Application of in-vivo laser scanning microscopy for evaluation
of barrier creams

Rieger, Tina; Teichmann, A.; Richter, Heike; Sterry, Wolfram; Lademann, Juergen

doi:10.1002/lapl.200610076

Cited by 24 publications

(22 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis of the penetration of topically applied substances into the skin is important for the development and optimization of topically applied drugs and cosmetic products [1]. In the past, it was assumed that the intercellular penetration -i.e., the diffusion of topically applied substances in the lipid layers surrounding the corneocytesis the most important penetration pathway [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the penetration profiles of topically applied substances by laser scanning microscopy

Martschick

Teichmann²,

Richter³

et al. 2006

Laser Phys. Lett.

Self Cite

View full text Add to dashboard Cite

Tape stripping in combination with spectroscopic measurements is a suitable method for the non-invasive determination of the dermatopharmacokinetics of topically applied substances. The penetration profiles obtained by these methods represent a cut to the stratum corneum, where the distribution of the topically applied substances is shown. For the penetration of these penetration profiles knowledge regarding the penetration pathways is necessary. Laser scanning microscopy is a well-suited method for the analysis of the penetration pathways. In the present paper, the results obtained by the combined tape stripping method and laser scanning microscopy were compared for the interpretation of penetration profiles concerning the penetration pathways of the topically applied substances. µmDistribution of the dye in hair follicles

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of the penetration profiles of topically applied substances by laser scanning microscopy

Martschick

Teichmann²,

Richter³

et al. 2006

Laser Phys. Lett.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The basic station is connected to a hand-held device by optical fibers. The optical system and the focus tuning unit are integrated into the hand-held device [16,17]. This hand-held device can be used to investigate all body sites.…”

Section: Laser Scanning Microscopymentioning

confidence: 99%

In vivovisualization of microneedle conduits in human skin using laser scanning microscopy

Bal¹,

Kruithof²,

Liebl³

et al. 2010

Laser Phys. Lett.

View full text Add to dashboard Cite

Solid microneedles enhance the penetration of drugs into the viable skin but little is known about the geometry of the conduits in vivo. Therefore, laser scanning microscopy was used to visualize the conduits of a microneedle system with needles at a length of 300 μm in 6 healthy subjects over a period of time. The model drug, a fluorescent dye was applied before and after piercing. Laser scanning microscopy was evaluated as being an excellent method to monitor the geometry and closure of the conduits over time. The used microneedle system was evaluated as suitable to enhance the transport of model drugs into the viable epidermis without bleeding and a short closure time of the conduits at the skin surface.

show abstract

“…The analysis of the skin properties with optical and spectroscopic methods is of interest, both in the field of cosmetology as well as in the area of diagnostics and therapy control of skin diseases. Especially in the field of cosmetology, optical and spectroscopic methods have already been successfully availed to characterize diverse skin parameters, such as the dryness and oiliness of the skin [1,2], the barrier function of the skin [3,4,5] the depth and density of furrows and wrinkles [6], and the homogeneity of distribution of topically applied creams, as well as their penetration into the skin [7,8].…”

Section: Introductionmentioning

confidence: 99%

Application of optical non-invasive methods in skin physiology

et al. 2008

Self Cite

View full text Add to dashboard Cite

Abstract:In the present paper the application of optical noninvasive methods in dermatology and cosmetology is discussed. Laser scanning microscopy (LSM) and optical coherent tomography (OCT) are the most promising methods for this application. Using these methods, the analysis of different skin parameters like dryness and oiliness of the skin, the barrier function and the structure of furrows and wrinkles are discussed. Additionally the homogeneity of distribution of topically applied creams, as well as their penetration into the skin were investigated. It is shown that these methods are highly valuable in dermatology for diagnostic and therapy control and for basic research, for instance in the field of structure analysis of hair follicles and sweat glands. The vertical images of the tissue produced by OCT can be easily compared with histological sections. Unfortunately, the resolution of the OCT technique is not high enough to carry out measurements on a cellular level, as is possible by LSM. LSM has the advantage that it can be used for the investigation of penetration and storage processes of topically applied substances, if these substances have fluorescent properties or if they are fluorescentlabelled.In vivo application of a fiber-based OCT-system for diagnostic and therapy control in the case of hair diseases

show abstract

Application of in-vivo laser scanning microscopy for evaluation of barrier creams

Cited by 24 publications

References 20 publications

Analysis of the penetration profiles of topically applied substances by laser scanning microscopy

Analysis of the penetration profiles of topically applied substances by laser scanning microscopy

In vivovisualization of microneedle conduits in human skin using laser scanning microscopy

Application of optical non-invasive methods in skin physiology

Contact Info

Product

Resources

About