Application of High-Speed Countercurrent Chromatography/Electrospray Ionization Mass Spectrometry (HSCCC/ESIMS) in Natural Products Chemistry

Abstract

In this paper, we describe the technique of analytical highspeed countercurrent chromatography coupled with electrospray ionization mass spectrometry (HSCCCESIMS) for the first time, and evaluate its performance in terms of chromatography and mass spectrometry. This technique demonstrates the capability and resolving power of HSCCC in the separation of a few natural product mixtures and utilizes ESIMS as a detector with continuous detection and high sensitivity. Application of this HSCCC/ESIMS system is illustrated by the separation and detection of biologically important compounds. 65

Application of atmospheric pressure chemical ionisation mass spectrometry in the analysis of barbiturates by high‐speed analytical countercurrent chromatography

“…The advent of mass spectrometry as a viable CCC detection system has allowed HSACCC to become a powerful analytical method in many areas of application. There have only been a few CCC/MS systems reported in the literature up to this time; for example, the thermospray system of Lee and Voyksner,11 the frit electron ionisation (EI) system of Oka and co‐workers,12 the electrospray systems of Kong et al 13. and our own efforts 14.…”

Hydrogen‐Bond‐Mediated Asymmetric Cascade Reaction of Stable Sulfur Ylides with Nitroolefins: Scope, Application and Mechanism

“…Several papers have highlighted the potential of CCC techniques coupled to MS and pointed out that the combined technique integrates the advantages of the two separate techniques, i.e., the separation power of CCC and the highly specific and low detection limits of MS 16–23. Passive flow‐splitters such as T‐ or Y‐split connections have most often been employed to combine CCC to fast‐atom bombardment, chemical ionization and ESI sources associated to the MS analyzer 16, 18, 22.…”

“…However, several points that have been underlined are still problematic in CCC/MS. Thus, high back‐pressure caused by lower strength connection tubing often occurs, generating dramatic effects such as leakage or rupturing of the chromatographic column and disturbance of the MS source 19, 20. Even though some ionization techniques such as thermospray have been shown to reduce this constraint,16 the fact remains that these difficulties usually limit investigations.…”