Application of Ionic Liquid as a Green Solvent for Polyphenolics Content Extraction of Peperomia pellucida (L) Kunth Herb

Ahmad, Islamudin; Yanuar, Arry; Mulia, Kamarza; Mun’im, Abdul

doi:10.5530/jyp.2017.9.95

Cited by 22 publications

(26 citation statements)

References 15 publications

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“…In addition, these profiles correlate with total polyphenolics content from extracts of each method. 13,23 Therefore, only the optimum conditions obtained from the instrument used to separate the class of compounds contained in the extract based on the technique used, and the preliminary data related to the use of liquid ionic solvents as an alternative solution with a green chemical principles approach.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling Analysis of Conventional and Non-Conventional Extraction Methods on Secondary Metabolite from Peperomia pellucida (L.) Kunth Using UPLC-QToF-MS/MS System

Ahmad¹,

Mulia²,

Yanuar³

et al. 2018

JYP

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Section: Discussionmentioning

confidence: 99%

Metabolite Profiling Analysis of Conventional and Non-Conventional Extraction Methods on Secondary Metabolite from Peperomia pellucida (L.) Kunth Using UPLC-QToF-MS/MS System

Ahmad¹,

Mulia²,

Yanuar³

et al. 2018

JYP

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“…While previous studies, on trans-resveratrol content has been extracted from optimum IL-MAE condition 2.5 M BmimBr solution, 15 mL/g liquid/solid ratio, 10% microwave power, and 10 min extraction time (Ayuningtyas et al, 2017), on the yield of total polyphenolics content, IL-MAE 0.7 M BmimCl (14 ml/l liquid-solid ratio, and 270 Watts microwave power for 10 minutes) better than 0.7 M BmimBr (14 ml/l liquid-solid ratio, and 270 Watts microwave power for 15 minutes) (Ahmad et al, 2017).…”

Section: High-performance Liquid Chromatography (Hplc) Analysis Of Oxmentioning

confidence: 94%

“…The efficiency of the IL-MAE method was much higher than the maceration method that caused damage to the cell wall surface of the sample matrix by microwave irradiation interference (Ahmad et al, 2017). IL-MAE method and salting out an improved yield of oxyresveratrol from M. alba roots significantly.…”

Section: High-performance Liquid Chromatography (Hplc) Analysis Of Oxmentioning

confidence: 96%

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Ionic Liquid-based Microwave-Assisted Extraction (Il-MAE) of Oxyresveratrol from Morus alba Roots

Fadhila

Mun’im²,

Jufri³

2018

J App Pharm Sci

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The objective of this study was to investigate the efficacy of using ionic liquid-based microwave-assisted (IL-MAE) approach for extraction of oxyresveratrol from Morus alba roots and to compare the result with conventional extraction. The roots were extracted with nine IL, 1-butyl-3-methylimidazolium chloride (BmimCl), 1-butyl-3-methylimidazolium bromide (BmimBr), or 1-butyl-3-methylimidazolium tetrafluoroborate (BmimBF 4 ) at concentration 1.5, 1.67, and 2 M for each IL, in a microwave at Power 30% for 10 minutes respectively. The extracts were filtrated and diluted with 30 ml of deionized water. Then, fractionated with ethyl acetate and salting out by addition of Na 2 CO 3 or KH 2 PO 4 . The fractions were analyzed for oxyresveratrol contents with TLC-densitometer and HPLC. Then the result was compared with conventional methods. IL-MAE approach showed improvement extraction efficiency compared to the conventional ethanol and methanol macerations. The optimized solvent condition for extraction of oxyresveratrol from Morus alba root was 1.5 M BmimCl with 0.01 M KH 2 PO 4 salt. Oxyresveratrol was separated by ethyl acetate fractionation, and the yield calculated was 3.53% from raw material. The study showed that IL-MAE was an efficient method for the extraction of oxyresveratrol from Morus alba roots.

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“…Total phenolic content was determined using the 96-well microplate with Folin-Ciocalteu reagent, adapted from Ahmad et al 15 with slight modification. 20 μL of the diluted extract or standard solution were added to 100 μL of reagent 25% Foline-Ciocalteu and homogenized by shaken for 60 s in 96-well microplate, and then allowed to stand for 4 min.…”

Section: Determination Of Total Phenolic Contentmentioning

confidence: 99%

Inhibitory Effect on Arginase and Total Phenolic Content Determination of Extracts from Different parts of Melastoma malabathricum L.

Sembiring

Elya²,

Sauriasari³

2018

JYP

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The part of the plant were collected and cleaned, dried at room temperature, and then powdered and stored in an air tight glass container. 50 g of each plant part was extracted by maceration with 500 mL of 70% ethanol ABSTRACTBackground: Some phenolic compounds are potential source for arginase inhibitor that can be used in management of disease associated with endothelial dysfunction. Objective: The aim of this study was to investigate the inhibitory effect on arginase and to determine total phenolic content of extracts from different parts of Melastoma malabathricum L. Methods: Each part were extracted with 70% ethanol by maceration. The extracts were mixed with bovine liver arginase and L-arginine as a substrate. The inhibitory effect of extracts were determined in vitro, by measuring its absorbance on 430 nm. Total phenolic content were determined with Folin-Ciocalteu 25% on 750 nm. Results: The 70% ethanol extract of M. malabathricum leaves at 100 µg/mL was found to have highest inhibition (81.26 ± 5.27 %) followed by flower 73.39 ± 9.39%, fruit 67.63 ± 7.61 and stem 61.61 ± 4.56%. The IC 50 value of the most active extract was 62.43 µg/mL while the IC 50 value of N-hidroksi-nor-L-arginin (nor-NOHA) acetate, an arginase inhibitor was found to be 3.91 µg/mL. Total phenolic content of 70% ethanolic extracts of M. malabathricum leaves, flower, fruit and stem was 15.9, 20.7, 6.44, and 6.29%. Conclusion: The 70% ethanol extract of M. malabathricum leaves exhibited the highest inhibition on arginase but highest total phenolic content was from flower part. Pearson correlation test showed no correlation p>0.05 between arginase inhibition and total phenolic content of the samples.

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Application of Ionic Liquid as a Green Solvent for Polyphenolics Content Extraction of Peperomia pellucida (L) Kunth Herb

Cited by 22 publications

References 15 publications

Metabolite Profiling Analysis of Conventional and Non-Conventional Extraction Methods on Secondary Metabolite from Peperomia pellucida (L.) Kunth Using UPLC-QToF-MS/MS System

Metabolite Profiling Analysis of Conventional and Non-Conventional Extraction Methods on Secondary Metabolite from Peperomia pellucida (L.) Kunth Using UPLC-QToF-MS/MS System

Ionic Liquid-based Microwave-Assisted Extraction (Il-MAE) of Oxyresveratrol from Morus alba Roots

Inhibitory Effect on Arginase and Total Phenolic Content Determination of Extracts from Different parts of Melastoma malabathricum L.

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