA Nano fiber as a potential wound dressing for streptozotocin-induced diabetic rats

Cited by 100 publications

References 13 publications

Investigating the Synthesis and Characterization of a Novel “Green” H2O2-Assisted, Water-Soluble Chitosan/Polyvinyl Alcohol Nanofiber for Environmental End Uses

Investigating the Synthesis and Characterization of a Novel “Green” H2O2-Assisted, Water-Soluble Chitosan/Polyvinyl Alcohol Nanofiber for Environmental End Uses

Effects of bilayer nanofibrillar scaffolds containing epidermal growth factor on full‐thickness wound healing

Electrospun Fibers as a Dressing Material for Drug and Biological Agent Delivery in Wound Healing Applications

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