“…such, the effects of varying ratios of CTAB to TEOS, with or without ethanol, were investigated in our study. The former could affect the mesoporous pore and particle sizes of the MSNs, while the latter could improve the dispersity of MSNs [31]. The cellular uptake results were consistent with the expected objective that modification of FA significantly improves the endocytosis of nanoparticles by its targeting role.…”
The objective of this study was to construct norcantharidin (NCTD)/tetrandrine (Tet) dual-drug loaded lipid nanoparticles (FA-LP@Tet/(MSNs@NCTD)) based on mesoporous silica nanoparticles (MSNs) for controlling drug release and lowering their systemic toxicity. In this study, MSNs were prepared and used for encapsulating anticancer drug NCTD; then MSNs@NCTD and Tet were loaded into liposomes to construct dual-drug loaded lipid nanoparticles with folic acid (FA) as the targeting moiety. The prepared dual-drug loaded lipid nanoparticles with a uniform particle size distribution of 153.17±4.17 nm (PDI 0.191±0.017, zeta potential -20.93±1.75 mV), had a visible core-shell structure under transmission electron microscopy; the encapsulation efficiency of NCTD and Tet was 86.62% and 79.19%, respectively, with obvious in vitro sustained release characteristics. The cellular uptake results suggested that FA modification could enhance intracellular distribution of FA-LP@Tet/(MSNs@NCTD). Furthermore, cell apoptosis assays showed FA-LP@Tet/(MSNs@NCTD) had better antitumor ability via reversing multidrug resistance. Therefore, FA-LP@Tet/(MSNs@NCTD) was a promising drug delivery system for combination cancer therapy.
“…such, the effects of varying ratios of CTAB to TEOS, with or without ethanol, were investigated in our study. The former could affect the mesoporous pore and particle sizes of the MSNs, while the latter could improve the dispersity of MSNs [31]. The cellular uptake results were consistent with the expected objective that modification of FA significantly improves the endocytosis of nanoparticles by its targeting role.…”
The objective of this study was to construct norcantharidin (NCTD)/tetrandrine (Tet) dual-drug loaded lipid nanoparticles (FA-LP@Tet/(MSNs@NCTD)) based on mesoporous silica nanoparticles (MSNs) for controlling drug release and lowering their systemic toxicity. In this study, MSNs were prepared and used for encapsulating anticancer drug NCTD; then MSNs@NCTD and Tet were loaded into liposomes to construct dual-drug loaded lipid nanoparticles with folic acid (FA) as the targeting moiety. The prepared dual-drug loaded lipid nanoparticles with a uniform particle size distribution of 153.17±4.17 nm (PDI 0.191±0.017, zeta potential -20.93±1.75 mV), had a visible core-shell structure under transmission electron microscopy; the encapsulation efficiency of NCTD and Tet was 86.62% and 79.19%, respectively, with obvious in vitro sustained release characteristics. The cellular uptake results suggested that FA modification could enhance intracellular distribution of FA-LP@Tet/(MSNs@NCTD). Furthermore, cell apoptosis assays showed FA-LP@Tet/(MSNs@NCTD) had better antitumor ability via reversing multidrug resistance. Therefore, FA-LP@Tet/(MSNs@NCTD) was a promising drug delivery system for combination cancer therapy.
“…The release behaviour of DOX was investigated at pH 7.4 (physiological condition in the blood) and 4.5 (environmental condition around cancer cells) by UV-visible spectroscopy method [15]. Briefly, 1 mg/mL of DOX/FTN was dispersed in PBS with different pH and kept in the dark at 37 C. At predetermined time intervals, supernatants were collected and the absorptions were measured at 480 nm.…”
Theranostic nanoparticles have attracted considerable attention in recently revolutionized medicine. Since the last decade, there has been a growing attempt to design various theranostic nanoparticles but difficulties still exist in the fabrication of their biocompatible one. Herein, fluorescent titania nanoparticles (FTN) were fabricated using a one-step green method. This FTN had ultra-high doxorubicin hydrochloride (DOX) loading capacity (encapsulation efficiency 95.50% and loading content 38.20%) that release the loaded drug in response to acidic pH. In vitro cytotoxicity experiments on human osteosarcoma (SaOs-2) and breast cancer (MCF-7) cell lines revealed superior anticancer efficacy (lowered the IC concentration by 3- and 5.5-fold for SaOs-2 and MCF-7 cells, respectively) and also better imaging for intracellular tracking of DOX/FTN relative to free DOX. Furthermore, the prepared nanoparticles showed efficient antibacterial activity against both gram-negative (Escherichia coli ATCC 25922) and gram-positive (Staphylococcus aureus ATCC 25923) bacteria. In this study, we have developed novel theranostic titania nanoparticles with inherent fluorescence property for cancer imaging and therapy.
“…The peak at around 950 cm −1 can be assigned to Si–OH bending mode. The sharp peak at 1087–1095 cm −1 corresponds to Si–O–Si vibrations 45 . The above observation clearly shows that application of sol-gel coating does allow to fabricate well-defined spherical capsules with enhanced mechanical strength.Figure 1The schematic illustration of the intracellular delivery of antiviral siRNA against influenza A virus using SiO 2 -coated hybrid capsules.Figure 2( A ) The schematic illustration of the fabrication of SiO 2 -coated capsules and the encapsulation of antiviral siRNA.…”
The implementation of RNAi technology into the clinical practice has been significantly postponing due to the issues regarding to the delivery of naked siRNA predominantly to target cells. Here we report the approach to enhance the efficiency of siRNA delivery by encapsulating the siRNA into new carrier systems which are obtained via the combination of widely used layer-by-layer technique and in situ modification by sol-gel chemistry. We used three types of siRNAs (NP-717, NP-1155 and NP-1496) in encapsulated form as new therapeutic agents against H1N1 influenza virus infection. By employing the hybrid microcontainers for the siRNA encapsulation we demonstrate the reduction of viral nucleoprotein (NP) level and inhibition of influenza virus production in infected cell lines (MDCK and A549). The obtained hybrid carriers based on assembled biodegradable polyelectrolytes and sol-gel coating possess several advantages such as a high cell uptake efficiency, low toxicity, efficient intracellular delivery of siRNAs and the protection of siRNAs from premature degradation before reaching the target cells. These findings underpin a great potential of versatile microencapsulation technology for the development of anti-viral RNAi delivery systems against influenza virus infection.
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