Application of Immunohistochemistry to the Diagnosis of Salivary Gland Tumors

Araújo, Vera Cavalcanti de; Sousa, Suzana Orsini Machado de; Carvalho, Yasmin Rodarte; Araújo, Ney Soares de

doi:10.1097/00129039-200009000-00005

Cited by 74 publications

(88 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When the lesion predominantly consists of clear cells, it might be confused with salivary gland tumors such as oncocytoma and mucoepidermoid carcinoma, in addition to metastatic renal carcinomas, clear cell odontogenic carcinomas, and peripheral ameloblastomas. 3,20 In agreement with Bouckaert et al, 4 the morphological findings of peripheral CEOT led us to infer that this lesion arises from the oral epithelium because the cells of these tumors often resemble squamous cells. In the present case, the morphological characteristics observed left no doubt that the lesion was a peripheral CEOT because we observed proliferation of hyperpigmented epithelial cells arranged in islets and chains, foci of basophilic material resembling dystrophic calcifications, some of them showing concentric arrangements characteristic of Liesegang rings, in addition to the presence of amorphous eosinophilic material and the absence of radiographic signs.…”

Section: Discussionsupporting

confidence: 85%

Peripheral Calcifying Epithelial Odontogenic Tumor Associated With Generalized Drug-Induced Gingival Growth: A Case Report

Silveira

Gordón-Núñez

Seabra

et al. 2007

Journal of Oral and Maxillofacial Surgery

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 85%

Peripheral Calcifying Epithelial Odontogenic Tumor Associated With Generalized Drug-Induced Gingival Growth: A Case Report

Silveira

Gordón-Núñez

Seabra

et al. 2007

Journal of Oral and Maxillofacial Surgery

View full text Add to dashboard Cite

“…This complexity has been attributed to the myoepithelial component of these tumors (1). In view of the difficulty in identifying these myoepithelial cells by routine hematoxylin-eosin staining and even by special techniques, immunohistochemistry has been found to be a useful tool in the distinction of these cells, contributing to an improved differential diagnosis of salivary gland tumors (2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

“…Vimentin has been widely employed as a specific marker for neoplastic myoepithelial cells (12), although this specificity has subsequently been reconsidered (2). At the same time, the literature reported the use of glial fibrillary acid protein and protein S-100 for the identification of these cells, especially in normal salivary glands (13).…”

Section: Introductionmentioning

confidence: 99%

“…At the same time, the literature reported the use of glial fibrillary acid protein and protein S-100 for the identification of these cells, especially in normal salivary glands (13). Various other proteins have currently been employed for the same purpose in salivary gland tumors, including calponin, h-caldesmon, smooth muscle α-actin, muscle-specific actin, smooth muscle myosin heavy chain and c-kit (2,3,8,9,14), although with controversial results.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors

et al. 2007

View full text Add to dashboard Cite

Myoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors.

show abstract

“…Although the expression patterns of CK7, CK8, CK14, CK19, a-smooth muscle actin (aSMA), caldesmon and calponin have been reported already, [2][3][4][5][6][7] there are only a few reports, or none, describing the expression of p63, 4,8 maspin, 14-3-3s, CD10 9 and CD44v6 10 in salivary gland tumors. Briefly, the putative functions of these molecules are summarized as follows.…”

mentioning

confidence: 99%

Phenotypic composition of salivary gland tumors: an application of principle component analysis to tissue microarray data

et al. 2004

View full text Add to dashboard Cite

The tissue organization of the salivary gland is complex, and a large number of salivary gland tumor entities with a broad morphologic spectrum are listed, creating tumor classification schema for the salivary glands that are difficult to understand. In the present study, we attempted to examine how the anatomical components of the salivary gland are associated with morphological subtypes of tumors. We selected a panel of 12 molecules, which labeled one or some of the components, with all of the markers covering every component of the salivary glands. Using tissue microarray, expression profiles of these molecules were examined in four representative spots from each of 88 salivary gland tumors. The resulting large data matrix was analyzed using principle component analysis (PCA). We considered the first three eigenvectors to be significant; as the eigenvalues were more than 1.0 and the cumulative proportion achieved was 67%. Comparison with expression patterns in normal tissue suggested that the three components represented myoepithelial differentiation, and luminal and basal cell phenotypes. Then, we compared the PCA results with individual morphologic subtypes. Individual subtypes were clustered among the three dimensions of the components. This implies that salivary gland tumors may be well characterized by using only three components.

show abstract

Application of Immunohistochemistry to the Diagnosis of Salivary Gland Tumors

Cited by 74 publications

References 72 publications

Peripheral Calcifying Epithelial Odontogenic Tumor Associated With Generalized Drug-Induced Gingival Growth: A Case Report

Peripheral Calcifying Epithelial Odontogenic Tumor Associated With Generalized Drug-Induced Gingival Growth: A Case Report

Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors

Phenotypic composition of salivary gland tumors: an application of principle component analysis to tissue microarray data

Contact Info

Product

Resources

About